DOI QR코드

DOI QR Code

Effects of Peucedanum japonicum Thunb. on Lipid Metabolism and Antioxidative Activities in Rats Fed a High-Fat/High-Cholesterol Diet

갯기름나물이 고지방-고콜레스테롤 식이를 급여한 흰쥐의 지질대사 및 항산화 활성에 미치는 영향

  • 손희경 (조선대학교 식품영양학과) ;
  • 강수태 (조선대학교 식품영양학과) ;
  • 이재준 (조선대학교 식품영양학과)
  • Received : 2014.01.21
  • Accepted : 2014.03.15
  • Published : 2014.05.31

Abstract

This study was carried out to investigate the effects of Peucedanum japonicum Thunb. (PJT) powder on lipid metabolism and antioxidative activity in rats fed a high-fat/high-cholesterol diet for 4 weeks. Male Sprague-Dawley rats were assigned to four groups: normal diet group (N), high-fat/high-cholesterol group (HFC), high-fat/highcholesterol with 5% PJT powder (HFC-PBL), and high-fat/high-cholesterol with 10% PJT powder (HFC-PBH). Body weight gain and food efficiency ratio (FER) increased in the HFC group, whereas they gradually decreased in the PJT powder-fed groups. Food intake was not significantly different between the experimental groups. Liver and adipose tissue weights of the HFC group were heavier than that of the N group, whereas the groups fed PJT powder showed gradual reduction of tissue weights. Serum alkaline phosphatase (ALP) activity significantly decreased after PJT powder administration. Serum triglyceride level significantly decreased in groups fed PJT powder compared to the HFC group. The serum low density lipoprotein cholesterol (LDL-cholesterol) level of the HFC group increased by 73.70% than that of the N group, whereas serum high density lipoprotein (HDL-cholesterol) tended to decrease in groups fed PJT powder compared to the HFC group. Levels of triglycerides in epididymal and mesenteric adipose tissues, as well as cholesterol levels in liver and adipose tissues were lower in the groups fed 10% PJT powder compared to the HFC group. The liver glutathione (GSH) level increased in the groups fed PJT powder compare to the HFC group. The liver thiobarbituric acid reactive substances (TBARS) content significantly decreased in the groups fed PJT powder compared to the HFC group. These results suggest that PJT powder may improve lipid metabolism in the serum, liver, and adipose tissue and prevent oxidative stress by stimulating antioxidative systems in rats fed a high fat-high cholesterol diet.

본 연구는 갯기름나물의 혈청, 간 및 지방조직의 체내 지질대사 개선 및 항산화 효능을 살펴보기 위하여 5주령 된 흰쥐수컷 32마리를 1주일간 적응시킨 후 정상식이군(N), 고지방-고콜레스테롤 식이군(대조군, HFC), 고지방-고콜레스테롤 식이와 5% 갯기름나물 분말 첨가군(HFC-PBL) 및 고지방-고콜레스테롤 식이와 10% 갯기름나물 분말 첨가군(HFC-PBH)으로 나누어 4주간 실시하였다. 체중증가량 및 식이효율은 고지방-고콜레스테롤 식이를 급여한 대조군이 정상식이군에 비하여 유의적으로 증가하였으며, 갯기름나물 분말 첨가군들(HFC-PBL, HFC-PBH)은 대조군에 비하여 유의적으로 감소하였다. 식이섭취량은 대조군이 가장 낮았으나 실험군들 간의 유의차는 나타나지 않았다. 고지방-고콜레스테롤 식이로 증가되어진 간 및 지방조직의 무게는 갯기름나물 분말 첨가로 대조군에 비해 감소하는 경향이었으며, 장간막지방조직 무게는 유의하게 감소하여 정상식이군과 비슷한 경향을 보였다. 갯기름나물 분말 첨가군들의 혈청 중 ALT 및 AST 활성은 대조군에 비하여 유의차는 없었으나 감소하는 경향이었으며, 혈청 중 ALP 활성은 유의적으로 감소하는 경향이었다. 혈청 중 LDH 활성은 갯기름나물 분말 고농도 첨가군(HFC-PBH)은 대조군에 비하여 유의적으로 감소되었다. 혈청 중 중성지방 함량은 갯기름나물 분말 첨가군들이 대조군에 비하여 유의적으로 감소하였고, 혈청 중 총콜레스테롤 함량은 유의차는 없었으나 다소 감소하는 경향이었다. 혈청 중 LDL-콜레스테롤 함량은 정상식이군에 비하여 대조군이 유의하게 증가하였고, 데친 갯기름나물 분말 고농도 첨가군에서 유의한 감소효과를 보였다. 고지방-고콜레스테롤 식이로 증가되어진 간과 지방조직의 중성지방 및 총콜레스테롤 함량은 갯기름나물 분말 첨가로 감소하는 경향이었으며, 부고환지방조직과 장간막지방조직의 중성지방 함량, 간조직과 지방조직 중 총콜레스테롤 함량은 데친 갯기름나물 분말 고농도 첨가군에서 대조군에 비해 유의하게 감소하여 정상식이군과 비슷한 경향이었다. 고지방-고콜레스테롤 식이로 감소되어진 간조직 중 GSH 함량은 갯기름나물 분말 첨가로 유의하게 증가하였으며 증가된 과산화지질 함량은 유의하게 감소하였다. 이상의 실험결과 in vivo에서 고지방-고콜레스테롤 식이와 함께 섭취한 갯기름나물 분말은 체중, 간 및 지방조직의 무게 감소와 더불어 혈청 및 지방조직의 지질대사 개선에 긍정적인 영향을 미치는 것으로 나타났다. 이러한 결과는 갯기름나물에 함유된 항산화 비타민 및 무기질, polyphenol, flavonoid 등 생리활성 물질에 의해 지방조직의 체지방 형성을 억제하고 산화적 스트레스를 완화시킴으로써 체내 지질대사 개선과 심혈관계 질환 예방효과를 나타낼 수 있을 것으로 생각된다.

Keywords

References

  1. Byun JH. 1995. The goals and strategy for health promotion. Korean Institute for Health and Social Affairs, Seoul, Korea. p 225-229.
  2. Lee HK. 1996. Korean disease pattern and nutrition. Korean J Nutr 29: 381-383.
  3. National Statistical Office Republic of Korea. 2011. Annual report on the cause of death statistics. Statistics Korea, Seoul, Korea.
  4. Nordmann R, Ribiere C, Rouach H. 1990. Ethanol-induced lipid peroxidation and oxidative stress in extrahepatic tissues. Alcohol Alcohol 25: 231-237.
  5. Bergmeyer HU. 1974. Methods of enzymatic analysis. 2nd ed. Academic press, New York, NY, USA. p 860-864.
  6. Kim HJ, Hwang Bo MH, Lee JW, Im HG, Lee IS. 2007. Antioxidant effects of ginseng powder on liver of benzo (${\alpha}$)pyrene-treated mice. Korean J Food Sci Technol 39: 217-221.
  7. Lovlin R, Cottle W, Pyke I, Kavanagh M, Belcastro AN. 1987. Are indices of free radical damage related to exercise intensity. Eur J Appl Physiol Occup Physiol 56: 313-316. https://doi.org/10.1007/BF00690898
  8. Halliwell B. 1996. Antioxidants in human health and disease. Annu Rev Nutr 16: 33-50. https://doi.org/10.1146/annurev.nu.16.070196.000341
  9. Yokozawa T, Nakagawa T, Kitani K. 2002. Antioxidative activity of green tea polyphenol in cholesterol-fed rats. J Agric Food Chem 50: 3549-3552. https://doi.org/10.1021/jf020029h
  10. Cross CE, Halliwell B, Borish ET, Pryor WA, Ames BN, Saul RL, McCord JM, Harman D. 1987. Oxygen radicals and human disease. Ann Intern Med 107: 526-545. https://doi.org/10.7326/0003-4819-107-4-526
  11. Liu RH. 2003. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr 78: 517S-520S.
  12. West IC. 2000. Radicals and oxidative stress in diabetes. Diabetes Med 17: 171-180. https://doi.org/10.1046/j.1464-5491.2000.00259.x
  13. Nam JY, Pyu KS. 1975. Pharmacogenetics studies on Korean "Bang Poong". Kor J Pharmacogn 6: 151-159.
  14. Moon KS, Choi OJ. 1991. Composition and use of medicinal herbs. Ilwolbooks, Seoul, Korea. p 449-450.
  15. Kim DH, Han CS, Kim GE, Kim JH, Kim SG, Kim HK, Oh OJ, Whang WK. 2009. Biological activities of isolated compounds from Peucedani radix. Yakhak Hoeji 53: 130-137.
  16. Shin KH, Kang SS, Chi HJ. 1992. Analysis of the coumarin constituents in Peucedanii radix. Kor J Pharmacogn 23: 20-23.
  17. Cho YH, Seong NS, Ham IH, Choi HY. 2004. A comparative study on the immunization and anti-cancer effect of the root and the aerial part of Peucedanum japonicum Thunb. Kor J Herbology 19: 137-145.
  18. Gyeongsangbuk-do Agricultural Research & Extension Services. 2006. Antifungal which the falcarindiol isolated from Peucedanum japonicum is used. Korean Patent 1020000083118.
  19. Shim WS. 2013. Makguksu containing Peucedanum japonicum Thunberg and method for manufacturing the same. Korean Patent 1020110075622.
  20. Reeves PG, Nielson FH, Fahey Jr GC. 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123: 1939-1951.
  21. Friedwald W, Levy R, Fredrickson D. 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18: 499-502.
  22. Folch J, Lees M, Sloane Stanley GH. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497-509.
  23. Zlatkis A, Zak B. 1969. Study of a new cholesterol reagent. Anal Biochem 29: 143-148. https://doi.org/10.1016/0003-2697(69)90017-7
  24. Biggs HG, Erikson JM, Moorehead WR. 1975. A manual colorimetric assay of triglyceride in serum. Clin Chem 21: 437-441.
  25. Tietze F. 1969. Enzymatic methods for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 27: 502-522. https://doi.org/10.1016/0003-2697(69)90064-5
  26. Buege JA, Aust SD. 1978. The thiobarbituric acid assay. Methods in Enzymol 5: 306-307.
  27. Lee JJ, Choo MH, Lee MY. 2006. Effect of Pimpinella brachycarpa extract on lipid metabolism in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 36: 1151-1158. https://doi.org/10.3746/jkfn.2006.35.9.1151
  28. Choi MY, Choi EJ, Lee E, Park HJ. 2000. Effect of Oenanthe javanica sap on plasma lipid composition in rats with high-fat diet. Korean J Plant Res 13: 54-60.
  29. Son HK. 2013. Effects of Peucedanum japonicum Thunb. powder on lipid metabolism and antioxidative activities in rats fed a high-fat/high-cholesterol diet. MS Thesis. Chosun University, Gwangju, Korea.
  30. Chai YM, Lim BK, Lee JY, Kim YH, Rhee SJ. 2003. Preparation of soluble dietary fiber from oak wood (Quercus mongolica) and its physiological function in rat fed high cholesterol diets. Korean J Nutr 36: 9-17.
  31. Jayasooriya AP, Sakono M, Yukizaki C, Kawano M, Yamamoto K, Fukuda N. 2000. Effects of Momordica charantia powder on serum glucose levels and various lipid parameters in rats fed with cholesterol-free and cholesterol-enriched diets. J Ethnopharmacol 72: 331-336. https://doi.org/10.1016/S0378-8741(00)00259-2
  32. Wursch P. 1979. Influence of tannin-rich carob pod fiber on the cholesterol metabolism in the rat. J Nutr 109: 685-692.
  33. Park JR, Park SK, Cho YS, Chun SS, Choi SH, Park JC. 1997. Effects of Angelica keiskei on lipid metabolism in rats. J Korean Soc Food Sci Nutr 26: 308-313.
  34. Bjorntorp P. 1988. The associations between obesity, adipose tissue distribution and disease. Acta Med Scand Suppl 723: 121-134.
  35. Despres JP. 1993. Abdominal obesity as important component of insulin-resistant syndrome. Nutrition 19: 452-459.
  36. Kwon MJ, Nam TJ. 2006. Effects of Mesangi (Capsosiphon fulvecens) powder on lipid metabolism in high cholesterol fed rats. J Korean Soc Food Sci Nutr 35: 530-535. https://doi.org/10.3746/jkfn.2006.35.5.530
  37. Plaa GL, Charbonneau M. 1994. Detection and evaluation of chemically induced liver injury. In Principles and Methods of Toxicology. Hayes AW, ed. Raven Press, New York, NY, USA. p 839-870.
  38. Heo YY, Ha BJ. 2011. Effect of Ligusticum chuonxiong Hort extracts on the bioactivity in high-fat diet-fed obese rats. J Fd Hyg Safety 26: 370-376.
  39. Jang JY, Lee MK, Kim MJ, Cho SY. 1998. Effect of fiber on serum lipid metabolism in rats with diet-induced cholesterolemia. J Korean Soc Food Sci Nutr 27: 1211-1216.
  40. Buhman KK, Furumoto EJ, Donkin SS, Story JA. 1998. Dietary psyllium increases fecal bile acid excretion, total steroid excretion and bile acid biosynthesis in rats. J Nutr 128: 1199-1203.
  41. Kang HJ, Song YS. 1997. Dietary fiber and cholesterol metabolism. J Korean Soc Food Sci Nutr 26: 358-369.
  42. Kang JA, Kang JS. 1997. Effects of garlic and onion on plasma an liver cholesterol and triglyceride and platelet aggregation in rate basal or cholesterol supplemented diets. Korean J Nutr 32: 132-138.
  43. Vahouny GV, Roy T, Gallo LL, Story JA, Kritchevsky D, Cassidy M. 1980. Dietary fibers. III. Effects of chronic intake on cholesterol absorption and metabolism in the rat. Am J Clin Nutr 33: 2182-2191.
  44. Nam JH, Park HS. 1993. Effect of quality and quantity of dietary fat on status of tocopherol and lipid peroxidation of plasma tissue in rats. Korean J Nutr 26: 566-577.
  45. Lee JS, Lee KH, Jeong JH. 1999. Effects of extract of Pueraria radix on lipid metabolism in rats fed high fat diet. J Korean Soc Food Sci Nutr 28: 218-224.
  46. Joo HY, Lim KT. 2009. Protective effect of glycoprotein isolated from Cudrania tricuspidata on liver in CCl$_{4}$-treated A/J mice. Korean J Food Sci Technol 41: 93-99.
  47. Kim YN, Ku KH, Kwon KS, Hwa CJ. 2011. Effects of water extracts of red pepper seeds powder on antioxidative enzyme activities and oxidative damage in rats fed high-fat and high-cholesterol diets. Korean J Nutr 44: 284-291. https://doi.org/10.4163/kjn.2011.44.4.284

Cited by

  1. Occurrence of Rust on Peucedanum japonicum Caused by Puccinia jogashimensis in Korea vol.21, pp.4, 2015, https://doi.org/10.5423/RPD.2015.21.4.337
  2. Nutritional quality of Peucedanum japonicum Thunb. leaves in relation to ripening time, growing condition and blanching vol.21, pp.6, 2014, https://doi.org/10.11002/kjfp.2014.21.6.784
  3. Effects of Hibiscus syriacus Extracts on Antioxidant Activities and Blood Circulation Improvement vol.26, pp.12, 2016, https://doi.org/10.5352/JLS.2016.26.12.1415
  4. Effects of Dietary Resveratrol on Growth Performance, Blood Biochemical Parameter, Immunoglobulin, and Blood Antioxidant Activity in Broiler Chicks vol.42, pp.2, 2015, https://doi.org/10.5536/KJPS.2015.42.2.147
  5. Quality Characteristics and Antioxidant Activities of Cookies Added with Peuceddanum japonicom Thunb Powder vol.25, pp.5, 2016, https://doi.org/10.5934/kjhe.2016.25.5.595
  6. Effects of Salvia plebeia R. Br. on Antioxidative Enzyme Activities and Oxidative Damage in Rats Fed High-Fat and High-Cholesterol Diets vol.33, pp.4, 2018, https://doi.org/10.13103/JFHS.2018.33.4.316
  7. First Report of Wilt Disease on Peucedanum japonicum and Selection of Fungicides vol.24, pp.1, 2014, https://doi.org/10.7585/kjps.2020.24.1.7
  8. Potential Application of Cornelian Cherry Extract on Broiler Chickens: Growth, Expression of Antioxidant Biomarker and Glucose Transport Genes, and Oxidative Stability of Frozen Meat vol.11, pp.4, 2014, https://doi.org/10.3390/ani11041038