Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Methanol Extract of Alpinia katsumadai Hayata has Anti-viral Activity by Inhibition of α-glucosidase

초두구 추출물의 α-glucosidase 활성 억제에 의한 항바이러스 활성

  • Lee, Doseung (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark) ;
  • Boo, Kyung Hwan (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kim, Young Cheon (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Lee, Jin-Man (Department of Food & Biotechnology, Hoseo University) ;
  • Kim, Seong Cheol (Agricultural Research Center for Climate Change, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Wang Shik (College of Applied Life Science(SARI), Jeju National University, Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University) ;
  • Riu, Key Zung (College of Applied Life Science(SARI), Jeju National University, Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University) ;
  • Lee, Dong-Sun (College of Applied Life Science(SARI), Jeju National University, Research Institute for Subtropical Agriculture and Biotechnology, Jeju National University)
  • Received : 2013.11.26
  • Accepted : 2013.12.16
  • Published : 2014.06.30
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Abstract

In the present study, we investigated the effects of methanol extracts from Alpinia katsumadai Hayata against antiviral potential underlying mechanism in glucosidase inhibition. Syncytium formation in Newcastle disease virus (NDV)-infected baby hamster kidney (BHK) cell originates from the trafficking of viral glycoprotein into cell-surface. Methanol extracts inhibited not only syncytium formation, but also trafficking of glycoprotein, hemagglutinin-neuraminidase (HN), onto cell-surface. A. katsumadai extracts showed the inhibitory activities ($IC_{50}$ $25{\mu}g/mL$) against ${\alpha}$-glucosidase. These results suggested that blue chanterelle extracts inhibited the cell-surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.

${\alpha}$-glucosidase 활성을 저해하는 초두구 메탄올 추출물의 항바이러스 활성을 연구하였다. Newcastle disease virus (NDV) 감염된 baby hamster kidney (BHK) 세포에서 Syncytium (합포체) 형성은 세포막 표면으로의 수송된 바이러스 당단백질 hemagglutinin-neuramidase (HN)에 의해 일어난다. 초두구 추출물$IC_{50}$ $25{\mu}g/mL$) 처리된 세포에서 바이러스 당단백질의 수송과 함께 합포체 형성이 저해되었다. 또한 $IC_{50}$ 농도에서는 세포 내에서 당단백질 생합성은 저해되지 않으며 당단백질의 수송을 저해하는 것으로 추론된다.

Keywords

References

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