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Time-course Analysis of Biofilm Formation in Quorum Sensing-deficient Bacteria

Quorum sensing 결핍 세균에서 생물막 형성의 시간적 추이 분석

  • Kim, Soo-Kyoung (Department of Pharmacy, College of Pharmacy, Pusan National University) ;
  • Lee, Mi-Nan (Department of Pharmacy, College of Pharmacy, Pusan National University) ;
  • Lee, Joon-Hee (Department of Pharmacy, College of Pharmacy, Pusan National University)
  • 김수경 (부산대학교 약학대학 약학과 미생물학 연구실) ;
  • 이미난 (부산대학교 약학대학 약학과 미생물학 연구실) ;
  • 이준희 (부산대학교 약학대학 약학과 미생물학 연구실)
  • Received : 2014.05.07
  • Accepted : 2014.06.05
  • Published : 2014.06.30

Abstract

Pseudomonas aeruginosa and Vibrio vulnificus are Gram-negative human pathogens, which exert their virulence through quorum sensing (QS) regulation. The infection of these pathogens have been known to be mediated by biofilm formation in many cases and this study carried out the time-course analysis of biofilm formation depending on the QS regulation in P. aeruginosa and V. vulnificus. In P. aeruginosa, our results demonstrated that QS-deficient mutant better attached to surface at initial stage of biofilm formation, but poorly proceeded to the maturation of the biofilm structure, while wild type less attached at initial stage but developed highly structured biofilm at late stage. Because of this, the quantitative comparison of biofilm formation between wild type and the QS mutant showed the reversion; the QS mutant formed more biofilm until 10 h after inoculation than wild type, but wild type formed much more biofilm after 10 h than QS mutant. V. vulnificus has been reported to form more biofilm with the mutation on QS system. When we performed the same time-course analysis of the V. vulnificus biofilm formation, the reversion was not detected even with prolonged culture for 108 h and the QS mutant always forms more biofilm than wild type. These results indicate that the QS regulation negatively affects the attachment at early stage but positively facilitates the biofilm maturation at late stage in P. aeruginosa, while the QS regulation has a negative effect on the biofilm formation throughout the biofilm development in V. vulnificus. Based on our results, we suggest that the developmental stage of biofilm and bacterial species should be considered when the QS system is targeted for biofilm control.

Acknowledgement

Supported by : 부산대학교

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