Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Hypopigmenting Effects of Extracts from Bulbs of Lilium Oriental Hybrid 'Siberia' in Murine B16/F10 Melanoma Cells

백합뿌리 추출물의 멜라닌 생성 억제효과

  • Yoon, Hoon Seok (Cosmetic Science Center, Dept. of Chemistry, Jeju National University) ;
  • Yang, Kyung-Wol (Jeju Love Co., Ltd.) ;
  • Kim, Jung Eun (Cosmetic Science Center, Dept. of Chemistry, Jeju National University) ;
  • Kim, Jeong Mi (Jeju Love Co., Ltd.) ;
  • Lee, Nam Ho (Cosmetic Science Center, Dept. of Chemistry, Jeju National University) ;
  • Hyun, Chang-Gu (Cosmetic Science Center, Dept. of Chemistry, Jeju National University)
  • 윤훈석 (제주대학교 화장품과학연구센터) ;
  • 양경월 ((주)제주사랑) ;
  • 김정은 (제주대학교 화장품과학연구센터) ;
  • 김정미 ((주)제주사랑) ;
  • 이남호 (제주대학교 화장품과학연구센터) ;
  • 현창구 (제주대학교 화장품과학연구센터)
  • Received : 2014.01.22
  • Accepted : 2014.04.01
  • Published : 2014.05.31
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Abstract

In order to develop a skin-whitening agent, melanin contents and intracellular tyrosinase activity were determined by western blotting. Ethyl acetate fractions of 80% ethanol extracts from lily (Lilium Oriental Hybrid 'Siberia') bulbs (R-EA) inhibited melanin synthesis in a dose-dependent manner in ${\alpha}$-melanocyte stimulating hormone (${\alpha}$-MSH)-treated B16/F10 murine melanoma cells. Intracellular tyrosinase activity and melanin contents were suppressed by 45% and 74%, respectively, in response to treatment with $100{\mu}g/mL$ of R-EA. Examination of protein expression associated with ${\alpha}$-MSH-induced melanogenesis showed that tyrosinase related protein (TRP)-1 was inhibited more strongly than tyrosinase, and these results were correlated with stronger inhibition of melanin synthesis than intracellular tyrosinase activity. Taken together, R-EA containing p-coumaric acid and resveratrol could be used as a hypopigmentation agent through suppression of sustained extracellular signal-regulated kinase (ERK) activation via melanogenic induction.

피부미백 소재를 개발하기 위해 멜라닌 함량, 세포내 tyrosinase 활성의 측정 및 Western blotting 실험이 수행되었다. 백합(Lilium Oriental Hybrid 'Siberia') 뿌리의 80% 에탄올 추출물로부터 얻은 에틸아세테이트 분획물(R-EA)은 ${\alpha}$-melanocyte stimulating hormone(${\alpha}$-MSH)에 의해 멜라닌 생성이 유도된 B16/F10 흑색종 세포에서 농도 의존적으로 멜라닌 생성을 저해하였다. 정확하게 세포내 tyrosinase 활성과 멜라닌 함량은 에틸아세데이트 분획물 $100{\mu}g/mL$ 처리 시 ${\alpha}$-MSH 단독 처리군에 비해 각각 45%와 74%의 저해율을 보였다. ${\alpha}$-MSH에 의해 멜라닌 생성이 유도된 B16/F10 흑색종 세포에서 단백질 발현양상을 살펴본 결과 TRP-1이 가장 많이 억제된 양상을 확인할 수 있었고 이 결과는 세포내 tyrosinase 활성저해보다 멜라닌 생성저해가 더 많이 일어난 것과 일맥상통하는 것이다. 이를 종합해 볼 때 p-coumaric acid와 resveratrol 함량이 백합뿌리의 에탄올 추출물에 비해 많이 함유된 에틸아세테이트 분획물은 멜라닌 생성 유도물질에 의해 촉진된 ERK의 활성화를 억제하는 피부미백 소재로서 그 가치가 입증된다고 사료된다.

Keywords

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