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The influence of Procyanidins, Gallic acid and Theaflavins extracted level when fermented sun-dried salt and green tea

천일염을 이용한 녹차의 발효가 Procyanidins와 Gallic acid 및 Theaflavins 추출량에 미치는 영향

  • Yoon, Hyun (Biomedical Laboratory Science of Hanlyo University) ;
  • Choi, Cheol-Won (Biomedical Laboratory Science of Hanlyo University)
  • 윤현 (한려대학교 임상병리학과) ;
  • 최철원 (한려대학교 임상병리학과)
  • Received : 2014.02.11
  • Accepted : 2014.06.12
  • Published : 2014.06.30

Abstract

This study examined green tea fermented by the addition of sun-dried salt to increase the extraction of Procyanidins and Gallic acid and Theaflavins. An experiment on the change in the amount of the extract according to the saturation degree of sun-dried salt was performed. The sample of fermented only green tea extracted only Gallic acid (0.004 mg/g). No Procyanidins and Theaflavins were extracted. When the green tea was fermented by sun-dried salt, large amounts of the three compounds were extracted: Procyanidins, 75%(0.244 mg/g) and 100%(0.949 mg/g); Gallic acid, 75%(0.386 mg/g) and 100%(0.691 mg/g), and Theaflavins, 75%(0.083 mg/g). According to the date of fermentation, the largest amount of Procyanidins, Gallic acid and Theaflavins extracted were on the 2nd day(0.295 mg/g) and 7th day(2,256 mg/g) and 4th day(0.168 mg/g), respectively.

본 연구는 녹차에서 Procyanidins와 Gallic acid 및 Theaflavins의 추출을 높이기 위해 천일염을 첨가하여 발효시키는 방법에 대하여 실험하였다. 천일염의 포화도에 따른 추출량의 변화에서, 녹차만을 발효한 표본에서는 Gallic acid(0.004 mg/g)만이 추출되었고, Procyanidins와 Theaflavins는 추출되지 않았다. 천일염을 첨가하여 발효한 표본에서는 천일염의 포화도에 따라 Procyanidins는 75% 포화도(0.244 mg/g), 100% 포화도(0.949 mg/g), Gallic acid는 75% 포화도(0.386 mg/g), 100% 포화도(0.691 mg/g), Theaflavins는 75% 포화도(0.083 mg/g)에서 가장 많은 양이 추출되었다. 발효 일에 따른 추출량의 변화에서 Procyanidins는 발효 2일째(0.295 mg/g), Gallic acid는 발효 7일째(2,256 mg/g), Theaflavins는 발효 4일째(0.168 mg/g)에서 가장 많은 양이 추출되었다.

Keywords

References

  1. M. G. Choung, M. S. Lee, "Optimal HPLC condition for simultaneous determination of catechins and caffeine green tea extracts", Korean journal of crop science, Vol.53, No.2, pp.224-232, 2008
  2. K. C. Sung, "A study on the pharmaceutical characteristic and analysis of green tea Extract," J. Korean Oil Chemists's SOC, Vol.23, No.2, pp.115-124, 2006
  3. M. C. Sabu, K. Smitha, K. Ramadas, "Antidiabetic activity of green tea polyphenols and their role in reducing oxidative stress in experimental diabetes," J. Ethanopharmacol, Vol.83, No.2, pp.109-116, 2002 https://doi.org/10.1016/S0378-8741(02)00217-9
  4. C. S. Park, M. S. Cha, "Comparison of antibacterial activities of green tea extracts and preservatives to the pathogenic bacteria," Korean J. Food Nutr., Vol.13, No.1, pp.36-44, 2000
  5. Y. S. Cho, H. S. Kim, S. K. Kim, O. C. Kwon, S. J. Jeong, Y. M. Lee, "Antibacterial and bacterial activity of green tea extracts," J. Korean Tea Soc, Vol.3, No.1, pp.89-103, 1997
  6. S. H. Chung, K. H. Yoon, "Antimicrobial activity of extracts and fractions of green tea used for coarse tea," J. Korean Soc. Food Sci. Nutr., Vol.37, No.11, pp.1382-1388, 2009 DOI: http://dx.doi.org/10.3746/jkfn.2008.37.11.1382
  7. S. T. Kang, U. H. Yoo, K. H. Nam, J. Y. Kang, K. S. Oh, "Antioxidative effects of green tea extract on the oxidation of anchovy oil," J. Agric. Life Sci., Vol.41, No.3, pp.47-53, 2007
  8. K. J. Lee, "Antioxidant activity analysis of catechin compounds in Korean green tea using HPLC online ABTS+ antioxidant screening system," Korean J. Biotechnol. Bioeng. Vol.23, No.1, pp.96-100, 2009
  9. W. O. Choi, J. W. Lee, "Effect of Catechins on Serum Lipids in Obese Women", The Journal of the Korea Contents Association, Vol.12, No.1, pp.338-345, 2012 DOI: http://dx.doi.org/10.5392/JKCA.2012.12.01.338
  10. F. L. Chung, J. Schwartz, C. R. Herzog, Y. M. Yang, "Tea and cancer prevention, Studies in animals and humans," J. Nutr. Vol.133, No.4, pp.3268-3274, 2003 https://doi.org/10.1093/jn/133.10.3268S
  11. D. M. Morre, D. J. Morre, "Anticancer activity of grape and grape skin extracts alone and combined with green tea infusions," Cancer Let,, Vol.238, No.2, pp.202-209, 2006 DOI: http://dx.doi.org/10.1016/j.canlet.2005.07.011
  12. Y. J. Cai, L. P. Ma, L. F. Hou, B. Z. Li, L. Y. Yang, Z. L. Liu, "Antioxidant effects of green tea polyphenols on free radical initiated peroxidation of rat liver microsomes," Chem. Phys. Lipids, Vol.120, No.1/2, pp.109-117, 2002 DOI: http://dx.doi.org/10.1016/S0009-3084(02)00110-X
  13. W. S. Jeong, A. N. Kong, "Biological Properties of Monomeric and Polymeric Catechins: Green Tea Catechins and Procyanidins," Pharmaceutical biology Vol.42, suppl.1, pp.84-93, 2004 DOI: http://dx.doi.org/10.3109/13880200490893500
  14. W. S. Jeong, I. W. Kim, R. Hu, A. N. Kong, "Modulation of AP-1 by chemopreventive agents in human colon HT-29 cancer cell line," Pharmaceutical Research, Vol.24, No.4, pp.649-660, 2004 DOI: http://dx.doi.org/10.1023/B:PHAM.0000022412.69380.d7
  15. W. S. Jeong, I. W. Kim, R. Hu, A. N. Kong, "Modulatory properties of various natural chemopreventive agents of on the activation of NFkB signaling pathway," Pharmaceutical Research, Vol.24, No.4, pp.661-670, 2004 DOI: http://dx.doi.org/10.1023/B:PHAM.0000022413.43212.cf
  16. M. H. Pan, Y. C. Liang, S. Y. Lin-Shiau, N. Q. Zhu, C. T. Ho, J. K. Lin, "Induction of apoptosis by the oolong tea polyphenol theasinensin A though cytochrome c release and activation of caspase-9 and caspase-3 in human U-937 cell," J. Agri, Food Chem, Vol.48, No.12, pp.6337-6346, 2000 DOI: http://dx.doi.org/10.1021/jf000777b
  17. J. V. Higdon, B. Frei, "Tea catechins and polyphenols: health effects, metabolism, and antioxidant functions." Crit. RevFood Sci. & Nutr, Vol.43, No.1, pp.89-143, 2003 DOI: http://dx.doi.org/10.1080/10408690390826464
  18. P. C. Hollman, E. J. Feskens, M. B. Katan, "Tea flavonols in cardiovascular disease and cancer epidemiology," Proceedings of Soc. Exp. Biol. & Med, Vol.220, No,4, pp.198-202, 1999 DOI: http://dx.doi.org/10.3181/00379727-220-44365
  19. O. K. Kim, "Antidiabetic and antioxidative effects of Corni fructus in streptozotocin induced diabetic rats," J. Korean. Oil Chem. Soc, Vol.22, No.2, pp157-167, 2005.
  20. J. W. Jhoo, "Anti-inflammatory Effects of Purpurogallin Carboxylic Acid, An Oxidation Product of Gallic Acid in Fermented Tea," Food science and technology, Vol.40, No.6, pp.707-711, 2008
  21. J. O. Ha, K. Y. Park, "Comparison of mineral contents and external structure of various salts," Korean J. Food Sci. Nutr, Vol.27, No.3, pp.413-418, 1999
  22. M. W. Park, Y. K. Park, "Changes of physicochemical and sensory characteristics of Oiji (Korean pickled cucumbers) prepared with different salts," Korean J. Food Sic. Nutr., Vol.27, No.3, pp.417-424, 1999
  23. M. S. Shin, H. I. Rhee, "The properties of salts and their effects on salted vegetables," Korean Home Econ. Asso, Vol.21, No.1, pp.55-63, 1983
  24. E. S. Maurice, R. Y. Vernon, "Nutrition and diet in hypertension. In Modern Nutrition in Health and Disease," 7th ed. Lea & Febiger, Philadelphia, pp.1272, 1988
  25. S. H. Hwang, "A study on the heavy metal contents of common salts in Korea," Korean J. Environ. Health. Soc., Vol.14, No.1, pp.73-86, 1998
  26. J. O. Ha, K. Y. Park, "Comparison of mineral contents and external structure of various salts," Korean J. Food Sci. Nutr., Vol.27, No.3, pp.413-418, 1998
  27. C. S. Kong, S. S. Bak, K. O. Jung, J. H. Kil, S. Y. Lim, K. Y. Park, "Antimutagenic and anticancer effects of salted maker with various kinds of salts," J. Kor. Fish. Soc., Vol.38, No.5, pp.281-285, 2005
  28. H. Yun, H. J. Oh, S. W. Choi, "Difference of Catechins Extracted Level when Fermented Sun-dried Salt and Green Tea", The Journal of the Korea Contents Association, Vol.12, No.1, pp.278-285, 2012 DOI: http://dx.doi.org/10.5392/JKCA.2012.12.11.278
  29. J. Y. Chang, I. C. Kim, H. C. Chang, "Effect of Solar Salt on the Fermentation Characteristics of Kimchi," Korean J, Food Preserv, Vol.18, No.2, pp.256-265, 2011 https://doi.org/10.11002/kjfp.2011.18.2.256
  30. S. J. Kim, H. L. Kim, K. S. Han, "Characterization of kimchi fermentation prepared with various salt," Korean J, Food Preserv, Vol.12, No.4, pp.395-401, 2005
  31. K. H. Lee, Y. S. Kim, J. Y. Lee, "Changes of Nutrient Composition and Antioxidative Activities of Fermented Tea during Fermentation," korean J. Food & Nutr. Vol.26. No.3, pp.398-403, 2013 DOI: http://dx.doi.org/10.9799/ksfan.2013.26.3.398
  32. J. W. Lee, "Surface design research of functional fermented milk food," The Journal of the Korea Contents Association, Vol.5, No.6, pp.163-171, 2005
  33. S. H. Choi, "Changes in the Composition of Catechins, Theaflavins and Alkaloids in Leaves from Korean Yabukida Tea Plant During Processing to Fermented Black Tea," Korean J. Food Culture, Vol.24, No.3, pp.308-314, 2009
  34. S. V. Jovanovic, Y. Hara, S. Steenken, M. G. Simic, "Antioxidant potential of theaflavins. A pulse radiolysis study," J. Am. Chem. Soc. Vol.119, No.23, pp.5337-5343, 1997 DOI: http://dx.doi.org/10.1021/ja970120f
  35. R. G. Bailey, H. E. Nursten, I. McDowell, "The chemical oxidation of catechins and other phenolics: A study of the formation of black tea pigment," J. Sci. Food Agr. Vol.63, pp.455-464, 1993 DOI: http://dx.doi.org/10.1002/jsfa.2740630413
  36. T. H. Kim, S. K. Ku, I. C. Lee, "Anti-inflammatory functions of purpurogallin in LPS-activated human endothelial cells," BMB reports, Vol.45, No.3, pp.200-205, 2012 https://doi.org/10.5483/BMBRep.2012.45.3.200
  37. Y. H. Kim, Y. K. Lee, S. Y. Choi, K. S. Lee, "Changes Catechins, Caffeine and Gallic acid Contents in Green tea extract by Contamination," The Korean Society of Medicinal Crop Science, May 18, pp.169-170, 2007