한국조리학회지 (Culinary science and hospitality research)

  • 제20권2호
  • /
  • Pages.16-26
  • /
  • 2014
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  • 2466-0752(pISSN)
  • /
  • 2466-1023(eISSN)
한국조리학회 (Culinary Society of Korean Academy)
권호 표지

적양배추 추출물의 항산화 및 항염증 활성

Antioxidant and Anti-inflammation Activity of Red Cabbage Extract

  • 하현주 (동아대학교 식품영양학과) ;
  • 이춘복 (영산대학교 한국식품조리학과)
  • Ha, Hyun-Joo (Department of Food Science & Nutrition, Dong-A University) ;
  • Lee, Chun-Bok (Department of Korean Food & Culinary Arts, Youngsan University)
  • 투고 : 2013.11.06
  • 심사 : 2014.03.10
  • 발행 : 2014.04.30
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초록

본 연구에서는 생리활성이 우수한 것으로 알려진 적양배추를 이용하여 에탄올 및 열수 추출을 하여 마크로파지 세포에 LPS로 유도하여 염증성 질환 모델에서 세포생존, NO생성 억제, 단백질 단계에서 iNOS와 COX-2의 발현을 분석하였고 항산화 활성 측정을 위해 ABTS 라디칼 소거능과 FRAP 활성을 확인하였다. 각 추출물의 세포생존, NO생성 및 단백질 발현 분석에서 농도 의존적으로 LPS에 대해 억제 및 저해 효능을 확인하였고 특히 0.25, 0.5 그리고 1.0 mg/mL에서 유의적인 차이를 확인하였다. ABTS 라디칼 소거 및 FRAP 활성은 합성 항산화제인 BHT보다 활성이 좋지 않았지만 천연 항산화제로써의 가능성을 확인하였고 훌륭한 식품소재로서 기능성 식품 및 각종 질병에 대한 예방의 목적으로 활용이 가능할 것으로 사료된다.

This study investigated the anti-inflammatory and antioxidnat effects of red cabbage extracts on RAW264.7 cells. Cell toxicity was determined by MTT assay. We evaluated the anti-inflammatory effects of red cabbage extracts by measuring nitric oxide (NO), inducible NOS (iNOS) production, and cyclooxygenase-2 (COX-2) expression by Western blotting. Ethanolic and water extracts (0.25, 0.5, and 1.0 mg/mL) significantly suppressed LPS-stimulated production of NO. Two kinds of extracts reduced the expression of iNOS and COX-2 proteins. The present results show that red cabbage extract has potent anti-inflammatory effects on RAW264.7 cells. In addition, two kinds of extracts as well as various antioxidant activities such as 2,2'-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic acid)(ABTS) radical scavenging activity, ferric reducing antioxidant power(FRAP). The total polyphenol and flavonoid contents of the ethanolic and water extracts from red cabbage were $18.699{\pm}0.87$ and $11.174{\pm}4.86$ mg GAE/g extract, respectively, and $7.782{\pm}2.23$ and $15.608{\pm}3.54$ mg CE/g extract. The ABTS radical scavenging activities of the ethanolic and water extracts and BHT were $0.269{\pm}0.12$, $0.212{\pm}0.22$ and $1.235{\pm}0.07mM$ Trolox equivalent/mg extract, respectively. The FRAP values of the extracts were similar to those of BHT, which were used as a positive control. Therefore, red cabbage extract is considered as a good food material of functional foods for prevention against various diseases.

키워드

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