Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Antioxidant Ability and Hair Growth Effect of Samhwang-Sasimtang in C57BL/6 Model

삼황사심탕의 항산화능 및 C57BL/6 마우스 모델에서의 발모 촉진효과

  • Kim, Min Ji (Department of Public Health, College of Natural Sciences, Keimyung University) ;
  • Park, Kyo Hyun (Department of Public Health, College of Natural Sciences, Keimyung University) ;
  • Lee, In Chul (Department of Cosmetic Science and Technology, Seowon University) ;
  • Kim, Bae Hwan (Department of Public Health, College of Natural Sciences, Keimyung University)
  • 김민지 (계명대학교 자연과학대학 공중보건학과) ;
  • 박교현 (계명대학교 자연과학대학 공중보건학과) ;
  • 이인철 (서원대학교 화장품과학과) ;
  • 김배환 (계명대학교 자연과학대학 공중보건학과)
  • Received : 2014.02.23
  • Accepted : 2014.03.31
  • Published : 2014.04.25
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Abstract

The purpose of this study is to evaluate the antioxidant activity and hair growth effects of a natural herbal ethanol extract, Samhwang-Sasimtang(SS). In case of antioxidant ability of SS, the content of phenolic compounds was 28.44mg/g. The extract showed strong electron donating ability and free radical scavenging activity in a concentration-dependent manner. SOD-like activity also rose through increasing the concentrations of SS. In order to estimate the hair growth effects, the extract was applied to the back of seven-week-old C57BL/6 male mice ($150{\mu}{\ell}$ a day, five days a week, for four weeks) in four groups (C, control, saline; PC, positive control, 3% minoxidil; E1, experimental 1, 1% SS; E2, experimental 2, 2% SS). Ten mice were assigned to each group and five mice in each group were sacrificed at weeks 2 and 4, respectively. There was no a significant difference in body weight change among experimental groups. In macroscopic observation of hair growth at week 4, the scores of hair growth on the backs of mice were 80, 60, 40 and 20% in the PC, E2, E1 and C groups. In terms of histological observation, the ratio and thickness of hair follicles, the enzyme activities of ALP and ${\gamma}$-GT, immunohistological examination of IGF-1 and VEGF which are the hair growth factors, in each group were significantly high in this order: PC, E2, E1 and C group, at week 4. Meanwhile, hair growth inhibition factors, TGF-${\beta}1$ and Caspase-3, were reduced in PC, E1 and E2 groups compared with C group. These results indicate that SS extract may be effective in promoting hair growth, and suggest that it can be used practically as a superior natural agent for hair growth promotion.

Keywords

References

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