Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antiadipogenic Effect of Korean Glasswort (Salicornia herbacea L.) Water Extract on 3T3-L1 Adipocytes

세포배양 모델을 통한 함초(Salicornia herbacea L.) 물 추출물의 항비만 효과 탐색

  • Kim, Mi Joung (Dept. of Food and Nutrition, College of Natural Sciences, Seoul Women's University) ;
  • Jun, Hyun Young (Dept. of Food and Nutrition, College of Natural Sciences, Seoul Women's University) ;
  • Kim, Jung Hee (Dept. of Food and Nutrition, College of Natural Sciences, Seoul Women's University)
  • 김미정 (서울여자대학교 자연과학대학 식품영양학과) ;
  • 전현영 (서울여자대학교 자연과학대학 식품영양학과) ;
  • 김정희 (서울여자대학교 자연과학대학 식품영양학과)
  • Received : 2014.02.11
  • Accepted : 2014.03.29
  • Published : 2014.06.30
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Abstract

As a natural plant ingredients, glasswort (Salicornia herbacea L.) contains various physiological activities, mainly anti-oxidative and anti-diabetic activities. However, only a few studies have been carried out on its anti-adipogenic effect. This study investigated the anti-obesity effects of Salicornia herbacea L. on 3T3-L1 adipocytes. As adipogenesis of preadipocytes to adipocytes involves proliferation and differentiation of cells, we treated three concentrations (125, 250, and $500{\mu}g/mL$) of Salicornia herbacea L. water extracts (SLW) in both pre-processing and post-processing stages. When 3T3-L1 adipocytes were differentiated and dyed with Oil Red O, adipocytes size and the value of relative Oil Red O staining were reduced by all concentrations of SLW in post-processing stage. Following adipogenic differentiation, the concentration of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in the cell supernatant significantly increased upon treatment with $125{\mu}g/mL$ of SLW and further rose at concentrations of 250 and $500{\mu}g/mL$ during post-processing stage. There was no significant difference in glycerol production upon SLW treatment. Leptin production significantly decreased at all SLW concentrations during post-processing stage, whereas peroxisome proliferator activated receptor-${\gamma}$ (PPAR-${\gamma}$) and adiponectin secretions were significantly enhanced. Overall results showed that SLW might have an anti-adipogenic effect via enhancement of TNF-${\alpha}$ production, which causes dedifferentiation and inhibits lipid accumulations in adipocyte. Furthermore, SLW might prevent diabetes and cardiovascular disease, as it reduces leptin secretion and enhances production of both PPAR-${\gamma}$ and adiponectin. However, further research is needed to elucidate the exact mechanism and bioactive compounds of glasswort.

본 연구에서는 염생식물의 하나인 한국산 함초(Salicornia herbacea L.) 물 추출물의 항비만 효과를 알아보고자 지방세포를 이용하여 실험을 실시하였다. 3T3-L1 adipocytes 세포의 지방분화 억제 효과를 살펴보기 위해 함초 물 추출물을 농도별(125, 250, $500{\mu}g/mL$), 처리시기별(전처리, 후처리)로 처리한 결과, 함초 물 추출물을 후처리 하였을 때 지방세포의 크기와 지방축적 정도가 감소하였다. 또한 함초물 추출물 후 처리 시 $125{\mu}g/mL$ 농도에서 세포로부터의 TNF-${\alpha}$의 생성이 유의하게 증가하였고, 세포로부터 분비된 glycerol 양에는 영향을 미치지 않았다. 이러한 결과는 함초물 추출물이 지방분화 유도 후 지방이 축적되는 과정에서 지방분해보다는 지방합성 과정에 관련하여 지방세포 분화를 억제하는 효과가 있는 것으로 여겨진다. 함초 물 추출물 후처리 시 지방축적 감소로 인해 지방세포에서 분비되는 인슐린 저항성과 관련된 leptin 및 resistin 생성은 감소하였고, 인슐린 민감성을 증가시키고 지방세포로의 에너지원 유입을 증가시키는 PPAR-${\gamma}$ 및 이로부터 발현이 유도되어 심혈관계 질환 예방에 관련된 adiponectin 생성을 증가시켜 인슐린 민감성과 관련된 당뇨병 및 심혈관계 질환 예방 효과를 가질 가능성이 있을 것으로 나타났다. 그러나 정확한 관련 기전에 대한 더 많은 연구와 함초 물 추출물의 기능성 성분을 밝히는 추후 연구가 필요하다.

Keywords

References

  1. Ministry of Health and Welfare. 2010. Report of 2009 Korean National Health and Nutritional Examination Survey, KNHANES IV. 2009. http://www.mw.go.kr/front_new/jb/sjb030301vw.jsp?PAR_MENU_ID=03&MENU_ID=031604&CONT_SEQ=264574&page=1 (accessed April2011).
  2. Albu J, Allison D, Boozer CN, Heymsfield S, Kissileff H, Kretser A, Krumhar K, Leibel R, Nonas C, Pi-Sunyer X, VanItallie T, Wedral E. 1997. Obesity solutions: report of a meeting. Nutr Res 55: 150-156.
  3. Grundy SM. 1998. Multifactorial causation of obesity: implications for prevention. Am J Clin Nutr 67: 563S-572S.
  4. Aronne LJ. 1998. Modern medical management of obesity:the role of pharmaceutical intervention. J Am Diet Assoc 98: S23-S26. https://doi.org/10.1016/S0002-8223(98)00008-X
  5. Zacour AC, Silva ME, Cecon PR, Bambirra EA, Vieira EC. 1992. Effect of dietary chitin on cholesterol absorption and metabolism in rats. J Nutr Sci Vitaminol (Tokyo) 38: 609-613. https://doi.org/10.3177/jnsv.38.609
  6. Delzenne NM, Cani PD, Daubioul C, Neyrinck AM. 2005. Impact of inulin and oligofructose on gastrointestinal peptides. Br J Nutr 93: S157-S161. https://doi.org/10.1079/BJN20041342
  7. Oh SJ, Kim YS, Park CY, Kim SW, Yang IM, Kim JW, Choi YK, Paeng JR, Shin HD. 2000. Body fat decreasing mechanisms of red ginseng compound. Korean J Obesity 9: 209-218.
  8. Rhee SJ, Kim KR, Kim HT, Hong JH. 2007. Effects of catechin on lipid composition and adipose tissue in obese rats fed high fat diet. J Korean Soc Food Sci Nutr 36: 540-547. https://doi.org/10.3746/jkfn.2007.36.5.540
  9. Jeon JR, Kim JY, Lee KM, Cho DH. 2005. Anti-obese effects of mixture contained pine needle, black tea and green tea extracts. J Korean Soc Appl Biol Chem 48: 375-381.
  10. Kim CS, Song TG. 1983. Ecological studies on the halophyte communities at western and southern coasts in Korea (IV)-The halophyte communities at the different salt marsh habitats. Korean J Ecol 6: 167-176.
  11. Kim KR, Choi JH, Lee SK, Woo MH, Choi SW. 2006. Effect of enzymatic hydrolysate of Hamcho (Salicornia herbacea L.) on antioxidative defense system in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 35: 1356-1362. https://doi.org/10.3746/jkfn.2006.35.10.1356
  12. Do JR, Kim EM, Koo JG, Jo KS. 1997. Dietary fiber contents of marine algae and extraction condition of the fiber. J Korean Fish Soc 30: 291-296.
  13. Jo YC, Ahn JH, Chon SM, Lee KS, Bae TJ, Kang DS. 2002. Studies on pharmacological effects of glasswort (Salicornia herbacea L.). Korean J Medicinal Crop Sci 10: 93-99.
  14. Song HS, Kim DP, Jung YH, Lee MK. 2007. Antioxidant activities of red Hamcho (Salicornia herbacea L.) against lipid peroxidation and the formation of radicals. Korean J Food & Nutr 20: 150-157.
  15. Jung BM, Park JA, Bae SJ. 2008. Growth inhibitory and quinone reductase induction activities of Salicornia herbacea L. fractions on human cancer cell lines in vitro. J Korean Soc Food Sci Nutr 37: 148-153. https://doi.org/10.3746/jkfn.2008.37.2.148
  16. Kong CS, Kim YA, Kim MM, Park JS, Kim JA, Kim SK, Lee BJ, Nam TJ, Seo Y. 2008. Flavonoid glycosides isolated from Salicornia herbacea inhibit matrix metalloproteinase in HT1080 cells. Toxicol In Vitro 22: 1742-1748. https://doi.org/10.1016/j.tiv.2008.07.013
  17. Bang MA, Kim HA, Cho YJ. 2002. Hypoglycemic and antioxidant effect of dietary Hamcho powder in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 31:840-846. https://doi.org/10.3746/jkfn.2002.31.5.840
  18. Kim SH, Ryu DS, Lee MY, Kim KH, Kim YH, Lee DS. 2008. Anti-diabetic activity of polysaccharide from Salicornia herbacea. Kor J Microbiol Biotechnol 36: 43-48.
  19. Vahouny GV, Roy T, Gallo LL, Story JA, Kritchevsky D, Cassidy M. 1980. Dietary fibers. III. Effects of chronic intake on cholesterol absorption and metabolism in the rat. Am J Clin Nutr 33: 2182-2191.
  20. Kuda T, Fujii T, Saheki K, Hasegawa A, Okuzumi M. 1992. Effects of brown algae on faecal flora of rats. Nippon Suisan Gakkaishi 58: 307-314. https://doi.org/10.2331/suisan.58.307
  21. Kim AR, Lee JJ, Lee YM, Jung HO, Lee MY. 2010. Cholesterol- lowering and anti-obesity effects of Polymnia sonchifolia Poepp. & Endl. powder in rats fed a high fat-high cholesterol diet. J Korean Soc Food Sci Nutr 39: 210-218. https://doi.org/10.3746/jkfn.2010.39.2.210
  22. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res 47: 936-942.
  23. Smas CM, Sul HS. 1995. Control of adipocyte differentiation. Biochem J 309: 697-710.
  24. Gregoire FM. 2001. Adipocyte differentiation: from fibroblast to endocrine cell. Exp Biol Med (Maywood) 11: 997-1002.
  25. McNeel RL, Mersmann HJ. 2003. Effects of isomers of conjugated linoleic acid on porcine adipocyte growth and differentiation. J Nutr Biochem 14: 266-274. https://doi.org/10.1016/S0955-2863(03)00031-7
  26. Caro JF, Sinha MK, Kolaczynski JW, Zhang PL, Considine RV. 1996. Leptin: the tale of an obesity gene. Diabetes 45:1455-1462. https://doi.org/10.2337/diab.45.11.1455
  27. Yang S, Koteish A, Lin H, Huang J, Roskams T, Dawson V, Diehl AM. 2004. Oval cells compensate for damage and replicative senescence of mature hepatocytes in mice with fatty liver disease. Hepatology 39: 403-411. https://doi.org/10.1002/hep.20082
  28. Norman D, Isidori AM, Frajese V, Caprio M, Chew SL, Grossman AB, Clark AJ, Michael Besser G, Fabbri A. 2003. ACTH and ${\alpha}$-MSH inhibit leptin expression and secretion in 3T3-L1 adipocytes: model for a central-peripheral melanocortin-leptin pathway. Mol Cell Endocrinol 28: 99-109.
  29. Kwon JY, Ann IS, Park KY, Cheigh HS, Song YO. 2005. The beneficial effects of pectin on obesity in vitro and in vivo. J Korean Soc Food Sci Nutr 34: 13-20. https://doi.org/10.3746/jkfn.2005.34.1.013
  30. Matsuzawa Y, Funahashi T, Kihara S, Shimomura I. 2004. Adiponectin and metabolic syndrome. Arterioscler Thromb Vasc Biol 24: 29-33. https://doi.org/10.1161/01.ATV.0000099786.99623.EF
  31. Pineiro R, Iglesias MJ, Gallego R, Raghay K, Eiras S, Rubio J, Dieguez C, Gualillo O, González-Juanatey JR, Lago F. 2005. Adiponectin is synthesized and secreted by human and murine cardiomyocytes. FEBS Lett 579: 5163-5169. https://doi.org/10.1016/j.febslet.2005.07.098
  32. Ashima RS, Flier JS. 2000. Adipose tissue as an endocrine organ. Trends Endocrinol Metab 11: 327-332. https://doi.org/10.1016/S1043-2760(00)00301-5
  33. Hotamisligil GS, Shargill NS, Spiegelman BM. 1993. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science 259: 87-91. https://doi.org/10.1126/science.7678183
  34. del Aguila LF, Claffey KP, Kirwan JP. 1999. TNF-alpha impairs insulin signaling and insulin stimulation of glucose uptake in C2C12 muscle cells. Am J Physiol 276: E849-E855.
  35. Engelman JA, Berg AH, Lewis RY, Lisanti MP, Scherer PE. 2000. Tumor necrosis factor alpha-mediated insulin resistance, but not dedifferentiation, is abrogated by MEK1/2 inhibitors in 3T3-L1 adipocytes. Mol Endocrinol 14: 1557-1569.
  36. Levy JR, Davenport B, Clore JN, Stevens W. 2002. Lipid metabolism and resistin gene expression in insulin-resistant Fischer 344 rats. Am J Physiol Endocrinol Metab 282:E626-E633. https://doi.org/10.1152/ajpendo.00346.2001
  37. Zhang J, Qin Y, Zheng X, Qiu J, Gong L, Mao H, Jia W, Guo J. 2002. The relationship between human serum resistin level and body fat content, plasma glucose as well as blood pressure. Zhonghua Yi Xue Za Zhi 82: 1609-1612.
  38. Kim KH, Lee K, Moon YS, Sul HS. 2001. A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation. J Biol Chem 276: 11252-11256. https://doi.org/10.1074/jbc.C100028200
  39. Shojima N, Sakoda H, Ogihara T, Fujishiro M, Katagiri H, Anai M, Onishi Y, Ono H, Inukai K, Abe M, Fukushima Y, Kikuchi M, Oka Y, Asano T. 2002. Humoral regulation of resistin expression in 3T3-L1 and mouse adipose cells. Diabetes 51: 1737-1744. https://doi.org/10.2337/diabetes.51.6.1737
  40. Pakala R, Kuchulakanti P, Rha SW, Cheneau E, Baffour R, Waksman R. 2004. Peroxisome proliferator-activated receptor gamma: its role in metabolic syndrome. Cardiovasc Radiat Med 5(2): 97-103. https://doi.org/10.1016/j.carrad.2004.03.006
  41. Sharma AK, Bharti S, Ojha S, Bhatia J, Kumar N, Ray R, Kumari S, Arya DS. 2011. Up-regulation of PPAR-${\gamma}$, heat shock protein-27 and -72 by naringin attenuates insulin resistance, ${\beta}$-cell dysfunction, hepatic steatosis and kidney damage in a rat model of type 2 diabetes. Br J Nutr 106:1713-1723. https://doi.org/10.1017/S000711451100225X
  42. Maeda N, Takahashi M, Funahashi T, Kihara S, Nishizawa H, Kishida K, Nagaretani H, Matsuda M, Komuro R, Ouchi N, Kuriyama H, Hotta K, Nakamura T, Shimomura I, Matsuzawa Y. 2001. PPAR-${\gamma}$ ligands increase expression and plasma concentrations of adiponectin, an adipose- derived protein. Diabetes 50: 2094-2099. https://doi.org/10.2337/diabetes.50.9.2094
  43. Harmon AW, Harp JB. 2001. Differential effects of flavonoids on 3T3-L1 adipogenesis and lipolysis. Am J Physiol Cell Physiol 280: C807-C813.
  44. Kang S, Kim D, Lee BH, Kim MR, Chiang M, Hong J. 2011. Antioxidant properties and cytotoxic effects of fractions from glasswort (Salicornia herbacea L.) seed extracts on human intestinal cells. Food Sci Biotechnol 20: 115-122. https://doi.org/10.1007/s10068-011-0016-7

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