Sporulation of Lysinibacillus sphaericus WJ-8 Isolated from Concrete Pavement and Response to Environmental Stresses

콘크리트 포장에서 분리한 Lysinibacillus sphaericus WJ-8의 포자 형성과 환경 스트레스 반응

  • Han, Sang-Hyun (Department of Biological Engineering, Inha University) ;
  • Kang, Chang-Ho (Department of Biological Engineering, Inha University) ;
  • Shin, Yu Jin (Department of Biological Engineering, Inha University) ;
  • Yeom, Woo Sung (Department of Civil Engineering, Inha University) ;
  • Jeong, Jin Hoon (Department of Civil Engineering, Inha University) ;
  • So, Jae-Seong (Department of Biological Engineering, Inha University)
  • Received : 2013.05.20
  • Accepted : 2013.12.17
  • Published : 2014.06.30


Calcite forming bacteria (CFB) have been received increasing attention as a novel and environmental friendly strategy for the healing of concrete crack. Among the CFB, spore forming bacteria were proposed to overcome concrete condition (high pH, hydration heat, deicer). In this study, Lysinibaclillus sphaericus WJ-8 (WJ-8) isolated from concrete pavement was characterized. The WJ-8 was able to precipitate calcite at 10 mg/mL. When observed by scanning electron microscopy, WJ-8 showed spore formation and maximum spore yield was approximately 97.9%. Also response of spores against various environment stresses was examined. Approximately 83~97% of spores maintained their survivability at each three conditions ($60^{\circ}C$, 3 M NaCl and pH 12).


Supported by : 한국연구재단


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