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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Immunostimulating activity of maysin isolated from corn silk in murine RAW 264.7 macrophages

  • Lee, Jisun (Department of Biotechnology, The Catholic University of Korea) ;
  • Kim, Sun-Lim (National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Seul (Department of Biotechnology, The Catholic University of Korea) ;
  • Chung, Mi Ja (Department of Food Science and Nutrition, College of Health, Welfare and Education, Gwangju University) ;
  • Park, Yong Il (Department of Biotechnology, The Catholic University of Korea)
  • Received : 2013.08.22
  • Accepted : 2013.10.29
  • Published : 2014.07.31
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Abstract

Corn silk (CS) has long been consumed as a traditional herb in Korea. Maysin is a major flavonoid of CS. The effects of maysin on macrophage activation were evaluated, using the murine macrophage RAW 264.7 cells. Maysin was isolated from CS by methanol extraction, and preparative $C_{18}$ reverse phase column chromatography. Maysin was nontoxic up to $100{\mu}g/ml$, and dose-dependently increased TNF-${\alpha}$ secretion and iNOS production by 11.2- and 4.2-fold, respectively, compared to untreated control. The activation and subsequent nuclear translocation of NF-${\kappa}B$ was substantially enhanced upon treatment with maysin ($1-100{\mu}g/ml$). Maysin also stimulated the phosphorylation of Akt and MAPKs (ERK, JNK). These results indicated that maysin activates macrophages to secrete TNF-${\alpha}$ and induce iNOS expression, via the activation of the Akt, NF-${\kappa}B$ and MAPKs signaling pathways. These results suggest for the first time that maysin can be a new immunomodulator, enhancing the early innate immunity.

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References

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