Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
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Neuroprotective Effects of Hyangsayangwi-tang in MPTP-induced Mouse Model of Parkinson's Disease

MPTP(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine)로 유도된 Parkinson's Disease 동물 모델을 이용한 향사양위탕의 신경 세포 보호 효과

  • Go, Ga-Yeon (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Daejeon university) ;
  • Kim, Yun-Hee (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Daejeon university) ;
  • Ahn, Taek-Won (Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Daejeon university)
  • 고가연 (대전대학교 한의과대학 사상체질의학) ;
  • 김윤희 (대전대학교 한의과대학 사상체질의학) ;
  • 안택원 (대전대학교 한의과대학 사상체질의학)
  • Received : 2014.06.12
  • Accepted : 2014.06.29
  • Published : 2014.06.30
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Abstract

Objectives To evaluate the neuroprotective effects of Hyangsayangwi-tang (HY), a Korean traditional medicinal prescription in a Parkinson's disease mouse model. Methods Four groups(each of 10 mouse per group) were used in this study. The neuroprotective effect of HY was examined in a Parkinson's disease mouse model. C57BL/6 mouse treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30mg/kg/day), intraperitoneal (i.p.) for 5 days. Slow behavioral responses and memory disorder is the major clinical symptoms of PD. In order to investigate the effect of HY on recovery of behavioral deficits and memory, we examined the motor function and memory by using Morris water maze and Forced swimming test. Ischemic mouse brain stained with TTC(2,3,5 triphenyl tetrazolium chloride) in the MPTP-induced Parkinson's disease to find out ischemia and tissue damage in mouse. The convenient, simple, and accurate high-performance liquid chromatography (HPLC) method was established for simultaneous determination of neurotransmitters in MPTP-HY group. To measure the amount of dopamine in mice brain, striatum-substantia nigra, was examined by Bradford assay. Immunohistochemistry was examined in the MPTP-induced Parkinson's disease (PD) mouse to evaluate the neuroprotective effects of Hyangsayangwi-tang on hippocampal lesion, ST and SNpc. Results and Conclusions Hyangsayangwi-tang (HY) prevents MPTP-induced loss of serotonin, hippocampus and TH-ir cell.

Keywords

References

  1. Imaki T, Katsumata H, Miyata M, Naruse M, Imaki J, Minami S. Expression of corticotropin-releasing hormone type 1 receptor in paraventricular nucleus after acute stress. Neuroendocrinology. 2001;73(5):293-301. https://doi.org/10.1159/000054646
  2. Bae NY, Yang HO, Ahn TW. Protection effect of New-Yeolda-Hanso tang $against\beta$-Amyloid Induced Cytotoxicity in NGF-differentiated PC12 Cells. J Sasang Constitut Med. 2009;21:138-153. (Korean)
  3. Bae NY, Ahn TK, Chung SK, Oh MS, Ko HS, Oh HG et al. The neuroprotective effect of modified Yeoldahanso-tang via autophagy enhancement in modelsof Parkinson's disease. Journal of Ethnopharmacology. 2011;134:313-322. (Korean) https://doi.org/10.1016/j.jep.2010.12.016
  4. Dauer W, Przedborski S. Parkinson's disease: mechanisms and models. Neuron. 2003;39:889-909. https://doi.org/10.1016/S0896-6273(03)00568-3
  5. Seo JH, Shin MS, Kim CJ, Lee KS, lim BB. The influence of treadmill exercise in hot environments about serotonin expression in the rat brain, generating neurons and short-term memory. Korean Journal of Sport Science. 2009;20(4);744. (Korean)
  6. Smith GW, Aubry JM, Dellu F, Contarino A, Bilezikjian LM, Gold LH, et al. Corticotropin releasing factor receptor 1-deficient micedisplay decreased anxiety, impaired stress response, and aberrant neuroendocrine development. Neuron. 1998;20(6):1093-102. https://doi.org/10.1016/S0896-6273(00)80491-2
  7. Porsolt RD, Anton G, Blavet N et al. Behavioral despair in rats : A new model sensitive to antidepressant treatments. Eur J Pharmacol 1978;47:379-391. https://doi.org/10.1016/0014-2999(78)90118-8
  8. Porsolt RD. Animal model of depression. Biomedicine 1979;30:139-140.
  9. Langston, J.W., Irwin, I., Langston, E.B., Forno, L.S. Pargyline prevents MPTP-induced parkinsonism in primates. Science 225(4669):1460-1462, 1984. https://doi.org/10.1126/science.6433484
  10. Arai N, Isaji M, Kojima M, Mizuta E, Kuno S. Combined effects of cabergoline and L-dopa on parkinsonism in MPTP-treated cynomolgus monkeys. J Neural Transmission 103(11):1037-1316, 1996.
  11. Lau, Y.S., Meredith, G.E. From drugs of abuse to parkinsonism: The MPTP Mouse Model of Parkinson's Disease. Methods in Molecular Medicine 79(1): 103-116, 2003.
  12. Lee JM. Dongeuisoosebowonsasangbonchokwon. Seoul: Eulyumoonhwasa. 2002:27-278. (Korean)
  13. The National College of Oriental Medicine Sasangeuihakclassroom. Sasangeuihak. Seoul: Jipmundang. 2004: 260,265,276,394-5,442-80. (Korean)
  14. Song IB et all. Sasangeuihak. Seoul:Jipmundang. 2004:343,366. (Korean)
  15. Lee JC. Sinsasangeuihakron II. Seoul:Mokkwato. 2001:79-83. (Korean)
  16. Jeong BJ, Kim JW, Kim BC, Woo SH, Na YJ, Shin HJ et al. A case of tremor in Parkinson's disease treated with Korean medicine. Korean J. Orient.int.Med. 2006:27(4)954-961. (Korean)
  17. Bae NY. Neuroprotective effect of modified Yeoldahanso-tang via autophagy enhancement on Parkinsonian model. Dae-jeon National University. 2013;25(3):208-217. (Korean)
  18. Brian R. Perri, Douglas H. Smith, Hisayuki Murai, Grant Sinson, Kathryn E. Saatman, Ramesh Raghupathl, Raymond T. Bartus, Tracy K. Mcintosh. Metabolic Quantification of Lesion Volume following Experimental Traumatic Brain Injury in the Rat. Journal of Neurotrauma. 1997;14:15-22. https://doi.org/10.1089/neu.1997.14.15
  19. Vosselera M., N. Abegunewardenea, N. Hoffmannb, S.E. Petersene, D. Beckera, D. Cleppienb, P. Kunzc, K.-F. Kreitnerc, M. Lauterbacha, B. Bierbachd, C. Duberc, T. Goria, T. Munzela, L.M. Schreiberb, G. Horsticka. Area at Risk and Viability after Myocardial Ischemia and Reperfusion Can Be Determined by Contrast-Enhanced Cardiac Magnetic Resonance Imaging. Eur Surg Res. 2009;43:13-23. https://doi.org/10.1159/000211716
  20. Kasper, Braunwald, Fauci, Hauser, Longo, Jameson. Harrison's Principles of Internal medicine. 16th edition. Seoul: MIP. 2006:2631-2638.
  21. Widdowson, P.S., Farnworth, M.J., Upton, R., Simpson, M.G. No changes in behavior, nigro-striatal system neurochemistry or neuronal cell death following toxic multiple oral paraquat administration to rat. Human and Experomental Toxicology. 1996; 15(7):583-591. https://doi.org/10.1177/096032719601500706
  22. Ferger, B., Teismann, P., Earl, C,D., Kuschinsky, K., Oertel, W.H. The propective effects of PBN against MPTP toxocity are independent of hydroxyl radical trapping. Phamaco Biochem Behav. 2000;65(3): 425-431. https://doi.org/10.1016/S0091-3057(99)00229-4
  23. Westm B.D., Sheghrue, P.J., Vanko, A.E.H,, Ransorn, R.W. Kinney, G.G. Amphetamine-induced locomotor activity is reduced in mice following MPTP treatment but not following selegiline/MPTP treatment. Pharmacol. Biochm. Behav 2006;84(1):158-161. https://doi.org/10.1016/j.pbb.2006.04.022
  24. Sun, L., Xu, S., Zhou, M., Wang, C., Wu, Y., Chan, P. Effects of cysteamine on MPTP-induced dopaminergic neurodegeneration in mice. Brain Research. 2010;1335:74-82. https://doi.org/10.1016/j.brainres.2010.03.079
  25. Cummings JL. Depression in Parkinson's disease : a review. Am J Psychiatry 1992;149:443-454. https://doi.org/10.1176/ajp.149.4.443
  26. Fetoni V, Soliveri P, Monza D, Testa D, Girotti F. Affective symptoms in multiple system atropy and Parkinson's disease : response to levodopa therapy. J Neurol Neurosurg Psychiatry. 1999;66:541-544. https://doi.org/10.1136/jnnp.66.4.541
  27. Burn DJ. Depression in Parkinson's disease. Eur J Neurol 9. 2002:44-54. https://doi.org/10.1046/j.1468-1331.9.s3.6.x

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  2. Neuroprotective Effects of Modified Yuldahanso-tang (MYH) in a Parkinson's Disease Mouse Model vol.27, pp.2, 2015, https://doi.org/10.7730/JSCM.2015.27.2.270