Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Effects of Dietary Fiber from Mozuku (Cladosiphon novae-caledoniae kylin) Residue on Antioxidant Activity and Anticancer in HT-29 Human Colon Cancer Cells according to Extraction Condition

추출법에 따른 큰실말(Cladosiphon novae-caledoniae kylin) 부산물 식이섬유의 항산화 효과 및 HT-29 대장암 세포에 대한 항암 작용

  • Jeong, Haeng Soon (Department of Pharmaceutical Engineering, Silla University) ;
  • Lee, Jae-Hwa (Department of Pharmaceutical Engineering, Silla University)
  • 정행순 (신라대학교 의생명과학대 생명공학과) ;
  • 이재화 (신라대학교 의생명과학대 생명공학과)
  • Received : 2014.04.04
  • Accepted : 2014.05.07
  • Published : 2014.08.10
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Abstract

In this study, the dietary fiber contents of Mozuku (Cladosiphon novae-caledoniae kylin) residue and the extraction condition (HCl, $H_2SO_4$, NaOH, $Na_2CO_3$, $Na_2EDTA$) of the dietary fiber was investigated. We examined that the contents of the total polyphenols and flavonoids in the dietary fiber from Mozuku residue, and the potent anti-cancer effect was also tested through the growth inhibition in human colon cancer cells (HT-29) in vitro. It was effective to extract soluble dietary fiber with 1.5% $Na_2EDTA$ and 0.05 N HCl in Mozuku residue. The extraction time and temperature affected the yields of soluble dietary fiber. The contents of the total polyphenols and flavonoids in the dietary fiber from Mozuku residue were the highest in 1% NaOH extract (Total polyphenols $34.4{\pm}0.055$ mg gallic acid/g dry basis, total flavonoids $34.7{\pm}0.023$ mg naringin/g extract dry basis). In DPPH radical scavenging activity, 1% NaOH extract showed the most potent antioxidant activity. In the result of viability in human colon cancer cells, growth inhibition was observed in D.W., 0.05 N HCl, and 0.5% $Na_2CO_3$ extracts in a dose-dependent manner. These results demonstrated that soluble dietary fiber from Mozuku residue significant antioxidant activity and anticancer in human colon cancer.

본 연구에서는 큰실말 부산물의 추출 조건(HCl, $H_2SO_4$, NaOH, $Na_2CO_3$, $Na_2EDTA$)에 따른 식이섬유 함량을 조사하였다. 또한 큰실말 부산물로부터 분리한 식이섬유의 총 폴리페놀과 플라보노이드양을 측정하고 HT-29 대장암 세포에서의 증식 억제를 통한 항암효과를 확인하였다. 1.5% $Na_2EDTA$와 0.05 N HCl이 큰실말 부산물로부터 가용성 식이섬유를 추출하는데 효과적이었으며 추출 시간과 온도도 가용성 식이섬유의 추출수율에 영향을 주었다. 큰실말 부산물 식이섬유의 총 폴리페놀과 플라보노이드 함량은 1% NaOH 추출물에서 가장 높게 측정되었다(총 폴리페놀 $34.4{\pm}0.055$ mg gallic acid/g dry basis, 총 플라보노이드 $34.7{\pm}0.023$ mg naringin/g extract dry basis). 또한 DPPH 라디칼 소거능 측정에서도 1% NaOH 추출물에서 가장 높은 항산화 효과를 보였다. 대장암 세포주의 세포 생존율 측정 결과, D.W.와 0.05 N HCl 및 0.5% $Na_2CO_3$ 추출물에서 농도의존적 세포 증식 억제를 보였다. 이들 결과는 큰실말 부산물로부터 분리한 가용성 식이섬유가 항산화 및 대장암에서의 항암효과에 중요한 기능이 있음을 시사한다.

Keywords

References

  1. S. Reiser, Metabolic effects of dietary pectins related to human health, Food Technol., 41, 91-99 (1987).
  2. Y. H. Kim, Analysis of estimated dietary fiber intake of Korean during the 2000s, M.S. Thesis, Kyungpook National University, Kyungpook, Korea (2011).
  3. S. Mabeau and J. Fleurence, Seaweed in food products: biochemical and nutritional aspects, Trends Food Sci. Technol., 4, 103-107 (1993). https://doi.org/10.1016/0924-2244(93)90091-N
  4. M. Lahaye and B. kaeffer, Seaweed dietary fibres: structure, physicochemical and biological properties relevant to intestinal physiology, Sci. Aliments., 17, 563-584 (1997).
  5. B. Kloareg and R. S. Quatrano, Structure of the cell walls of marine algae and ecophysiological functions of the matrix polysaccharides, Oceanogr. Mar. Biol. Annu. Rev., 26, 259-315 (1988).
  6. C. Bobin-Dubigeon, C. Hoebler, V. Lognone, C. Dagorn-Scaviner, S. Mabeau, J.-L. Barry, and M. Lahaye, Chemical composition, physicochemical properties, enzymatic inhibition and fermentative characteristics of dietary fibres from edible seaweeds, Sci. aliments., 17, 619-639 (1997).
  7. C. Michel, M. Lahaye, C. Bonnet, S. Maheau, and J.-L. Barry, In vitro fermentation by human faecal bacteria of total and purified dietary fibres from brown seaweeds, BR. J. Nutr., 75, 263-280 (1996). https://doi.org/10.1079/BJN19960129
  8. B. Kloareg and R. S. Quatrano, Structure of the cell walls of marine algae and ecophysiological functions of the matrix polysaccharides, Oceanogr. Mar. Biol. Annu. Rev., 26, 259-315 (1988).
  9. E. Percival and R. H. Mcdowell, Chemistry and enzymology of marine algal polysaccharides, Academic Press, London, UK (1967).
  10. Y. S. Lee, D. S. Kim, B. H. Ryu, and S. H. Lee, Antitumor and immunomodulating effects of seaweeds toward sarcoma-180 cell, J. Kor. Soc. Food Nutr., 21, 544-550 (1992).
  11. K. J. Cho, Y. S. Lee, and B. H. Ryu, Antitumor effect and immunology activity of seaweeds toward sarcoma-180, Bull. Kor. Fish. Soc., 23, 345-352 (1990).
  12. M. Scot, G. M. Colin, J. David, L. Mills, and J. B. Brian, Estimation of meiobenthic nematode diversity by non specialists, Marine Pollu. Bulletin., 18, 646-649 (1987). https://doi.org/10.1016/0025-326X(87)90398-5
  13. E. Furusawa and S. Furusawa, Anticancer potential of viva-natural, a dietary seaweed extract, on lewis lung carcinoma in comparison with chemical immunomodulators and on cyclosporine-accelerated AKR leukemia, Oncology, 46, 343-348 (1989). https://doi.org/10.1159/000226746
  14. Y. Aisa, Y. Miyakawa, T. Nakazato, H. Shibata, K. Saito, Y. Ikeda, and M. Kizaki, Fucoidan induces apoptosis of human HS-sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathway, Am. J. Hematol., 78, 7-14 (2005). https://doi.org/10.1002/ajh.20182
  15. I. Jaswir and H. A. Monsur, Anti-inflammatory compounds of macro algae origin: A review, J. Med. Plants Res., 5, 7146-7154 (2011).
  16. J. Ye, Y. Li, K. Teruya, Y. Katakura, A. Ichikawa, H. Eto, M. Hosoi, M. Hosoi, S. Nishimoto, and S. Shirahata, Enzyme-digested fucoidan extracts derived from seaweed Mozuku of Cladosiphon novae-caledoniae kylin inhibit invasion and angiogenesis of tumor cells, Cytotechnology, 47, 117-126 (2005). https://doi.org/10.1007/s10616-005-3761-8
  17. H. Kawamoto, Y. Miki, T. Kimura, K. Tanaka, T. Nakagawa, M. Kawamukai, and H. Matsuda, Effects of fucoidan from Mozuku on human stomach cell lines, Food Sci. Technol. Res., 12, 218-222 (2006). https://doi.org/10.3136/fstr.12.218
  18. T. Kuda, M. Tsunekawa, H. Goto, and Y. Araki, Antioxidant properties of four edible algae harvested Noto Peninsula, Japan, J. of Food Compos. Anal., 18, 625-633 (2005). https://doi.org/10.1016/j.jfca.2004.06.015
  19. J. Shimizu, U. Wada-Funada, H. Mano, Y. Matahira, M. Kawaguchi, and M. Wada, Proportion of murine cytotoxic T cells is increased by high molecular-weight fucoidan extracted from Okinawa mozuku (Cladosiphon okamuranus), J. Health Sci., 51, 394-397 (2005). https://doi.org/10.1248/jhs.51.394
  20. C. C. Chang, M. H. Yang, H. M. Wen, and J. C. Chen, Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J. Food Drug Anal., 10, 178-182 (2002).
  21. V. L. Singleton and J. A. Rossi, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, J. Enol. Viticult., 16, 144-158 (1965).
  22. J.-R. Do, E.-M. Kim, J.-G. Koo, and K.-S. Jo, Dietary fiber contents of marine algae and extraction condition of the fiber, J. Korean Fish. Soc., 30, 291-296 (1997).
  23. H. S. Son, Method for preparing insoluble fiber from soy, Korea Patent, 10-0785367 (2007).
  24. Y.-R. Kwon and K.-S. Youn, Quality and antioxidant characteristics of granule tea prepared with sea tangle (Laminaria japonica) and sea mustard (Undaria pinnatifida) powder as affected by extraction method, Korean J. Food Preserv., 19, 525-531 (2012). https://doi.org/10.11002/kjfp.2012.19.4.525
  25. N.-Y. Park, I. S. Kim, and Y.-J. Jeong, Effects of extraction conditions on the componential extraction of brown seaweed (Undaria pinnatifida), J. Food Sci. Nutr., 13, 321-326 (2008). https://doi.org/10.3746/jfn.2008.13.4.321

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