DOI QR코드

DOI QR Code

Optimum Conditions for Extracting Flavanones from Grapefruit Peels and Encapsulation of Extracts

자몽껍질 유래 플라바논의 최적 추출 및 기능성 소재 캡슐화

  • Ko, Min-Jung (Department of Food Science and Engineering, Ewha Womans University) ;
  • Kwon, Hye-Lim (Department of Food Science and Engineering, Ewha Womans University) ;
  • Chung, Myong-Soo (Department of Food Science and Engineering, Ewha Womans University)
  • 고민정 (이화여자대학교 식품공학과) ;
  • 권혜림 (이화여자대학교 식품공학과) ;
  • 정명수 (이화여자대학교 식품공학과)
  • Received : 2014.03.04
  • Published : 2014.08.31

Abstract

The extraction of flavanones such as naringin, narirutin, naringenin, hesperidin, and hesperetin from grapefruit peels was performed using subcritical water extraction (SWE), hot water extraction, and conventional methods such as methanol and ethanol extraction. We analyzed the total flavanone content using high-performance liquid chromatography (HPLC) for each extracting method. Among the three methods, SWE was the optimal method with optimal operating conditions of $170^{\circ}C$ temperature and 10 min operating time. The maximum total flavanone extracted was $86.539{\pm}3.52mg/g$ grapefruit peels. Moreover, we treated the extracts with 60% ${\beta}$-cyclodextrin and then analyzed the surface structure of the encapsulated compounds by field emission-scanning electron microscopy (FE-SEM). The results indicated that the encapsulation in ${\beta}$-cyclodextrin improved solubilization, and the inclusion complex could serve as food supplements.

버려지는 자몽 껍질에 다량 함유되어있는 플라바논을 추출하여 기능성 성분을 재이용하였다. 친환경용매인 아임계수 추출기술을 이용하여 $170^{\circ}C$, 10 min의 최적 조건에서 추출함으로써 무독성 용매로 빠르고 경제적으로 추출할 수 있었다. 자몽껍질 추출물을 ${\beta}$-cyclodextrin을 이용하여 처리함으로써 플라바논과 같은 비극성 물질을 캡슐화하여 소재의 가용화를 용이하게 하였다. 이것은 항산화 기능이 향상된 대체 소재의 개발 및 건강지향식품에 이용할 수 있으며, 이를 통하여 세계 기능성 식품 시장으로의 진출을 위한 발판으로도 삼을 수 있을 것으로 기대된다.

Keywords

References

  1. Peterson JJ, Beecher GR, Bhagwat SA, Dwyer JT, Gebhardt SE, Haytowitz DB, Holden JM. Flavanones in grapefruit, lemons, and limes: A compilation and review of the data from the analytical literature. J. Food Compos. Anal. 19: 74-80 (2006) https://doi.org/10.1016/j.jfca.2005.12.009
  2. Bronner WE, Beecher GR. Extraction and measurement of prominent flavonoids in orange and grapefruit juice concentrates. J. Chromatogr. A. 705: 247-256 (1995) https://doi.org/10.1016/0021-9673(95)00304-6
  3. Justesen U, Knuthsen P. Leth T. Quantitative analysis of flavonols, flavones, and flavanones in fruits, vegetables and beverages by high-performance liquid chromatography with photodiode array and mass spectrometric detection. J. Chromatogr. A. 799: 101-110 (1998) https://doi.org/10.1016/S0021-9673(97)01061-3
  4. Jeon SM, Bok SH, Jang MK, Lee MK, Nam KT, Park YB, Rhee SJ, Choi MS. Antioxidative activity of naringin and lovastatin in high cholesterol-fed rabbits. Life Sci. 69: 2855-2866 (2001) https://doi.org/10.1016/S0024-3205(01)01363-7
  5. Kawaguchi K, Mizuno T, Aida K, Uchino K. Hesperidin as an inhibitor of lipases from procine pancreas and pseudomonas. Biosci. Biotech. Bioch. 61: 102-104 (1997) https://doi.org/10.1271/bbb.61.102
  6. Cha JY, Kim SY, Jeong SJ, Cho YS. Effects of hesperidin and naringenin on lipid concentration in oratic acid treated mice. J. Life Sci. 9: 389-394 (1999)
  7. Shaw PE, Tatum JH, Wilson CW. Improved flavor of navel orange and grapefruit juices by removal of bitter components with ${\beta}$-cyclodextrin polymer. J. Agr. Food Chem. 32: 832-836 (1984) https://doi.org/10.1021/jf00124a034
  8. Wilson CW, Wagner CJ, Shaw PE. Reduction of bitter components in grapefruit and navel orange juices with ${\beta}$-cyclodextrin polymers or XAD resins in a fluidized bed process. J. Agr. Food Chem. 37: 14-18 (1989) https://doi.org/10.1021/jf00085a004
  9. Velickovic DT, Nikolova MT, Ivancheva SV, Stojanovic JB, Veljkovic VB. Extraction of flavonoids from garden (Salvia officinalis L.) and glutinous (Salvia glutinosa L.) sage by ultrasonic and classical maceration. J. Serb. Chem. Soc. 72: 73-80 (2007) https://doi.org/10.2298/JSC0701073V
  10. Yu J, Dandekar DV, Toledo RT, Singh RK, Patil BS. Supercritical fluid extraction of limonoids and naringin from grapefruit (Citrus paradisi Macf.) seeds. Food Chem. 105: 1026-1031 (2007) https://doi.org/10.1016/j.foodchem.2007.04.062
  11. Ko MJ, Cheigh CI, Cho SW, Chung MS. Subcritical water extraction of flavonol quercetin from onion skin. J. Food Eng. 102: 327-333 (2011) https://doi.org/10.1016/j.jfoodeng.2010.09.008
  12. Kalogeropoulos N, Yannakopoulou K, Gioxari A, Chiou A, Makris DP. Polyphenol characterization and encapsulation in ${\beta}$-cyclodextrin of a flavonoid-rich Hypericum perforatum (St John's wort) extract. LWT-Food Sci. Technol. 43: 882-889 (2010) https://doi.org/10.1016/j.lwt.2010.01.016
  13. Sansone F, Picerno P, Mencherini T, Villecco F, D'Ursi AM, Aquino RP, Lauro MR. Flavonoid microparticles by spray-drying: Influence of enhancers of the dissolution rate on properties and stability. J. Food Eng. 103: 188-196 (2011) https://doi.org/10.1016/j.jfoodeng.2010.10.015
  14. Szente L, Szejtli J. Cyclodextrins as food ingredients. Trends Food Sci Tech. 15: 137-142 (2004) https://doi.org/10.1016/j.tifs.2003.09.019
  15. Fang Z, Bhandari B. Encapsulation of polyphenols - a review. Trends Food Sci Tech. 21: 510-523 (2010) https://doi.org/10.1016/j.tifs.2010.08.003
  16. Cheigh CI, Chung EY, Chung MS. Enhanced extraction of flavanones hesperidin and narirutin from Citrus unshiu peel using subcritical water. J. Food Eng. 110: 472-477 (2012) https://doi.org/10.1016/j.jfoodeng.2011.12.019
  17. Ko MJ, Cheigh CI, Chung MS. Relationship analysis between flavonoids structure and subcritical water extraction (SWE). Food Chem. 143: 147-155 (2014). https://doi.org/10.1016/j.foodchem.2013.07.104
  18. Song LX, Bai L, Xu XM, He J, Pan SZ. Inclusion complexation, encapsulation interaction and inclusion number in cyclodextrin chemistry. Coordin Chem Rev. 253: 1276-1284 (2009) https://doi.org/10.1016/j.ccr.2008.08.011