Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Kinetic Characterization and Molecular Modeling of $NAD(P)^+$-Dependent Succinic Semialdehyde Dehydrogenase from Bacillus subtilis as an Ortholog YneI

  • Park, Seong Ah (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Park, Ye Song (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan) ;
  • Lee, Ki Seog (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
  • Received : 2014.02.25
  • Accepted : 2014.05.06
  • Published : 2014.07.28
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Abstract

Succinic semialdehyde dehydrogenase (SSADH) catalyzes the oxidation of succinic semialdehyde (SSA) into succinic acid in the final step of ${\gamma}$-aminobutyric acid degradation. Here, we characterized Bacillus subtilis SSADH (BsSSADH) regarding its cofactor discrimination and substrate inhibition. BsSSADH showed similar values of the catalytic efficiency ($k_{ca}t/K_m$) in both $NAD^+$ and $NADP^+$ as cofactors, and exhibited complete uncompetitive substrate inhibition at higher SSA concentrations. Further analyses of the sequence alignment and homology modeling indicated that the residues of catalytic and cofactor-binding sites in other SSADHs were highly conserved in BsSSADH.

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References

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