Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Protective Effects of Radiation-induced Blackberry Mutant Extract on Carbon Tetrachloride (CCl4)-induced Liver Injury in Sprague-Dawley Rats

방사선 돌연변이 블랙베리 추출물의 사염화탄소(Carbon Tetrachloride, CCl4) 투여에 의한 흰쥐의 간 손상에 대한 보호 효과

  • Cho, Byoung Ok (Advanced Radiation Technology Institute, Korea Atomic & Energy Research Institute) ;
  • Lee, Chang-Wook (Advanced Radiation Technology Institute, Korea Atomic & Energy Research Institute) ;
  • So, Yangkang (Advanced Radiation Technology Institute, Korea Atomic & Energy Research Institute) ;
  • Jin, Chang-Hyun (Advanced Radiation Technology Institute, Korea Atomic & Energy Research Institute) ;
  • Yook, Hong-Sun (Dept. of Food and Nutrition, Chungnam National University) ;
  • Byun, Myung-Woo (Dept. of Culinary Nutrition, Woosong University) ;
  • Jeong, Yong-Wook (Dept. of Microbiology, College of Medicine, Seonam University) ;
  • Park, Jong Chun (Dept. of Microbiology, College of Medicine, Seonam University) ;
  • Jeong, Il-Yun (Advanced Radiation Technology Institute, Korea Atomic & Energy Research Institute)
  • 조병옥 (한국원자력연구원 첨단방사선연구소) ;
  • 이창욱 (한국원자력연구원 첨단방사선연구소) ;
  • 소양강 (한국원자력연구원 첨단방사선연구소) ;
  • 진창현 (한국원자력연구원 첨단방사선연구소) ;
  • 육홍선 (충남대학교 식품영양학과) ;
  • 변명우 (우송대학교 외식조리영양학부) ;
  • 정용욱 (서남대학교 의과대학 미생물학교실) ;
  • 박종천 (서남대학교 의과대학 미생물학교실) ;
  • 정일윤 (한국원자력연구원 첨단방사선연구소)
  • Received : 2014.02.05
  • Accepted : 2014.04.08
  • Published : 2014.06.30
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Abstract

The objective of the present study was to investigate the protective effects of anthocyanin-enriched extract from radiation-induced blackberry (Rubus fruticosus L.) mutant (${\gamma}$-B201) against carbon tetrachloride ($CCl_4$)-induced liver injury in Spargue-Dawley (SD) rats. The in vivo results show that ${\gamma}$-B201 attenuated the levels of serum aspartate aminotransferase, alanine aminotransferase, and liver lipid peroxidation in $CCl_4$-treated SD rats. Histopathological examination of rat livers showed that ${\gamma}$-B201 reduced the incidence of liver lesions induced by $CCl_4$. Moreover, ${\gamma}$-B201 prevented DNA damage in $CCl_4$-treated SD rats. Furthermore, administration of ${\gamma}$-B201 significantly increased the activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), in $CCl_4$-treated rat livers. In conclusion, the present study suggests that ${\gamma}$-B201 blackberry extract protects the liver from $CCl_4$-induced hepatic damage through an antioxidant mechanism. Therefore, ${\gamma}$-B201 blackberry may be functional food material for human health.

본 연구에서는 흰쥐 SD rats에서 $CCl_4$로 유도된 간 손상에 미치는 방사선 돌연변이 블랙베리 ${\gamma}$-B201 추출물의 간 기능 개선 효과에 대하여 알아보고자 하였다. ${\gamma}$-B201 추출물을 경구 투여 시 SD rats에 $CCl_4$ 처리로 인해 증가된 혈청 AST, ALT 농도를 억제함을 확인하였다. 나아가 지질과산화 대사산물인 MDA의 농도를 억제함을 확인하였고, 조직염색 관찰 결과 손상된 간 조직을 ${\gamma}$-B201 추출물에 의해 회복되는 것을 확인하였다. Comet assay를 통해 DNA 손상정도를 분석한 결과, $CCl_4$로 인해 유발된 산화적 스트레스에 의한 DNA 손상을 ${\gamma}$-B201 추출물의 경구 투여로 인하여 유의적으로 감소시켰다. $CCl_4$ 처리로 인해 감소된 SOD, CAT, GPx, GR의 항산화 효소 활성이 증가함을 확인하였다. 이러한 결과로 볼 때 방사선 돌연변이 ${\gamma}$-B201 추출물은 항산화 효소 활성을 증가시킴으로써 $CCl_4$에 의해 손상된 SD rat의 간 조직을 보호하는 것으로 판단되며, 방사선 돌연변이 ${\gamma}$-B201은 간 손상 보호 및 간 기능 개선 효과를 갖는 기능성 소재로 활용되어질 수 있을 것으로 사료된다.

Keywords

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