DOI QR코드

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Safety Profile Assessment and Identification of Volatile Compounds of Krill Eupausia superba Oil and Residues Using Different Extraction Methods

  • Haque, A.S.M. Tanbirul (Department of Food Science and Technology, Pukyong National University) ;
  • Kim, Seon-Bong (Department of Food Science and Technology, Pukyong National University) ;
  • Lee, Yang-Bong (Department of Food Science and Technology, Pukyong National University) ;
  • Chun, Byung-Soo (Department of Food Science and Technology, Pukyong National University)
  • 투고 : 2013.05.28
  • 심사 : 2013.12.30
  • 발행 : 2014.06.30

초록

In this study, Krill Eupausia superba oil was extracted using different solvents and supercritical carbon dioxide (SC-$CO_2$). During SC-$CO_2$ extraction, the pressure was set at 40 MPa and temperatures ranged from $40^{\circ}C$ to $55^{\circ}C$. We examined the differences in volatile compounds and safety profiles among extraction methods. Volatile compounds were determined using the thermal desorption system integrated with gas chromatography-mass spectrometry (GC-MS). Heavy metal content was analyzed by inductively coupled plasma mass spectrometry (ICP-MS). According to our results 10 volatile compounds were identified in krill sample. After SC-$CO_2$ extraction of oil, the concentrations of volatile compounds decreased, but increased after solvent extraction. In krill, heavy metal concentrations remained within the permissible limit. Moreover, Zn and Fe which have health benefits were detected at high concentrations. During a 90 days storage period at different temperatures, microbial activity was found to be lowest in SC-$CO_2$ extracted residues. Thus, the quality of krill oil and the residues obtained using SC-$CO_2$ extraction was higher and the oil was safer than those obtained using conventional solvent extraction. These results can be applied to the food industry to maintain high quality krill products.

키워드

참고문헌

  1. Alasalvar C, Al-Farsi M and Shahidi F. 2005. Compositional characteristics and antioxidant components of cherry laurel varieties and pekmez. Food Sci 70, S47-S52. https://doi.org/10.1111/j.1365-2621.2005.tb09064.x
  2. American Oil Chemists' Society (AOCS). 1998. Official Methods of Analysis. 5th ed. AOCS, Champaign, IL, US.
  3. Australia New Zealand Food Authority. 2002. Standards Code Part 1.4-Contaminants and Residues [Internet]. Food Standards Australia New Zealand (FSANZ), Accessed 7 Apr 2013, http://www.foodstandards.gov.au/code/Pages/default.aspx.
  4. Cappuccino JG and Sherman N. 2002. Microbiology: A Laboratory Manual. 6th ed. Dorling Kindersley Pvt. Ltd., Noida, IN, pp. 493-494.
  5. Chung HY, Yung IKS, Ma WCJ and Kim JS. 2002. Analysis of volatile components in frozen and dried scallops (Platinopecten yessoensis) by gas chromatography/mass spectrometry. Food Res Int 35,43-53. https://doi.org/10.1016/S0963-9969(01)00107-7
  6. Commission of the European Communities. 2002. Commission regulation (EC) No. 221/2002 of 6 February 2002 amending regulation (EC) No. 466/2002 setting maximum levels for certain contaminants in food stuffs. Off J Eur Commun Brussels, 6 February 2002.
  7. Di benedetto R, Attorri L, Salvati S, Leonardi F. and Di biase A. 2007. Eicosapentaenoic acid stimulates leptin receptor gene expression in the hypothalamus of newborn rats. Nutr Res 27, 367-371. https://doi.org/10.1016/j.nutres.2007.04.013
  8. Domingo JL, Bocio A, Marti-Cid R and Llobet JM. 2007. Benefits and risks of fish consumption Part II. RIBEPEIX, a computer program to optimize the balance between the intake of Omega-3 fatty acids and chemical contaminants. Toxicology 230, 227-233. https://doi.org/10.1016/j.tox.2006.11.059
  9. Dural M, Ziya Lugal Goksu M and Ozak AA. 2007. Investigation of heavy metal levels in economically important fish species captured from the Tuzla lagoon. Food Chem 102, 415-421. https://doi.org/10.1016/j.foodchem.2006.03.001
  10. Eastwood S and Couture P. 2002. Seasonal variations in condition and liver metal concentrations of yellow perch (Perca flavescens) from a metal-contaminated environment. Aquat Toxicol 58, 43-56. https://doi.org/10.1016/S0166-445X(01)00218-1
  11. Food and Environmental Hygiene Department (FEHD) of the Hong kong Government. 1983. Food Adulteration (Metallic Contamination) Regulations, cap 132V. Laws of Hong Kong, Hong Kong, CN.
  12. Gilbert RJ, Louvois JD, Donovan T, Little C, Nye K, Ribeiro CD, Richards J, Roberts D and Bolton FJ. 2000. Guidelines for the microbiological quality of some ready-to-eat foods sampled at the point of sale. Commun Dis Public Health 3,163-167.
  13. Grigorakis K, Taylor KDA and Alexis MN. 2003. Organoleptic and volatile aroma compounds comparison of wild and cultured gilthead sea bream (Sparus aurata): sensory differences and possible chemical basis. Aquaculture 225, 109-119. https://doi.org/10.1016/S0044-8486(03)00283-7
  14. Harris WS. 1997. n-3 fatty acids and serum lipoproteins human studies. Am J Clin Nutr 65, 1645S-1654S. https://doi.org/10.1093/ajcn/65.5.1645S
  15. Hong SI and Pyun YR. 1999. Inactivation kinetics of Lactobacillus plantarum by high pressure carbon dioxide. J Food Sci 64, 728-733. https://doi.org/10.1111/j.1365-2621.1999.tb15120.x
  16. International Council for the Exploration of the Sea (ICES). 1988. Results of 1985 baseline study of contaminant in fish and shellfish. ICES Coop, Copenhagen, DK.
  17. Isenschimid A, Marison IW and Vonstockar U. 1995. The influence of pressure and temperature of compressed $CO_2$ on the survival of yeast cells. J Biotechnol 39, 229-237. https://doi.org/10.1016/0168-1656(95)00018-L
  18. Jose LD, Ana B, Gemma F and Llobet JM. 2007. Benefits and risks of fish consumption: part I. A quantitative analysis of the intake of omega-3 fatty acids and chemical contaminants. Toxicology 230, 219-226. https://doi.org/10.1016/j.tox.2006.11.054
  19. Josephson DB. 1991. Seafood. In: Volatile Compounds in Foods and Beverages. Maarse H, ed. Dekker Inc., New York, US, pp.179-202.
  20. Josephson DB, Lindsay RC and Stuiber DA.1984. Biogenesis of lipid-derived volatile aroma compounds in the emerald shiner (Notropis atherinoides). J Agric Food Chem 32, 1347-1352. https://doi.org/10.1021/jf00126a032
  21. Kanai G, Kato H, Umeda N, Okada k and Matsuzaki M. 2010. Drying condition and qualities of rapeseed and sunflower. JARQ 44, 173-178. https://doi.org/10.6090/jarq.44.173
  22. Khalifa KM, Hamil AM, Al-Houni AQA and Ackacha MA. 2010. Determination of heavy metals in fish species of the Mediterranean sea (Libyan coast line) using atomic absorption spectrometry. Int J Chem Tech Res 2, 1350-1354.
  23. Kraus-etschmann S, Shadid R, Campoy C, Hoster E, Demmelmair H, Jimenez M, Gil A, Rivero M, Veszpremi B, Decsi T and Koletzko BV. 2007. Effects of fish oil and folate supplementation of pregnant women on maternal and fetal plasma concentrations of docosahexaenoic acid and eicosapentaenoic acid: a European randomized multicenter trial. Am J Clin Nutr 85, 1392-1400. https://doi.org/10.1093/ajcn/85.5.1392
  24. Lee MK, Uddin MS and Chun BS. 2008. Off flavors removal and storage improvement of mackerel viscera by supercritical carbon dioxide extraction. J Environ Biol 29, 591-597.
  25. Methven L, Tsoukka M, Oruna-Concha MJ, Parker JK and Mottram DS. 2007. Influence of sulfur amino acids on the volatile and nonvolatile components of cooked salmon (Salmo salar). J Agric Food Chem 55,1427-1436. https://doi.org/10.1021/jf0625611
  26. Ministry of Public Health, Thailand (MPHT). 1986. Residues in foods. In: Special Issue, 16 February. The Government Gazette, Bangkok, TH, pp.1123-1124.
  27. O'Connor DL, Hall R, Adamkin D, Auestad N, Castillo M, Conner WE, Connor SL, Fitzgerald K, Groh-Wargo S, Hartmann EE, Jacobs J, Janowsky J, Lucas A, Margeson D, Mena P, Neuringer M, Nesin M, Singer L, Stephenson T, Szabo J and Zemon V. 2001. Growth and development in preterm infants fed long-chain polyunsaturated fatty acids: a prospective, randomized controlled trial. Pediatrics 108, 359-371. https://doi.org/10.1542/peds.108.2.359
  28. Peralta RR, Shimoda M and Osajima Y. 1996. Further identification of volatile compounds in fish sauce. J Agric Food Chem 44, 3606-3610. https://doi.org/10.1021/jf9603036
  29. Ping S, Peilong S and Yanjie Y. 2008. Response surface optimiztion of wheat germ oil yield by supercritical carbon dioxide extraction. J Food Bioprod Process 86, 227-231. https://doi.org/10.1016/j.fbp.2007.04.001
  30. Piveteau F, Le Guen S, Gandemer G, Baud J-B, Prost C and Demaimay M. 2000. Aroma of fresh oysters Crassostra gigas: composition and aroma notes. J Agric Food Chem 48, 4851-4857. https://doi.org/10.1021/jf991394k
  31. Roh HS, Park JY, Park SY and Chun BS. 2006. Isolation of off flavors and odors from tuna fish oil using supercritical carbon dioxide. Biotechnol Bioprocess Eng 11, 496-502. https://doi.org/10.1007/BF02932073
  32. Seo YS and Lee JK. 2010. Simultaneous analyses for trace multi-odorous and volatile organic compounds in gas using a triple-bed adsorbent tube. Korean Chem Eng Res 48, 244-252.
  33. Turkmen A, Türkmen M, Tepe Y and Akyurt I. 2005. Heavy metals in three commercially valuable fish species from Iskenderun Bay, northern East Mediterranean Sea, Turkey. Food Chem 91, 167-172. https://doi.org/10.1016/j.foodchem.2004.08.008
  34. USFDA. 1993. Guidance Document for Arsenic in Shellfish. DHHS/PHS/FDA/CFSAN/Office of Seafood, Washington, DC, US.
  35. Yilmaz F, Ozdemir N, Demirak A and Tuna AL. 2007. Heavy metal levels in two fish species Leuciscus cephalus and Lepomis gibbosus. Food Chem 100, 830-835. https://doi.org/10.1016/j.foodchem.2005.09.020