The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Characteristics of a Lettuce mosaic virus Isolate Infecting Lettuce in Korea

  • Lim, Seungmo (Department of Biosystems and Bioengineering, University of Science and Technology) ;
  • Zhao, Fumei (Department of Biosystems and Bioengineering, University of Science and Technology) ;
  • Yoo, Ran Hee (Department of Biosystems and Bioengineering, University of Science and Technology) ;
  • Igori, Davaajargal (Department of Biosystems and Bioengineering, University of Science and Technology) ;
  • Lee, Su-Heon (Institute of Plant Medicine, Kyungpook National University) ;
  • Lim, Hyoun-Sub (Department of Applied Biology, Chungnam National University) ;
  • Moon, Jae Sun (Department of Biosystems and Bioengineering, University of Science and Technology)
  • Received : 2013.12.26
  • Accepted : 2014.01.14
  • Published : 2014.06.01
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Abstract

Lettuce mosaic virus (LMV) causes disease of plants in the family Asteraceae, especially lettuce crops. LMV isolates have previously been clustered in three main groups, LMV-Yar, LMV-Greek and LMV-RoW. The first two groups, LMV-Yar and LMV-Greek, have similar characteristics such as no seed-borne transmission and non-resistance-breaking. The latter one, LMV-RoW, comprising a large percentage of the LMV isolates contains two large subgroups, LMV-Common and LMV-Most. To date, however, no Korean LMV isolate has been classified and characterized. In this study, LMV-Muju, the Korean LMV isolate, was isolated from lettuce showing pale green and mottle symptoms, and its complete genome sequence was determined. Classification method of LMV isolates based on nucleotide sequence divergence of the NIb-CP junction showed that LMV-Muju was categorized as LMV-Common. LMV-Muju was more similar to LMV-O (LMV-Common subgroup) than to LMV-E (LMV-RoW group but not LMV-Common subgroup) even in the amino acid domains of HC-Pro associated with pathogenicity, and in the CI and VPg regions related to ability to overcome resistance. Taken together, LMV-Muju belongs to the LMV-Common subgroup, and is expected to be a seed-borne, non-resistance-breaking isolate. According to our analysis, all other LMV isolates not previously assigned to a subgroup were also included in the LMV-RoW group.

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References

  1. Abdul-Razzak, A., Guiraud, T., Peypelut, M., Walter, J., Houvenaghel, M. C., Candresse, T., Gall, O. L. and German-Retana, S. 2009. Involvement of the cylindrical inclusion (CI) protein in the overcoming of an eIF4E-mediated resistance against Lettuce mosaic potyvirus. Mol. Plant Pathol. 10: 109-113. https://doi.org/10.1111/j.1364-3703.2008.00513.x
  2. Atreya, C. D., Atreya, P. L., Thornbury, D. W. and Pirone, T. P. 1992. Site-directed mutations in the potyvirus HC-Pro gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants. Virology 191:106-111. https://doi.org/10.1016/0042-6822(92)90171-K
  3. Atreya, C. D. and Pirone, T. P. 1993. Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility. Proc. Natl. Acad. Sci. USA. 90:11919-11923. https://doi.org/10.1073/pnas.90.24.11919
  4. Ballut, L., Drucker, M., Pugniere, M., Cambon, F., Blanc, S., Roquet, F., Candresse, T., Schmid, H. P., Nicolas, P., Gall, O. L. and Badaoui, S. 2005. HcPro, a multifunctional protein encoded by a plant RNA virus, targets the 20S proteasome and affects its enzymic activities. J. Gen. Virol. 86:2595-2603. https://doi.org/10.1099/vir.0.81107-0
  5. Bos, L., Huijberts, N. and Cuperus, C. 1994. Further observations on variation of lettuce mosaic virus in relation to lettuce (Lactuca sativa), and a discussion of resistance terminology. Eur. J. Plant Pathol. 100:293-314. https://doi.org/10.1007/BF01876442
  6. Dolja, V. V., McBride, H. J. and Carrington, J. C. 1992. Tagging of plant potyvirus replication and movement by insertion of beta-glucuronidase into the viral polyprotein. Proc. Natl. Acad. Sci. USA. 89:10208-10212. https://doi.org/10.1073/pnas.89.21.10208
  7. German-Retana, S., Walter, J. and Gall, O. L. 2008. Lettuce mosaic virus: from pathogen diversity to host interactors. Mol. Plant Pathol. 9:127-136. https://doi.org/10.1111/j.1364-3703.2007.00451.x
  8. Klein, P. G., Klein, R. R., Rodriguez-Cerezo, E., Hunt, A. G. and Shaw, J. G. 1994. Mutational analysis of the tobacco vein mottling virus genome. Virology 204:759-769. https://doi.org/10.1006/viro.1994.1591
  9. Krause-Sakate, R., Gall, O. L., Fakhfakh, H., Peypelut, M., Marrakchi, M., Varveri, C., Pavan, M. A., Souche, S., Lot, H., Zerbini, F. M. and Candresse, T. 2002. Molecular and Biological characterization of Lettuce mosaic virus (LMV) isolates reveals a distinct and widespread type of resistancebreaking isolate: LMV-Most. Phytopathology 92:563-572. https://doi.org/10.1094/PHYTO.2002.92.5.563
  10. Krause-Sakate, R., Fakhfakh, H., Peypelut, M., Pavan, M. A., Zerbini, F. M., Marrakchi, M., Candresse, T. and Gall, O. L. 2004. A naturally occurring recombinant isolate of Lettuce mosaic virus. Arch. Virol. 149:191-197.
  11. Nebreda, M., Moreno, A., Perez, N., Palacios, I., Seco-Fernandez, V. and Fereres, A. 2004. Activity of aphids associated with lettuce and broccoli in Spain and their efficiency as vectors of Lettuce mosaic virus. Virus Res. 100:83-88. https://doi.org/10.1016/j.virusres.2003.12.016
  12. Peypelut, M., Krause-Sakate, R., Guiraud, T., Pavan, M. A., Candresse, T., Zerbini, F. M. and Gall, O. L. 2004. Specific detection of lettuce mosaic virus isolates belonging to the "Most" type. J. Virol. Methods 121:119-124. https://doi.org/10.1016/j.jviromet.2004.06.005
  13. Pink, D. A. C., Lot, H. and Johnson, R. 1992. Novel pathotypes of lettuce mosaic virus - breakdown of a durable resistance? Euphytica 63:169-174. https://doi.org/10.1007/BF00023921
  14. Redondo, E., Krause-Sakate, R., Yang, S. J., Lot, H., Gall, O. L. and Candresse, T. 2001. Lettuce mosaic virus pathogenicity determinants in susceptible and tolerant lettuce cultivars map to different regions of the viral genome. Mol. Plant-Microbe Interact. 14:804-810. https://doi.org/10.1094/MPMI.2001.14.6.804
  15. Revers, F., Yang, S. J., Walter, J., Souche, S., Lot, H., Gall, O. L., Candresse, T. and Dunez, J. 1997a. Comparison of the complete nucleotide sequences of two isolates of lettuce mosaic virus differing in their biological properties. Virus Res. 47:167-177. https://doi.org/10.1016/S0168-1702(96)01411-6
  16. Revers, F., Lot, H., Souche, S., Gall, O. L., Candresse, T. and Dunez, J. 1997b. Biological and molecular variability of lettuce mosaic virus isolates. Phytopathology 87:397-403. https://doi.org/10.1094/PHYTO.1997.87.4.397
  17. Tavert-Roudet, G., Abdul-Razzak, A., Doublet, B., Walter, J., Delaunay, T., German-Retana, S., Michon, T., Gall, O. L. and Candresse, T. 2012. The C terminus of lettuce mosaic potyvirus cylindrical inclusion helicase interacts with the viral VPg and with lettuce translation eukaryotic initiation factor 4E. J. Gen. Virol. 93:184-193. https://doi.org/10.1099/vir.0.035881-0

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