Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Biological control of Colletotrichum panacicola on Panax ginseng by Bacillus subtilis HK-CSM-1

  • Ryu, Hojin (Microbial Plant Activation Laboratory, Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Hoon (Bioresource Institute, Herbking Inc.) ;
  • Suh, Dong-Sang (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Jung, Gun Ho (National Institute of Crop Science, Rural Development Administration) ;
  • Park, Kyungseok (Microbial Plant Activation Laboratory, Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Byung Dae (Bioresource Institute, Herbking Inc.)
  • Received : 2012.11.15
  • Accepted : 2014.04.29
  • Published : 2014.07.15
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Abstract

Background: Biological control of plant pathogens using benign or beneficial microorganisms as antagonistic agents is currently considered to be an important component of integrated pest management in agricultural crops. In this study, we evaluated the potential of Bacillus subtilis strain HK-CSM-1 as a biological control agent against Colletotrichum panacicola. Methods: The potential of B. subtilis HK-CSM-1 as a biological control agent for ginseng anthracnose was assessed. C. panacicola was inoculated to ginseng plants and the incidence and severity of disease was assessed to examine the efficacy of the bacterium as a biological control against C. panacicola. Results: Inoculation of Panax ginseng plants with B. subtilis significantly suppressed the number of disease lesions of C. panacicola and was as effective as the chemical fungicide iminoctadine tris(albesilate). The antifungal activity of B. subtilis against C. panacicola was observed on a co-culture medium. Interestingly, treatment with B. subtilis did not significantly affect the diameter of the lesions, suggesting that the mechanism of protection was through the reduction in the incidence of infection related to the initial events of the infection cycle, including penetration and infection via spore germination and appressorium formation rather than by the inhibition of invasive growth after infection. Conclusion: Our results suggest that B. subtilis HK-CSM-1 can be used as an effective and ecologically friendly biological control agent for anthracnose in P. ginseng.

Keywords

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