Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지

The Effects of Bambusae caulis in liquamen and Bambusae concretio silicae on Blood Sugar Reduction and Improvement of Peripheral Nerve Function in Diabetic Rats Induced with Streptozotocin

죽력(竹瀝)과 천축황(天竺黃)이 Streptozotocin으로 당뇨가 유발된 백서의 혈당강하 및 말초신경기능회복에 미치는 영향

  • Park, Soo-Gon (Chung Yeon Oriental Clinic) ;
  • Bae, Kil-Joon (Department of Oriental Rehabilitaion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Lee, Ook-Jae (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Kim, Seon-Jong (Department of Oriental Rehabilitaion Medicine, College of Oriental Medicine, Dongshin University) ;
  • Jung, Min-Young (Department of Oriental Medical Ophthalmology, Otolaryngology & Dermatology, College of Oriental Medicine, Dongshin University)
  • 박수곤 (청연한의원) ;
  • 배길준 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 이욱재 (동신대학교 한의과대학 침구의학교실) ;
  • 김선종 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 정민영 (동신대학교 한의과대학 한방안이비인후피부과학교실)
  • Received : 2013.12.18
  • Accepted : 2014.01.03
  • Published : 2014.01.31
⟨ Previous Next ⟩

Abstract

Objectives This study was designed to investigate the effects of Bambusae caulis in liquamen and Bambusae concretio silicae on blood sugar reduction and improvement of peripheral nerve function in diabetic rat models. Methods Diabetic rat models induced by streptozotocin were divided into five groups. We fed experimental group I of rats basal diet and administered normal saline (3 ml, 1 time/1 day) for 6 weeks. We fed experimental group II of rats basal diet and administered Bambusae caulis in liquamen (100 mg/kg, 1 time/1 day) for 6 weeks. We fed experimental group III, IV, V of rats basal diet and administered Bambusae concretio silicae (100 mg/kg, 200 mg/kg, 400 mg/kg once a day) for 6 weeks. We investigated weight and glucose level of rats, and carried out touch test, hot plate test, sensory & motor nerve conduction velocity test and immunohistochemical study after 48 hours, 2 weeks, 4 weeks and 6 weeks. Results 1. The weight of all experimental group was gradually decreased. And glucose level was significantly decreased in the experimental group II, III, IV, V as compared with experimental group I. Especially experimental group II, IV, V were significantly decreased as compared with experimental group III. 2. In the quantitative analysis by touch test and hot plate test, mechanical pain threshold and heat pain threshold were significantly decreased in the other experimental groups as compared with experimental group I. Especially experimental group II, IV, V were significantly decreased as compared with experimental group III. 3. In the sensory and motor nerve conduction velocity test, sensory and motor nerve conduction velocity were significantly increased in the other experimental groups as compared with experimental group I. Especially experimental group II, IV, V were significantly increased as compared with experimental group III. 4. In the substance P immunohistochemical study, experimental group II, IV, V showed strong immune response in spinal cord. Conclusions Bambusae caulis in liquamen and Bambusae concretio silicae were probably useful to treat patients with diabetic peripheral neuropathy.

Keywords

References

  1. 김진호, 이은용, 이청기. 당뇨환자에서의 감각신경전도검사와 체성감각유발전위 검사. 대한재활의학회지. 1987;11 (1):1-9.
  2. Harati Y. Peripheral neuropathy: new concepts and treatment. Neurologs Clinics. 1992;10(3):783-807.
  3. Greene DA, Lattimer SA, Sima AF. Sorbitol, phosphoinositides and sodium-potassium ATPase in the pathogenesis of diabetic complication. The New England Journal of Medicine. 1987;316(10):599-606. https://doi.org/10.1056/NEJM198703053161007
  4. Dyck PJ. Detection, characterization, and staging of polyneuropathy: assessed in diabetes. Muscle & Nerve. 1988;11(1):21-32. https://doi.org/10.1002/mus.880110106
  5. Yorek MA, Dunlap JA, Stefani MR, Davidson EP. Reversal of hyperglycemic-induced defects in myo-inositol metabolism and Na+-K+ pump activity in cultured neuroblastoma cells by normalizing glucose levels. Metabolism. 1993;42(9):1180-9. https://doi.org/10.1016/0026-0495(93)90278-V
  6. 조정구. 당뇨병성 신경병증 병인의 최신지견. 제8차 대한 당뇨병학회 춘계학술대회 초록집. 1994;1:25-33.
  7. A Society for the Research of Neuropathy in Korean Diabetes Association. Clinical practice of diabetic neuropathy. 2nd ed. Seoul:Korean Association. 2007:1-48.
  8. Diabetes. [cited 2010 Nov 9]. Available from: URL: http//www.hira.or.kr/common/dummy.jsp?pgmid=HIRAA021100000000.
  9. Frank RN. The aldose reductase controversy. Diabetes. 1994;43(2):169-72.
  10. Low PA, Suarez GA. Diabetic neuropathies. Baillieres Clin Neurol. 1995;4(3):401-2, 425.
  11. 杜鎬京. 臨床腎系學硏究. 서울:成輔社 1995:539-58.
  12. 양인명. 糖尿病性 神經病症의 관리. 大韓糖尿病學會誌. 1998; 12(1):11-6.
  13. 辛民數. 臨床本草學. 서울:永林社. 1992:128-32, 169, 221, 372-4, 400-6, 509-11.
  14. 장경선, 오영준, 최찬헌, 최건기. 생산공법차이에 따른 죽력의 성분 및 혈당강하효능 비교. 동의생리병리학회지. 2005; 19(1):152-8.
  15. 정찬원, 장경선, 최찬원, 오영준. 대나무숯 제조과정에서 나오는 竹瀝이 Streptozotocin으로 유발된 당뇨 생쥐에 미치는 영향(I). 동의생리병리학회지. 2001;15(1):28-35.
  16. 정기상, 최찬헌, 장경선. 竹瀝이 db/db mouse의 혈당강하에 미치는 영향. 동의생리병리학회지. 2003;17(1):177-82.
  17. 김상수, 고창남, 조기호, 김영석, 배형섭, 이경섭. 竹瀝이 흰쥐의 摘出心臟에 미치는 影響. 경희의대논문집. 1998;14 (1):89-103.
  18. 박경진. 竹瀝의 足三里 藥鍼과 靜脈投與가 LPS誘發 心血循環障碍에 미치는 影響. 동신대학교 대학원 석사학위논문. 2001.
  19. 류주열, 김영균, 권정남. 竹瀝과 生薑汁이 중대뇌동맥 폐쇄에 의한 腦虛血 損傷에 미치는 影響. 대한한의학회지. 2002;23 (3):134-44.
  20. 윤대환, 이봄비, 심인섭, 나창수. 足三里에 시술된 竹瀝藥鍼이 Intraluminal Filament삽입술에 의하여 誘發된 白鼠의 虛血性 局所 腦損傷에 미치는 影響. 대한경락경혈학회지. 2005;22(2):25-41.
  21. 김천중, 정현우, 장경선, 조수인, 김계엽. 대나무 추출액과 죽력의 혼합물이 국소 뇌혈류량 및 평균 혈압에 미치는 영향. 동의생리병리학회지. 2006;20(3):575-80.
  22. 박사현. 竹瀝(竹酢液)經口投與와 肝兪.膽兪 藥鍼이 Alcohol 代謝 및 肝機能에 미치는 影響. 대한약침학회지. 2002;5(1): 81-90.
  23. 이춘우, 두호경. 竹瀝, 竹瀝湯 및 竹瀝薑汁湯이 發熱白鼠의 解熱에 미치는 影響. 경희대학교 한의대 논문집. 1986;9 (1):517-22.
  24. 최찬헌. 생산공정 차이에 따른 竹瀝이 apoptpsis 및 신경세포 보호 효과에 미치는 영향. 동의생리병리학회지. 2007;21 (5):1250-9.
  25. 全國韓醫科大學 本草學敎授 共編著. 本草學. 서울:永林社. 1998:465-6.
  26. 서영준, 정지천. 신경세포에서 天竺黃과 페놀성 물질의 항산화 및 신경보호 효과. 대한한방내과학회지. 2000;21(2): 219-25.
  27. 이우헌, 정지천. 흰쥐 astrocyte에 있어서 amyloid-$\beta$에 의한 독성과 지질과산화에 미치는 天竺黃의 영향. 대한한방내과학회지. 1998;19(2):381-91.
  28. 김성재, 윤철호, 정지천. 마우스의 Calvarial Bone Resorption에 미치는 天竺黃의 영향. 대한한방내과학회지. 2004; 25(3):398-407.
  29. 조기호, 정우상, 이경진. 六味地黃丸으로 호전된 당뇨병성 신경병증 1례. 대한한방내과학회지. 1999;20(1):286-90.
  30. 박지현, 황희정, 김종대, 강석봉. 糖尿病性 神經病症 1例에 대한 臨床的 考察. 대한한의학방제학회지. 2001;9(1):387-95.
  31. 권영구, 최기림, 이진신, 이병철, 안영민, 안세영, 두호경. 補肝湯으로 호전된 당뇨병성 말초신경병증 2례. 대한한의학회지. 2002;23(1):170-6.
  32. 박송기, 권은희, 신현철, 강석봉. 檳蘇散加味方으로 호전된 당뇨병성 말초신경병증 치험1례. 대한한방내과학회지. 2005; 26(4):935-40.
  33. Copray S, Liem R, Brouwer N, Greenhaff P, Habens F, Fernyhough P. Contraction-induced muscle fiber damage is increased in soleus muscle of streptozotocin-diabetic rats and is associated with elevated expression of brain-derived neurotrophic factor mRNA in muscle fibers and activated satellite cells. Exp Neurol. 2000;161(2):597-608. https://doi.org/10.1006/exnr.1999.7306
  34. 김종연, 김용운, 이석강. Streptozotocin-유도 당뇨병 흰쥐에서 수용체 및 수용체 후과정의 인슐린 저항성의 기전. 당뇨병. 1991;15(1):53-62.
  35. Khan GM, Chen SR, Pan HL. Role of primary afferent nerve in allodynia cause by diabetic neuropathy in rat. Neuroscience. 2002;114(2):291-9. https://doi.org/10.1016/S0306-4522(02)00372-X
  36. Sugden D. Psychopharmocological effects of melatonin in mouse and rat. J Pharmacol Exp Ther. 1983;227(3):587-91.
  37. Dumitru E. Electrodiagnostic medicine. 1st ed. Philadelphia: Hanley & Belfus. 1995:821-4, 905-9.
  38. Sugimoto K, Murakawa Y, Sima AA. Diabetic neuropathy: a continuing enigma. Diabetes Metabolism Research & Reviews. 2000;16(6):408-33. https://doi.org/10.1002/1520-7560(200011/12)16:6<408::AID-DMRR158>3.0.CO;2-R
  39. Greene DA, Stevens MJ, Obrosova I, Feldman EL. Glucose-induced oxidative stress and programmed cell death in diabetic neuropathy. Eur J Pharmacol. 1999;375: 217-23. https://doi.org/10.1016/S0014-2999(99)00356-8
  40. Keegan A, Walbank H, Cotter MA, Cameron NE. Chronic vitamin E treatement prevents defective endothelium-dependent relaxation in diabetic rat aorta. Diabetologia. 1995;38:1475-8. https://doi.org/10.1007/BF00400609
  41. 杜鎬京. 東醫腎系學(下). 서울:東洋醫學硏究院. 1993:1167-8.
  42. 姜鍚峯. 糖尿病性 神經症에 대한 治驗 2例. 대한한의학회지. 1992;13(2):22-5.
  43. 李萍. 益氣養陰活血通絡湯治療糖尿病周圍神經病變80例. 西川中醫. 2004;22(4):44.
  44. 商軍科. 裵瑞霞, 楊國春. 白小林. 活血化瘀通絡法治療糖尿 病周圍神經病變32例. 陜西中醫. 2004;25(3):204-5.
  45. 신용식, 김명옥, 김창환, 남문석. 당뇨병성 신경병증에서 신경전도검사와 이학적 변수와의 연관성. 대한재활의학회지. 2009;33(1):112-7.
  46. Chen X, Levine JD. Hyper-responsivity in a subset of C-fiber nociceptors in a model of painful diabetic neuropathy in the rat. Neuroscience. 2001;102:185-92. https://doi.org/10.1016/S0306-4522(00)00454-1
  47. 김동현, 이강건, 박종원, 박태환, 안병철, 권오상. 당뇨병성 신경병증에서 근위부 비골신경전도검사를 이용한 병태생리학적 접근. 대한신경과학회지. 1998;16(2):201-4.
  48. Ferrai-Forcade A, Temesio P, Gomensoro JB. Estudio de la velocidad de conduction nevviso en la diabetic. Acta Neural Lat Amer. 1960;6:43.
  49. Saini AK, Arun KHS, Kaul CL, Sharma SS. Acute hyperglycemia attenuates nerve conduction velocity and nerve blood flow in male Sprague-Dawley rat: reversal by adenosine. Pharmacol Res. 2004;50(6):593-9. https://doi.org/10.1016/j.phrs.2004.04.004
  50. Seybold V, Elde R. Immunohistochemical studies of peptidergic neurons in the dorsal horn of the spinal cord. The Journal of Histochemistry and Cytochemistry Official Journal of the Histochemistry Society. 1980;28(4):367-70. https://doi.org/10.1177/28.4.6154731
  51. Wiesenfeld-Hallin Z. Substance P and somatostatin modulate spinal cord excitability via physiologically different sensory pathways. Brain Research. 1986;372(2):172-5. https://doi.org/10.1016/0006-8993(86)91473-3
  52. Willis WD. Role of neurotransmitters in sensitization of pain responses. Annals of the New York Academy of Sciences. 2001;933:142-56.