The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Expression of colSR Genes Increased in the rpf Mutants of Xanthomonas oryzae pv. oryzae KACC10859

  • Noh, Young-Hee (Department of Plant Medicine, Chungbuk National University) ;
  • Kim, Sun-Young (Department of Microbiology, Pusan National University) ;
  • Han, Jong-Woo (Watermelon Research Institute, Chungbuk ARES) ;
  • Seo, Young-Su (Department of Microbiology, Pusan National University) ;
  • Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
  • Received : 2013.12.29
  • Accepted : 2014.05.25
  • Published : 2014.09.01
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Abstract

The rpf genes and $colS_{XOO1207}/colR_{XOO1208}$ were known to require for virulence of Xanthomonas oryzae pv. oryzae (Xoo). In Xoo KACC10331 genome, two more colS/colR genes, $colS_{XOO3534}$ (raxH)/$colR_{XOO3535}$ (raxR) and $colS_{XOO3762}/colR_{XOO3763}$ were annotated. The $colS_{XOO3534}/colR_{XOO3535}$ were known to control AvrXa21 activity and functions of $colS_{XOO3762}/colR_{XOO3763}$ were unknown in Xoo. To characterize the relationship between rpf and colS/colR genes, expression of colS/colR genes in Rpf mutants of Xoo were analyzed with quantitative reverse transcription PCR (qRT-PCR). Expressions of all three colS/colR genes increased in the rpfF mutant in which DSF synthesis is defective. Expression of $colS_{XOO1207}/col-R_{XOO1208}$, $colS_{XOO3534}/colR_{XOO3535}$ and $colS_{XOO3762}/colR_{XOO3763}$ increased 2, 2-7, 3-13 folds respectively. Expression of $colS_{XOO3534}$ and $colS_{XOO3762}$ also increased 2-4 folds in the rpfG mutant in which the signal from DSF is no longer transferred to down-stream. Expression of the other colS/colR genes was not significantly changed in the rpfG mutant compared to the wild type. Since RpfF and RpfG are responsible for DSF synthesis and signal transfer from DSF to down-stream to regulate virulence gene expression, these results suggest that the DSF and DSF-mediated signal regulate negatively three colS/colR genes in Xoo.

Keywords

References

  1. Barber, C. E., Tang, J. L., Feng, J. X., Pan, M. Q., Wilson, T. J. G., Slater, H., Dow, J. M., Williams, P. and Daniels, M. J. 1997. A novel regulatory system required for pathogenicity of Xanthomonas campestris is mediated by a small diffusible signal molecule. Mol. Microbiol. 24:555-566. https://doi.org/10.1046/j.1365-2958.1997.3721736.x
  2. Burdman, S., Y. Shen, S.-W. Lee, Q. Xue, and P. Ronald. 2004. RaxH/RaxR: a two-component regulatory system in Xanthomonas oryzae pv. oryzae required for AvrXa21 activity. Mol. Plant-Microbe Interact. 17:602-612. https://doi.org/10.1094/MPMI.2004.17.6.602
  3. Chatterjee, S. and Sonti, R. 2002. rpF mutants of Xanthomonas oryzae pv. oryzae are deficient for virulence and growth under low iron conditions. Mol. Plant-Microbe Interact. 15:463-471. https://doi.org/10.1094/MPMI.2002.15.5.463
  4. Dekkers, L., Bloemendaal, C. J., Weger, L., Wijffelman, C., Spaink, H. and Lugtenberg, B. J. 1998. A two-component system plays an important role in the root-colonizing ability of Pseudomonas fluorescens strain WCS365. Mol Plant- Microbe Interact. 11:45-56. https://doi.org/10.1094/MPMI.1998.11.1.45
  5. He, Y.-W., Ng, A. Y.-J., Xu, M., Lin, K., Wang, L.-H., Dong, Y.- H. and Zhang, L.-H. 2007. Xanthomonas campestris cell- cell communication involves a putative nucleotide receptor protein Clp and a hierarchical signalling network. Mol. Microbiol. 64:281-292. https://doi.org/10.1111/j.1365-2958.2007.05670.x
  6. He, Y.-W., Wu, J., Cha, J.-S. and Zhang, L.-H. 2010. Rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae produces multiple DSF-family signals in regulation virulence factor production. BMC Microbiol. 10:187. https://doi.org/10.1186/1471-2180-10-187
  7. Hu, N. and Zhao, B. 2007. Key genes involved in heavy-meral resistance in Pseudomonas putida CD2. FEMS Microbial. Lett. 267:17-22. https://doi.org/10.1111/j.1574-6968.2006.00505.x
  8. Jeong, K. S., Lee, S. E., Han, J. W., Yang, S. U., Lee, B. M., Noh, T. H. and Cha, J. S. 2008. Virulence reduction and differing regulation of virulence genes in rpf mutants of Xanthomonas oryzae pv. oryzae. Plant Pathol. J. 24:143-151. https://doi.org/10.5423/PPJ.2008.24.2.143
  9. Kivistik, P. A., Putrins, M., Puvi, K., Ilves, H., Kivsaar, M. and Horak, R. 2006. The ColR two-component system regulates membrane functions and protects Pseudomonas putida against phenol. J. Bacteriol. 188:8109-8117. https://doi.org/10.1128/JB.01262-06
  10. Loper, J. E and Lindow, S. E. 1994. A biological sensor for iron availability to bacteria in their habitats in their plant surfaces. Appl. Environ. Microbiol. 60:1934-1941.
  11. Lee, N. M., Park, Y. J., Park, D. S., Kang, H. W., Kim, J. G., Song, E. S., Park, I. C., Yoon, U. H., Hahn, J. H., Koo, B. S., Lee, G. B., Kim, H., Park, H. S., Yoon, K. O., Kim, J. H., Jung, C. H., Koh, N. H., Seo, J. S. and Go, S. J. 2005. The genome sequence of Xanthomonas oryzae pathover oryzae KACC10331, the bacterial blight pathogen of rice. Nucleic Acids Res. 33:577-586. https://doi.org/10.1093/nar/gki206
  12. Lee, S.-W., Jeong, K.-S., Han, S.-W., Lee, S.-E., Phee, B.-K, Hahn, T.-R. and Ronald, P. 2008. The Xanthomonas oryzae pv. oryzae PhoPQ two-component system is required for AvrXA21 Activity, hrpG expression, and virulence. J. Bacteriol. 190:2183-2197. https://doi.org/10.1128/JB.01406-07
  13. Ryan, R. P., Fouhy, Y., Lucey, J. F., Jiang, B.-L., He, Y.-Q., Feng, J.-X., Tang, J.-L. and Dow, J. M. 2007. Cyclic di-GMP signalling in the virulence and environmental adaptation of Xanthomonas campestris. Mol. Microbiol. 63:429-442. https://doi.org/10.1111/j.1365-2958.2006.05531.x
  14. Seo, Y.-S., Sriariyanun, M., Wang, L., Pfeiff, J., Phetsom, J., Lin, Y., Jung, K.-H., Chou, H. H., Bogdanove, A. and Ronald, P. 2008. A two-genome microarray for the rice pathogens Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola and its use in the discovery of a difference in their regulation of hrp genes. BMC Microbiol. 8:99. https://doi.org/10.1186/1471-2180-8-99
  15. Slater, H., Alvarez-Morales, A., Barber, C. E., Daniels, M. J. and Dow, J. M. 2000. A two-component system involving an HDGYP domain protein links cell-cell signalling to pathogenicity gene expression in Xanthomonas campestris. Mol. Microbiol. 38:986-1003.
  16. Subramoni, S., Pandey, A., Priya, M. R. V., Patel, H. K. and Sonti, R. 2012. The ColRS system of Xanthomonas oryzae pv. oryzae is required for virulence and growth in iron-limiting conditions. Mol. Plant Pathol. 13:690-703. https://doi.org/10.1111/j.1364-3703.2011.00777.x
  17. Tang, J. L., Liu, Y. N., Barber, C. E., Dow, J. M., Wootton, J. C. and Daniels, M. J. 1991. Genetic and molecular analysis of a cluster of rpf genes involved in positive regulation of synthesis of extracellular enzymes and polysaccharide in Xanthomonas campestris pathovar campestris. Mol. Gen. Genet. 226:409-417.
  18. Wang, L. H., He, Y. W., Gao, Y. F., Wu, J. E., Dong, Y. H., He, C. Z., et al. 2004. A bacterial cell-cell communication signal with cross-kingdom structural analogues. Mol. Microbiol. 51: 903-912.
  19. Yan, Q. and Wang, N. 2011. The ColR/ColS two-component system plays multiple roles in the pathogenicity of the citrus canker pathogen Xanthomonas citir subsp. citiri. J. Bactriol. 193:1590-1599. https://doi.org/10.1128/JB.01415-10
  20. Zhang, S. S., He, Y. Q., Xu, L. M., Chen, B. W., Jiang, B. L., Liao, J., Cao, J. R., Liu, D., Huang, Y. Q., Liang, X. X., Tang, D. J., Lu, G. T. and Tang, J. L. 2008. A putative colR(XC1049)-colS(XC1050) two-component signal transduction system of Xanthomonas campestris positively regulates hrpC and hrpE operons and is involved in virulence, the hypersensitive response and tolerance to various stresses. Res. Microbiol. 159:569-578. https://doi.org/10.1016/j.resmic.2008.06.010

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