Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Prevalence of Anaplasma sp. in Thrushes (Family Turdidae) in Jeju Island, Republic of Korea

제주도와 마라도내 지빠귀과 조류에서 Anaplasma spp. 감염 조사

  • Oh, Mi-Rae (College of Veterinary Medicine, Jeju National University) ;
  • Moon, Kyoung-Ha (College of Veterinary Medicine, Jeju National University) ;
  • Kim, So-Yeon (College of Veterinary Medicine, Jeju National University) ;
  • Kim, Yun-Gi (College of Veterinary Medicine, Jeju National University) ;
  • Choi, Chang-Yong (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kang, Chang-Wan (Jeju Wildlife Research Center) ;
  • Kim, Hwa-Jung (National Institute of Biological Resources) ;
  • Lee, Kyoung-Kap (College of Veterinary Medicine, Jeju National University) ;
  • Yun, Young-Min (College of Veterinary Medicine, Jeju National University)
  • Accepted : 2014.06.18
  • Published : 2014.06.30
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Abstract

Anaplasmosis is a rickettsial zoonosis mediated by blood-sucking arthropods, such as ticks, flies, and mosquitos. Migratory birds are common hosts of ticks that are mediators of anaplasmosis, in particular, the tick infection rate in thrushes (family Turdidae) has been known to be high. The main purpose of this study is to survey the occurrence and prevalence of Anaplasma spp. from the migratory thrushes in Jeju island. We collected blood samples from 6 thrushes rescued at the Jeju Wildlife Rescue Center and from 34 wild-caught thrushes on Mara island which is a satellite island of Jeju. As a result, the nested PCR confirmed that seven out of 40 individuals (17.5%) were infected by Anaplasma spp. and all of them were identified as A. phagocytophilum based on sequences obtained from partial 16S rRNA. All the infected birds were on their northward migration in spring, our results suggest that the Turdidae family, which is a common and abundant migrant group passing through Jeju island, may act a role as active reservoir and disperser of A. phagocytophilum causing potential influx of the zoonotic pathogens from its wintering grounds in lower latitude to the mainland Korea as well as Jeju.

Anaplasmosis 은 흡혈성 절지동물인 진드기, 이파리, 모기 등에 의해 매개되는 리케차성 인수공통 전염병이다. 철새는 anaplasmosis의 매개체인 진드기의 숙주이다. 제주도의 다양한 철새 분류군 중에서 지빠귀과 조류의 진드기 감염률이 높다. 특히 마라도는 봄철 남방구에서 북방구로 이동하는 철새의 중간기착점으로 지리적으로 중요한 위치에 있다. 따라서 본 연구에서는 제주도의 대표적 이동철새인 지빠귀과 새들의 Anaplasma spp. 감염여부를 조사하였다. 우리는 마라도에서 34마리의 혈액과 제주야생동물구조센터에서 구조 채취된 6개의 혈액 시료를 대상으로 하였다. 그 결과, 40개체의 지빠귀 중 7개체가 감염이 확인되었으며, 감염률은 17.5%로 나타났다. 7개체 모두 Anaplasma phagocytophilum으로 동정되었다. 이러한 결과는 제주도를 통과하는 대표적인 철새인 지빠귀과 새들이 A. phagocytophilum을 육지로 전파할 수 있음을 시사하며, 다른 이동성 철새들간의 질병전파의 보균자로 작용할 수 있기에 철새, 텃새 및 가축으로의 전파여부를 지속적으로 모니터링할 필요가 있다.

Keywords

References

  1. Capligina V, Salmane I, Keiss O, Vilks K, Japina K, Baumanis V, Ranka R. Prevalence of tick-borne pathogens in ticks collected from migratory birds in Latvia. Ticks Tick Borne Dis 2014; 5: 75-81. https://doi.org/10.1016/j.ttbdis.2013.08.007
  2. Chen SM, Dumler JS, Bakken JS, Walker DH. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J Clin Microbiol 1994; 32: 589-595.
  3. Choi CY, Kang CW, Kim EM, Lee S, Moon KH, Oh MR, Yamauchi T, Yun YM. Ticks collected from migratory birds, including a new record of Haemaphysalis formosensis Neumann, on Jeju Island, Korea. Exp Appl Acarol 2014; 62: 557-566. https://doi.org/10.1007/s10493-013-9748-9
  4. De La Fuente J, Naranjo V, Ruiz-Fons F, Hofle U, Fernandez De Mera IG, Villanua D, Almazan C, Torina A, Caracappa S, Kocan KM, Gortazar C. Potential vertebrate reservoir hosts and invertebrate vectors of Anaplasma marginale and A. phagocytophilum in central Spain. Vector Borne Zoonotic Dis 2005; 5: 390-401. https://doi.org/10.1089/vbz.2005.5.390
  5. Dumler JS, Barbet AF, Bekker CP, Dasch GA, Palmer GH, Ray SC, Rikihisa Y, Rurangirwa FR. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol 2001; 51: 2145-2165. https://doi.org/10.1099/00207713-51-6-2145
  6. Hamer SA, Goldberg TL, Kitron UD, Brawn JD, Anderson TK, Loss SR, Walker ED, Hamer GL. Wild birds and urban ecology of ticks and tick-borne pathogens, Chicago, Illinois, USA, 2005-2010. Emerg Infect Dis 2012; 18: 1589-1595. https://doi.org/10.3201/eid1810.120511
  7. Hartwig V, von Loewenich FD, Schulze C, Straubinger RK, Daugschies A, Dyachenko V. Detection of Anaplasma phagocytophilum in red foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) from Brandenburg, Germany. Ticks Tick Borne Dis 2014; 5: 277-280. https://doi.org/10.1016/j.ttbdis.2013.11.001
  8. Hasle G. Transport of ixodid ticks and tick-borne pathogens by migratory birds. Front Cell Infect Microbiol 2013; 3:
  9. Hildebrandt A, Franke J, Meier F, Sachse S, Dorn W, Straube E. The potential role of migratory birds in transmission cycles of Babesia spp., Anaplasma phagocytophilum, and Rickettsia spp. Ticks Tick Borne Dis 2010; 1: 105-107. https://doi.org/10.1016/j.ttbdis.2009.12.003
  10. Hornok S, Kovats D, Csorg T, Meli ML, Gonczi E, Hadnagy Z, Takacs N, Farkas R, Hofmann-Lehmann R. Birds as potential reservoirs of tick-borne pathogens: first evidence of bacteraemia with Rickettsia helvetica. Parasit Vectors 2014; 7: 128. https://doi.org/10.1186/1756-3305-7-128
  11. James MC, Furness RW, Bowman AS, Forbes KJ, Gilbert L. The importance of passerine birds as tick hosts and in the transmission of Borrelia burgdorferi, the agent of Lyme disease: a case study from Scotland. Ibis 2011; 153: 293-302. https://doi.org/10.1111/j.1474-919X.2011.01111.x
  12. Kang JG, Kim HC, Choi CY, Nam HY, Chae HY, Chong ST, Klein TA, Ko S, Chae JS. Molecular detection of Anaplasma, Bartonella, and Borrelia species in ticks collected from migratory birds from Hong-do Island, Republic of Korea. Vector Borne Zoonotic Dis 2013; 13: 215-225. https://doi.org/10.1089/vbz.2012.1149
  13. Keesing F, Hersh MH, Tibbetts M, McHenry DJ, Duerr S, Brunner J, Killilea M, LoGiudice K, Schmidt KA, Ostfeld RS. Reservoir competence of vertebrate hosts for Anaplasma phagocytophilum. Emerg Infect Dis 2012; 18: 2013-2016. https://doi.org/10.3201/eid1812.120919
  14. Kim YH, Kang HM, Kang CW, Kim EM, Kim HJ, Jang YC, Oh HS. Avifauna of Spring season in Marado. Kor J Orni 2010; 17: 27-35.
  15. Machado RZ, Andre MR, Werther K, de Sousa E, Gavioli FA, Alves Junior JR. Migratory and carnivorous birds in Brazil: reservoirs for Anaplasma and Ehrlichia species? Vector Borne Zoonotic Dis 2012; 12: 705-708. https://doi.org/10.1089/vbz.2011.0803
  16. Masuzawa T, Uchishima Y, Fukui T, Okamoto Y, Pan MJ, Kadosaka T, Takada N. Detection of Anaplasma phagocytophilum and Anaplasma bovis in Small Wild Mammals from Taichung and Kinmen Island, Taiwan. Jpn J Infect Dis 2014; 67: 111-114. https://doi.org/10.7883/yoken.67.111
  17. Moon KH, Lee S, Choi CY, Kim SY, Kang CW, Lee KK, Yun YM. Investigation of Theileria sp. From Ticks and Roe Deer (Capreolus pygargus) in Jeju Island. J Vet Clin 2014; 31: 6-10. https://doi.org/10.17555/ksvc.2014.02.31.1.6
  18. Ogden NH, Lindsay LR, Hanincova K, Barker IK, Bigras-Poulin M, Charron DF, Heagy A, Francis CM, O'Callaghan CJ, Schwartz I, Thompson RA. Role of migratory birds in introduction and range expansion of Ixodes scapularis ticks and of Borrelia burgdorferi and Anaplasma phagocytophilum in Canada. Appl Environ Microbiol 2008; 74: 1780-1790. https://doi.org/10.1128/AEM.01982-07
  19. Oh JY, Moon BC, Bae BK, Shin EH, Ko YH, Kin YJ, Park YH, Chae JS. Genetic Identification and Phylogenetic Analysis of Anaplasma and Ehrlichia Species in Haemaphysalis longicornis Collected from Jeju Island, Korea. J Bacteriol Virol 2009; 39: 257-267. https://doi.org/10.4167/jbv.2009.39.4.257
  20. Palomar AM, Santibanez P, Mazuelas D, Roncero L, Santibanez S, Portillo A, Oteo JA. Role of birds in dispersal of etiologic agents of tick-borne zoonoses, Spain, 2009. Emerg Infect Dis 2012; 18: 1188-1191. https://doi.org/10.3201/eid1807.111777
  21. Santos HA, Thome SM, Baldani CD, Silva CB, Peixoto MP, Pires MS, Vitari GL, Costa RL, Santos TM, Angelo IC, Santos LA, Faccini JL, Massard CL. Molecular epidemiology of the emerging zoonosis agent Anaplasma phagocytophilum (Foggie, 1949) in dogs and ixodid ticks in Brazil. Parasit Vectors 2013; 6: 348. https://doi.org/10.1186/1756-3305-6-348
  22. Stuen S, Granquist EG, Silaghi C. Anaplasma phagocytophilum-a widespread multi-host pathogen with highly adaptive strategies. Front Cell Infect Microbiol 2013; 3: 31.