Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Identification of SNP(Single Nucleotide Polymorphism) from MC1R, MITF and TYRP1 associated with Feather Color in Chicken

닭의 모색 연관 유전자인 MC1R, MITF, TYRP1의 SNP(Single Nucleotide Polymorphism) 규명

  • 김병기 (경상북도 축산기술연구소) ;
  • 변윤화 (영남대학교 생명공학부 생명공학과) ;
  • 하재정 (경상북도 축산기술연구소) ;
  • 정대진 (경상북도 축산기술연구소) ;
  • 이윤석 (서울대학교 그린바이오 과학기술연구원) ;
  • 형기은 (영남대학교 생명공학부 생명공학과) ;
  • 여정수 (영남대학교 생명공학부 생명공학과) ;
  • 오동엽 (경상북도 축산기술연구소)
  • Received : 2013.10.13
  • Accepted : 2014.03.04
  • Published : 2014.03.31
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Abstract

The Feather Color of chicken is considered as most obvious, and the purpose of this study is to identify the genotype following the SNP of MC1R, MITF and TYRP1, which are genes related to Feather Color, and develop a SNP marker that can be classified per breed. When a haplotype is observed through the combination of markers, a Korean Native Chicken can especially be distinguished when it is a CGG type in the SNP combination of the MC1R gene. In case of the TAG, TGG and TAA types, only Araucana was identified, and for the CAA type, Leghorn could specifically be distinguished. In the SNP combination of TYRP1 gene, only Leghorn was differentiated in case of the TTTCA and CCTCA types, and only Silky Fowl was identified in case of the CTTTA type. The SNP combination of MC1R gene enabled for Korean Native Chicken, Leghorn, and Araucana to be distinguished and each of the SNP and combination of TYRP1 gene allowed for all 4 breeds to be classified. If many researches are conducted about genetic polymorphism between breeds, then it is considered that the differences between breeds will be understood from a molecular biological aspect instead of simply distinguishing the breeds through Feather Color.

닭을 구분하는데 있어 가장 눈에 띠는 것이 모색이며, 모색 관련 유전자인 MC1R, MITF, TYRP1의 SNP에 따른 유전자형을 확인하고, 각 품종별로 구별이 가능한 SNP 마커를 개발하고자 하는데, 본 연구의 목적이 있다. 마커들을 조합으로 haplotype을 보았을 때, MC1R 유전자의 SNP 조합에서 CGG type일 경우, 재래닭 만을 특별히 구별할 수 있었으며, TAG, TGG, TAA type일 경우에는 아라카나 만을 구별할 수 있었고, CAA type의 경우, 레그혼 만을 특이적으로 구별할 수 있었다. TYRP1 유전자의 SNP 조합에서는 TTTCA, CCTCA type의 경우, 레그혼 만을 구별할 수 있으며, CTTTA type의 경우, 오골계 만을 특이적으로 구별할 수 있었다. MC1R 유전자의 SNP 조합으로 재래닭, 레그혼, 아라카나를 구별할 수 있었고, TYRP1 유전자의 각각의 SNP 및 조합으로 4가지 품종 모두 구별할 수 있었다. 이렇게 품종 간의 유전적 다형성에 대한 연구가 더 많이 진행된다면, 단순 모색만으로 품종을 구별하기보다는 분자생물학적으로 품종 간의 차이를 이해할 수 있게 될 것이라고 생각된다.

Keywords

References

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