Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Partial Purification and Properties of a Cysteine Protease from Citrus Red Mite Panonychus citri

  • Hong, Seong Chul (Department of Preventive Medicine and The Environmental Health Center, Jeju National University School of Medicine) ;
  • Her, Kyu-Hee (Department of Surgery, Jeju National University School of Medicine) ;
  • Kim, Heung-Up (Department Internal Medicine, Jeju National University School of Medicine) ;
  • Lee, Jaechun (Department Internal Medicine, Jeju National University School of Medicine) ;
  • Lee, Sang Pyo (Department of Internal Medicine, Gachon University Gil Medical Center) ;
  • Chung, Young-Bae (Department Parasitology, Jeju National University School of Medicine)
  • Received : 2013.10.22
  • Accepted : 2013.12.17
  • Published : 2014.02.28
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Abstract

Several studies have reported that the citrus red mites Panonychus citri were an important allergen of citrus-cultivating farmers in Jeju Island. The aim of the present study was to purify and assess properties of a cysteine protease from the mites acting as a potentially pathogenic factor to citrus-cultivating farmers. A cysteine protease was purified using column chromatography of Mono Q anion exchanger and Superdex 200 HR gel filtration. It was estimated to be 46 kDa by gel filtration column chromatography and consisted of 2 polypeptides, at least. Cysteine protease inhibitors, such as trans poxy-succinyl-L-leucyl-amido (4-guanidino) butane (E-64) and iodoacetic acid (IAA) totally inhibited the enzyme activities, whereas serine or metalloprotease inhibitors did not affect the activities. In addition, the purified enzyme degraded human IgG, collagen, and fibronectin, but not egg albumin. From these results, the cysteine protease of the mites might be involved in the pathogenesis such as tissue destruction and penetration instead of nutrient digestion.

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References

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