Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Plant regeneration of Korean wild ginseng (Panax ginseng Meyer) mutant lines induced by ${\gamma}$-irradiation ($^{60}Co$) of adventitious roots

  • Zhang, Jun-Ying (Faculty of Biotechnology, Jeju National University) ;
  • Sun, Hyeon-Jin (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Song, In-Ja (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Bae, Tae-Woong (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kang, Hong-Gyu (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Ko, Suk-Min (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kwon, Yong-Ik (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kim, Il-Woung (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Lee, Jaechun (School of Medicine, Jeju National University) ;
  • Park, Shin-Young (Department of Clinical Pathology, Cheju Halla University) ;
  • Lim, Pyung-Ok (Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology (DGIST)) ;
  • Kim, Yong Hwan (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET)) ;
  • Lee, Hyo-Yeon (Faculty of Biotechnology, Jeju National University)
  • Received : 2013.12.13
  • Accepted : 2014.04.09
  • Published : 2014.07.15
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Abstract

An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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