Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Free radical scavenging, anti-inflammatory and melannin synthesis inhibitory activities of Gloeostereum incarnatum

느릅나무버섯 자실체의 메탄올과 열수추출물의 항산화, 항염증 및 멜라닌 생성저해 활성

  • 권예주 (인천논현고등학교) ;
  • 김미현 (인천논현고등학교) ;
  • 최재순 (인천논현고등학교) ;
  • 이태수 (인천대학교 생명과학기술대학, 생명과학부)
  • Received : 2014.05.29
  • Accepted : 2014.06.25
  • Published : 2014.06.30
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Abstract

Gloeostereum incarnatum is an edible and medicinal mushroom belongs to Family Cyphellaceae of Polyporales, Basidiomycota. The purpose of this study was to investigate the free radical scavenging, anti-inflammatory, and melanin synthesis inhibitory activities of fruiting bodies of Gloeostereum incarnatum. In the free radical scavenging activities, the mushroom extracts showed good 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and chelating activity on the ferrous ions compared with the positive control, BHT. The mushroom extract suppressed nitric oxide (NO) production in LPS-induced RAW 264.7 macrophage cells in dose dependant manners. Significant reduction of paw edema of rats were observed at 2~6 h after administration with 50 mg/kg of the methanol and hot-water extracts, which were comparable with treatment of 5 mg/kg of indomethacin, the positive control. The melanin synthesis of Melanoma B16/F10 cells treated with $100{\mu}g/mL$ of the methanol and hot water extracts decreased melanin concentration to 50% and 45% compared with the control, arbutin. Therefore, the experimental results showed that methanol and hot-water extracts of Gloeostereum incarnatum fruiting bodies might be used for good sources of anti-inflammatory, free radical scavenging, and skin whitening agents for human health.

본 연구에서는 느릅나무버섯의 자실체에서 메탄올과 열수를 이용해 추출한 물질의 항산화, 항염증 및 Melanoma B16/F10 세포에서의 멜라닌 생성 저해 효과를 탐색하였다. DPPH 라디칼 소거능과 환원력을 이용해 항산화 효과를 측정한 결과 양성대조군으로 사용한 BHT에 비해 항산화 효과는 낮았지만 다른 종류의 버섯에 비해 효과가 우수한 것으로 나타났으며, 철 이온 제거 항산화 실험에서 느릅나무버섯의 열수 추출물은 양성대조군인 BHT에 비해 철 이온을 효율적으로 제거하여 항산화 효과가 높게 나타났다. 느릅나무버섯 자실체의 염증 저해 실험에서는 배양 중인 RAW 264.7 대식세포에 자실체의 메탄올과 열수추출물을 각각 처리 한 후 염증 유도 물질인 LPS를 투여하여 추출물의 NO 생성 저해효과를 조사한 결과, 처리한 추출물의 농도 의존적으로 염증 유발의 주요 인자인 NO의 양이 현저하게 감소하는 우수한 소염효과를 보였으며, carrageenan에 의해 흰쥐 뒷발에 유도된 부종을 저해하는 실험에서는 투여한 추출물의 농도가 증가함에 따라 흰쥐의 뒷발에 유도된 부종의 용적도 비례적으로 감소하여 항염증 효과가 높은 것으로 나타났다. Melanoma 세포에 버섯추출물을 처리하고 생합성된 멜라닌의 양을 측정한 결과 농도 의존적으로 melanoma 세포내 멜라닌의 양이 감소하여 멜라닌 저해 효과가 높은 것으로 나타났다. 따라서 느릅나무버섯 자실체의 항산화, 항염증 및 멜라닌 생성 저해 선분은 앞으로 항산화제, 소염제 및 미백제로의 이용이 가능할 것으로 사료된다.

Keywords

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