Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Persimmon leaf on the Photoaging Skin Improvement(2)

감잎의 광노화 피부 개선에 미치는 효과(2)

  • Lee, Chang Hyun (College of Korean Medicine, Woosuk University) ;
  • Kim, Nam Seok (Department of Food & Biotechnology, Woosuk University) ;
  • Choi, Dong Seong (Department of Food & Biotechnology, Woosuk University) ;
  • Oh, Mi Jin (Department of Food & Biotechnology, Woosuk University) ;
  • Ma, Sang Yong (Department of Alternative Medicine, Woosuk University) ;
  • Kim, Myoung Soon (Department of Alternative Medicine, Woosuk University) ;
  • Ryu, Seung Jeong (Dongsangmyunsaram Farming Cooperations) ;
  • Kwon, Jin (Department of Prosthetics and Orthotics, Korea National College of Welfare) ;
  • Shin, Hyun Jong (College of Korean Medicine, Woosuk University) ;
  • Oh, Chan Ho (Department of Food & Biotechnology, Woosuk University)
  • 이창현 (우석대학교 한의과대학) ;
  • 김남석 (우석대학교 식품생명공학과) ;
  • 최동성 (우석대학교 식품생명공학과) ;
  • 오미진 (우석대학교 식품생명공학과) ;
  • 마상용 (우석대학교 대체의학과) ;
  • 김명순 (우석대학교 대체의학과) ;
  • 유승정 (동상면사람들) ;
  • 권진 (한국복지대학교 의료보장구과) ;
  • 신현종 (우석대학교 한의과대학) ;
  • 오찬호 (우석대학교 식품생명공학과)
  • Received : 2013.12.02
  • Accepted : 2013.12.29
  • Published : 2014.02.25
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Abstract

This study was performed to investigate the anti-photoaging effects of Persimmon leaf tea(PLT) in hairless mice(SKH-1) exposed to UVB radiation. The animals were divided into non-treated group (normal, N) and UV-radiated groups. UV-radiated groups were divided into only UV-radiated group(control, C) and UV-radiated and PLT treated experimental groups[first extraction treated group(PLT-I), second extraction treated groupe(PLT-II), and third extraction treated group(PLT-III)]. Three PLT treated experimental groups of mice were treated with both oral administration(300mg/Kg B.W./day) and topical application (100 ul of 2% conc./mouse/day) for 4 weeks. Anti-photoaging effects of Persimmon leaf were evaluated by MTT assay, anti oxidative reaction, MMP immunohistochemistry, gelatin zymography assay and RT-PCR observations. Treatment with Persimmon leaf tea(PLT)-I, and -III groups decreased immunohistochemical density of matrix metalloproteinases(MMP)-3 and -9 related to degradation of extracellular matrix in skin. Especially, immunohistochemical density of MMP-2 decreased in PLT-I, -II and -III groups in skin. On the effects of antioxidant function on the treatment with Persimmon leaf tea(PLT), treatment of HaCaT cells with extracts of PLT-I and PLT-II had also significantly reduced intracellular ROS produced by UVB irradiation in a dose dependent manner(PLT-I, p<0.05, p<0.01, p<0.001; PLT-II, p<0.01, p<0.001). Gelatin zymography assay revealed that PLT-II and PLT-III (200 ug/ml) had inhibitory effect on MMP-9 expression in UVB-radiated HaCaT cells. Western blot analysis revealed that PLT-1, -II and -III groups down-regulates the expression of inflammatory associated genes(IL-$1{\beta}$) and PLT-1 and -II groups down-regulates the expression of TNF-${\alpha}$ in a dose dependent manner. Our study suggests that Persimmon leaf tea(PLT) extracts participates in inhibitory effects on the morphological and molecular experiments related to photoaging skin on UVB irradiated hairless mice.

Keywords

References

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