Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Platycosides from the Roots of Platycodon grandiflorum and Their Health Benefits

  • Nyakudya, Elijah (Research Center for Industrial Development of Biofood Materials, Chonbuk National University) ;
  • Jeong, Jong Hoon (Research Center for Industrial Development of Biofood Materials, Chonbuk National University) ;
  • Lee, Nam Keun (Research Center for Industrial Development of Biofood Materials, Chonbuk National University) ;
  • Jeong, Yong-Seob (Department of Food Science and Technology, Chonbuk National University)
  • Received : 2014.02.21
  • Accepted : 2014.04.10
  • Published : 2014.06.30
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Abstract

The extracts and pure saponins from the roots of Platycodon grandiflorum (PG) are reported to have a wide range of health benefits. Platycosides (saponins) from the roots of PG are characterized by a structure containing a triterpenoid aglycone and two sugar chains. Saponins are of commercial significance, and their applications are increasing with increasing evidence of their health benefits. The biological effects of saponins include cytotoxic effects against cancer cells, neuroprotective activity, antiviral activity, and cholesterol lowering effects. Saponins with commercial value range from crude plant extracts, which can be used for their foaming properties, to high purity saponins such as platycodin D, which can be used for its health applications (e.g., as a vaccine adjuvant). This review reveals that platycosides have many health benefits and have the potential to be used as a remedy against many of the major health hazards (e.g., cancer, obesity, alzheimer's) faced by populations around the world. Methods of platycoside purification and analysis are also covered in this review.

Keywords

References

  1. Han LK, Xu BJ, Kimura Y, Zheng YN, Okuda H. 2000. Platycodi radix affects lipid metabolism in mice with high fat diet-induced obesity. J Nutr 130: 2760-2764. https://doi.org/10.1093/jn/130.11.2760
  2. Han LK, Zheng YN, Xu BJ, Okuda H, Kimura Y. 2002. Saponins from Platycodi radix ameliorate high fat diet induced obesity in mice. J Nutr 132: 2241-2245. https://doi.org/10.1093/jn/132.8.2241
  3. Ashok BT, Ali R. 1999. The aging paradox: free radical theory of aging. Exp Gerontol 34: 293-303. https://doi.org/10.1016/S0531-5565(99)00005-4
  4. Finkel T, Holbrook NJ. 2000. Oxidants, oxidative stress and the biology of ageing. Nature 408: 239-247. https://doi.org/10.1038/35041687
  5. Halliwell B. 2006. Oxidative stress and neurodegeneration: where are we now? J Neurochem 97: 1634-1658. https://doi.org/10.1111/j.1471-4159.2006.03907.x
  6. Halliwell B. 2007. Oxidative stress and cancer: have we moved forward? Biochem J 401: 1-11. https://doi.org/10.1042/BJ20061131
  7. Tiwari AK. 2001. Imbalance in antioxidant defense and human diseases: multiple approach of natural antioxidant therapy. Curr Sci 81: 1179-1187.
  8. Zhao HL, Harding SV, Marinangeli CP, Kim YS, Jones PJ. 2008. Hypocholesterolemic and anti-obesity effects of saponins from Platycodon grandiflorum in hamsters fed atherogenic diets. J Food Sci 73: H195-H200. https://doi.org/10.1111/j.1750-3841.2008.00915.x
  9. Evans P, Halliwell B. 2001. Micronutrients: oxidant/antioxidant status. Br J Nutr 85: S67-S74. https://doi.org/10.1079/BJN2000296
  10. Choi YH, Yoo DS, Cha MR, Choi CW, Kim YS, Choi SU, Lee KR, Ryu SY. 2010. Antiproliferative effects of saponins from the roots of Platycodon grandiflorum on cultured human tumor cells. J Nat Prod 73: 1863-1867. https://doi.org/10.1021/np100496p
  11. Takagi K, Lee EB. 1972. Pharmacological studies on Platycodon grandiflorum A. DC. 3. Activities of crude platycodin on respiratory and circulatory systems and its other pharmacological activities. Yakugaku Zasshi 92: 969-973. https://doi.org/10.1248/yakushi1947.92.8_969
  12. Lee EB. 1973. Pharmacological studies on Platycodon grandiflorum A. DC. IV. A comparison of experimental pharmacological effects of crude platycodin with clinical indications of Platycodi Radix. Yakugaku Zasshi 93: 1188-1194. https://doi.org/10.1248/yakushi1947.93.9_1188
  13. San Martin R, Briones R. 1999. Industrial uses and sustainable supply of Quillaja saponaria (Rosaceae) saponins. Econ Bot 53: 302-311. https://doi.org/10.1007/BF02866642
  14. Guclu-Ustundag O, Mazza G. 2007. Saponins: properties, applications and processing. Crit Rev Food Sci Nutr 47: 231-258. https://doi.org/10.1080/10408390600698197
  15. Ha YW, Na YC, Seo JJ, Kim SN, Linhardt RJ, Kim YS. 2006. Qualitative and quantitative determination of ten major saponins in Platycodi Radix by high performance liquid chromatography with evaporative light scattering detection and mass spectrometry. J Chromatogr A 1135: 27-35. https://doi.org/10.1016/j.chroma.2006.09.015
  16. Ryu CS, Kim CH, Lee SY, Lee KS, Choung KJ, Song GY, Kim BH, Ryu SY, Lee HS, Kim SK. 2012. Evaluation of the total oxidant scavenging capacity of saponins isolated from Platycodon grandiflorum. Food Chem 132: 333-337. https://doi.org/10.1016/j.foodchem.2011.10.086
  17. Jeong CH, Choi GN, Kim JH, Kwak JH, Kim DO, Kim YJ, Hoe HJ. 2010. Antioxidant activities of the aerial parts of Platycodon grandiflorum. Food Chem 118: 278-282. https://doi.org/10.1016/j.foodchem.2009.04.134
  18. Kim JW, Lee SW, Park SJ, Shin JC, Yang JW, Lim JH. 2011. Pharmaceutical composition for preventing or treating hepatitis C, comprising the root extracts of Platycodon grandiflorum or Platycodon grandiflorum saponin components. US Patent Application 2011/0274656 A1.
  19. Francis G, Kerem Z, Makkar HP, Becker K. 2002. The biological action of saponins in animal systems: a review. Br J Nutr 88: 587-605. https://doi.org/10.1079/BJN2002725
  20. Rao AV, Gurfinkel DM. 2000. The bioactivity of saponins: triterpenoid and steroidal glycosides. Drug Metabol Drug Interact 17: 211-235.
  21. Sun HX, Xie Y, Ye YP. 2009. Advances in saponin-based adjuvants. Vaccine 27: 1787-1796. https://doi.org/10.1016/j.vaccine.2009.01.091
  22. Kensil CR. 1996. Saponins as vaccine adjuvants. Crit Rev Ther Drug Carrier Syst 13: 1-55.
  23. Na YC, Ha YW, Kim YS, Kim KJ. 2008. Structural analysis of platycosides in Platycodi Radix by liquid chromatography/ electrospray ionization-tandem mass spectrometry. J Chromatogr A 1189: 467-475. https://doi.org/10.1016/j.chroma.2007.11.085
  24. Ha IJ, Ha YW, Kang MS, Lee JS, Park DH, Kim YS. 2010. Enzymatic transformation of platycosides and one-step separation of platycodin D by high-speed countercurrent chromatography. J Sep Sci 33: 1916-1922. https://doi.org/10.1002/jssc.200900842
  25. Li W, Zhang W, Xiang L, Wang Z, Zheng YN, Wang YP, Zhang J, Chen L. 2010. Platycoside N: a new oleanane-type triterpenoid saponin from the roots of Platycodon grandiflorum. Molecules 15: 8702-8708. https://doi.org/10.3390/molecules15128702
  26. Xie Y, Sun HX, Li D. 2009. Platycodin D is a potent adjuvant of specific cellular and humoral immune responses against recombinant hepatitis B antigen. Vaccine 27: 757-764. https://doi.org/10.1016/j.vaccine.2008.11.029
  27. Bensky D, Gamble A. 1993. Chinese Herbal Medicine: Materia Medica. 3rd ed. Eastland Press, Seattle, WA, USA. p 429-432.
  28. Saeki T, Koike K, Nikaido T. 1999. A comparative study on commercial, botanical gardens and wild samples of the roots of Platycodon grandiflorum by HPLC analysis. Planta Med 65: 428-431. https://doi.org/10.1055/s-1999-14021
  29. Yan YZ, Xue JC, Wu JR, Yoo DS, Lee SY, Kim YK, Uddin MR, Park SU. 2012. Variation of triterpenoid saponin content in Platycodon grandiflorum (Jacq.) A.D.C. Asian J Chem 24: 1268-1270.
  30. Ishii H, Tori K, Tozyo T, Yoshimura Y. 1984. Saponins from roots of Platycodon grandiflorum. Part 2. Isolation and structure of new triterpene glycosides. J Chem Soc Perkin Trans 1661-668.
  31. Choi CY, Kim JY, Kim YS, Chung YC, Hahm KS, Jeong HG. 2001. Augmentation of macrophage functions by an aqueous extract isolated from Platycodon grandiflorum. Cancer Lett 166: 17-25. https://doi.org/10.1016/S0304-3835(01)00440-2
  32. Choi CY, Kim JY, Kim YS, Chung YC, Seo JK, Jeong HG. 2001. Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor necrosis factoralpha from murine macrophages. Int Immunopharmacol 1: 1141-1151. https://doi.org/10.1016/S1567-5769(01)00047-9
  33. Kim YP, Lee EB, Kim SY, Li DW, Ban HS, Lim SS, Shin KH, Ohuchi K. 2001. Inhibition of prostaglandin E2 production by platycodin D isolated from the root of Platycodon grandiflorum. Planta Med 67: 362-364. https://doi.org/10.1055/s-2001-14317
  34. Jeong EK, Cha HJ, Ha YW, Kim YS, Ha IJ, Na YC. 2010. Development and optimization of a method for the separation of platycosides in Platycodi Radix by comprehensive two-dimensional liquid chromatography with mass spectrometric detection. J Chromatogr A 1217: 4375-4382. https://doi.org/10.1016/j.chroma.2010.04.053
  35. Ahn KS, Hahn BS, Kwack K, Lee EB, Kim YS. 2006. Platycodin D-induced apoptosis through nuclear factor-$\kappa$B activation in immortalized keratinocytes. Eur J Pharmacol 537: 1-11. https://doi.org/10.1016/j.ejphar.2006.03.012
  36. Zhao HL, Cho KH, Ha YW, Jeong TS, Lee WS, Kim YS. 2006. Cholesterol-lowering effect of platycodin D in hypercholesterolemic ICR mice. Eur J Pharmacol 537: 166-173. https://doi.org/10.1016/j.ejphar.2006.03.032
  37. Zhao HL, Kim YS. 2004. Determination of the kinetic properties of platycodin D for the inhibition of pancreatic lipase using a 1,2-diglyceride-based colorimetric assay. Arch Pharm Res 27: 968-972. https://doi.org/10.1007/BF02975852
  38. Zhang L, Liu ZJ, Tian JK. 2007. Cytotoxic triterpenoid saponins from the roots of Platycodon grandiflorum. Molecules 12: 832-841. https://doi.org/10.3390/12040832
  39. Lee JY, Hwang WI, Lim ST. 2004. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J Ethnopharmacol 93: 409-415. https://doi.org/10.1016/j.jep.2004.04.017
  40. Tada A, Kaneiwa Y, Shoji J, Shibata S. 1975. Studies on the saponins of the root of Platycodon grandiflorum A. De Candolle. I. Isolation and the structure of platycodin-D. Chem Pharm Bull (Tokyo) 23: 2965-2972. https://doi.org/10.1248/cpb.23.2965
  41. Park YS, Yoon YS, Ahn HS. 2007. Platycodon grandiflorum extract represses up-regulated adipocyte fatty acid binding protein triggered by a high fat feeding in obese rats. World J Gastroenterol 13: 3493-3499. https://doi.org/10.3748/wjg.v13.i25.3493
  42. Wu J, Yang G, Wen W, Zhang F, Yuan J, An L. 2011. Cholesterol metabolism regulation and antioxidant effect of platycodin D on hyperlipidemic emulsion-reduced rats. Afr J Pharm Pharmacol 5: 2444-2453.
  43. Zhao HL, Sim JS, Shim SH, Ha YW, Kang SS, Kim YS. 2005. Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction. Int J Obes (Lond) 29: 983-990. https://doi.org/10.1038/sj.ijo.0802948
  44. Kay AB. 2000. Overview of 'allergy and allergic diseases: with a view to the future'. Br Med Bull 56: 843-864. https://doi.org/10.1258/0007142001903481
  45. Oh YC, Kang OH, Choi JG, Lee YS, Brice OO, Jung HJ, Hong SH, Lee YM, Shin DW, Kim YS, Kwon DY. 2010. Antiallergic activity of a platycodon root ethanol extract. Int J Mol Sci 11: 2746-2758. https://doi.org/10.3390/ijms11072746
  46. Lee SJ, Bang WS, Hong JS, Kwon OJ, Shin SR, Yoon KY. 2013. Antioxidant and antimicrobial activities of black Doraji (Platycodon grandiflorum). Korean J Food Preserv 20: 510-517. https://doi.org/10.11002/kjfp.2013.20.4.510
  47. Zhang L, Ravipati AS, Koyyalamudi SR, Jeong SC, Reddy N, Bartlett J, Smith PT, de la Cruz M, Monteiro MC, Melguizo A, Jiménez E, Vicente F. 2013 Anti-fungal and anti-bacterial activities of ethanol extracts of selected traditional Chinese medicinal herbs. Asian Pac J Trop Med 6: 673-681. https://doi.org/10.1016/S1995-7645(13)60117-0
  48. Cacabelos R. 1997. Alzheimer disease. Rev Med Pract Clin 2: 124-142.
  49. Son IH, Park YH, Lee SI, Yang HD, Moon HI. 2007. Neuroprotective activity of triterpenoid saponins from Platycodi Radix against glutamate-induced toxicity in primary cultured rat cortical cells. Molecules 12: 1147-1152. https://doi.org/10.3390/12051147
  50. Ha YW, Kim YS. 2009. Preparative isolation of six major saponins from Platycodi Radix by high-speed counter-current chromatography. Phytochem Anal 20: 207-213. https://doi.org/10.1002/pca.1116
  51. Li W, Zhao LC, Wang Z, Zheng YN, Liang J, Wang H. 2012. Response surface methodology to optimize enzymatic preparation of deapio-platycodin d and platycodin d from radix platycodi. Int J Mol Sci 13: 4089-4100. https://doi.org/10.3390/ijms13044089
  52. Wie HJ, Zhao HL, Chang JH, Kim YS, Hwang IK, Ji GE. 2007. Enzymatic modification of saponins from Platycodon grandiflorum with Aspergillus niger. J Agric Food Chem 55: 8908-8913. https://doi.org/10.1021/jf0716937
  53. Park JS, Rho HS, Kim DH, Chang IS. 2006. Enzymatic preparation of kaempferol from green tea seed and its antioxidant activity. J Agric Food Chem 54: 2951-2956. https://doi.org/10.1021/jf052900a
  54. Ko SR, Choi KJ, Suzuki K, Suzuki Y. 2003. Enzymatic preparation of ginsenosides Rg2, Rh1 and F1. Chem Pharm Bull (Tokyo) 51: 404-408. https://doi.org/10.1248/cpb.51.404
  55. Ko SR, Suzuki Y, Choi KJ, Kim YH. 2000. Enzymatic preparation of genuine prosapogenin, 20(S)-ginsenoside Rh1, from ginsenosides Re and Rg1. Biosci Biotechnol Biochem 64: 2739-2743. https://doi.org/10.1271/bbb.64.2739
  56. Oleszek W, Bialy Z. 2006. Chromatographic determination of plant saponins−an update (2002-2005). J Chromatogr A 1112: 78-91. https://doi.org/10.1016/j.chroma.2006.01.037
  57. Kitagawa I. 1986. Method of isolating soyasaponins. US Patent US4594412A.
  58. Oleszek WA. 2002. Chromatographic determination of plant saponins. J Chromatogr A 967: 147-162. https://doi.org/10.1016/S0021-9673(01)01556-4
  59. Kite GC, Porter EA, Simmonds MS. 2007. Chromatographic behavior of steroidal saponins studied by high-performance liquid chromatography-mass spectrometry. J Chromatogr A 1148: 177-183. https://doi.org/10.1016/j.chroma.2007.03.012
  60. Gurib-Fakim A. 2006. Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med 27: 1-93. https://doi.org/10.1016/j.mam.2005.07.008
  61. Bush TM, Rayburn KS, Holloway SW, Sanchez-Yamamoto DS, Allen BL, Lam T, So BK, Tran de H, Greyber ER, Kantor S, Roth LW. 2007. Adverse interactions between herbal and dietary substances and prescription medications: a clinical survey. Altern Ther Health Med 13: 30-35.
  62. Gagnier JJ, DeMelo J, Boon H, Rochon P, Bombardier C. 2006. Quality of reporting of randomized controlled trials of herbal medicine interventions. Am J Med 119: e1-e11. https://doi.org/10.1016/j.amjmed.2006.01.007
  63. Lee WH, Gam CO, Ku SK, Choi SH. 2011. Single oral dose toxicity test of platycodin D, a saponin from Platycodin Radix in mice. Toxicol Res 27: 217-224. https://doi.org/10.5487/TR.2011.27.4.217
  64. KFDA. 2009. Testing Guidelines for Safety Evaluation of Drugs. Notification No. 2009-116. Korea Food and Drug Administration. Sejong, Korea.

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  46. Conversion of Glycosylated Platycoside E to Deapiose-Xylosylated Platycodin D by Cytolase PCL5 vol.21, pp.4, 2020, https://doi.org/10.3390/ijms21041207
  47. The Pharmacological Effects and Health Benefits of Platycodon grandiflorus —A Medicine Food Homology Species vol.9, pp.2, 2020, https://doi.org/10.3390/foods9020142
  48. 도라지 종자 추출물의 처리가 제2형 당뇨 db/db 마우스의 혈당개선에 미치는 효과 vol.52, pp.1, 2020, https://doi.org/10.9721/kjfst.2020.52.1.81
  49. Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-ᴅ-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus vol.30, pp.6, 2014, https://doi.org/10.4014/jmb.2001.01041
  50. Immunomodulatory effects of fermented Platycodon grandiflorum extract through NF-κB signaling in RAW 264.7 cells vol.14, pp.5, 2020, https://doi.org/10.4162/nrp.2020.14.5.453
  51. The Effect of Different Water Extracts from Platycodon grandiflorum on Selected Factors Associated with Pathogenesis of Chronic Bronchitis in Rats vol.25, pp.21, 2014, https://doi.org/10.3390/molecules25215020
  52. Whole-genome, transcriptome, and methylome analyses provide insights into the evolution of platycoside biosynthesis in Platycodon grandiflorus , a medicinal plant vol.7, pp.None, 2014, https://doi.org/10.1038/s41438-020-0329-x
  53. Micelles self-assembled by 3- O -β- D -glucopyranosyl latycodigenin enhance cell membrane permeability, promote antibiotic pulmonary targeting and improve anti-infective efficacy vol.18, pp.1, 2014, https://doi.org/10.1186/s12951-020-00699-y
  54. 도라지(Platycodon grandiflorum)의 임상적 효과에 대한 체계적 문헌 고찰 vol.52, pp.1, 2014, https://doi.org/10.22889/kjp.2021.52.1.1
  55. Production of Bioactive Deapiosylated Platycosides from Glycosylated Platycosides in Balloon Flower Root Using the Crude Enzyme from the Food-Available Fungus Rhizopus oryzae vol.69, pp.16, 2014, https://doi.org/10.1021/acs.jafc.0c06756
  56. Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion vol.53, pp.5, 2014, https://doi.org/10.1038/s12276-021-00624-9
  57. Complete genome sequence of platycodon closterovirus 1, a novel putative member of the genus Closterovirus vol.166, pp.7, 2014, https://doi.org/10.1007/s00705-021-05081-3
  58. Uptake and Distribution Characteristic and Health Risk Assessment of Heavy Metal(loid)s in Platycodon Grandiflorum (Jacq.) A.DC. with Growth from a Medicinal Herb Garden of Xi’an, China vol.199, pp.7, 2014, https://doi.org/10.1007/s12011-020-02364-9
  59. Untargeted metabolomics approach reveals the tissue-specific markers of balloon flower root (Platycodi Radix) using UPLC-Q-TOF/MS vol.168, pp.None, 2014, https://doi.org/10.1016/j.microc.2021.106447
  60. Characterization of Saponins from Various Parts of Platycodon grandiflorum Using UPLC-QToF/MS vol.27, pp.1, 2022, https://doi.org/10.3390/molecules27010107