Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Bacterial strains isolated from Jeotgal (salted seafood) induce maturation and cytokine production in mouse bone marrow-derived dendritic cells

마우스 골수 유래 수지상세포의 성숙과 사이토카인 생산에 대한 젓갈 분리균의 효과 연구

  • Moon, Sun-Young (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University) ;
  • Park, Eun-Jin (Department of Food Bioengineering, College of Engineering, Jeju National University) ;
  • Joo, Hong-Gu (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
  • 문선영 (제주대학교 수의과대학 수의약리학실) ;
  • 박은진 (제주대학교 공과대학 식품생명공학과) ;
  • 주홍구 (제주대학교 수의과대학 수의약리학실)
  • Received : 2014.01.20
  • Accepted : 2014.05.08
  • Published : 2014.09.30
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Abstract

Jeotgal (salted seafood) has been one of major fermented foods in Korea for long time. Although there are many studies about Jeotgal in various aspects of food, its immunological importance on hosts has not been elucidated yet. In this study, we investigated if several bacteria isolated from Jeotgal may modulate the function of dendritic cells (DCs), powerful antigen-presenting cells equipped with special immunological capabilities. 4 Jeotgal bacteria were selected as representatives and used for experiments. To treat viable DCs, those bacteria were killed at $60^{\circ}C$ for 30 min. The viability of DCs treated with Jeotgal bacteria was verified and two isolates significantly induced high production of interleukin-12, a representative cell-mediated cytokine of DCs. Surface activation and maturation markers (MHC class II, CD40, CD86) of DCs were analyzed by flow cytometer. In addition, the treated DCs showed significantly high lymphocyte stimulatory capability compared to control DCs based on allogeneic mixed lymphocyte reactions. These observations suggest that Jeotgal isolates can function as immunostimulating bacteria in hosts, like Lactobacillus. Taken together, these experimental evidences may broaden the use of Jeotgal isolates in immunological fields in addition to as a fermented food.

Keywords

References

  1. Adams S, O’Neill DW, Bhardwaj N. Recent advances in dendritic cell biology. J Clin Immunol 2005, 25, 177-188.
  2. Banchereau J, Steinman RM. Dendritic cells and the control of immunity. Nature 1998, 392, 245-252. https://doi.org/10.1038/32588
  3. Bogdan C, Rollinghoff M, Diefenbach A. The role of nitric oxide in innate immunity. Immunol Rev 2000, 173, 17-26. https://doi.org/10.1034/j.1600-065X.2000.917307.x
  4. Byon YY, Kim MH, Yoo ES, Hwang KK, Jee Y, Shin T, Joo HG. Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity, J Vet Sci 2008, 9, 359-365. https://doi.org/10.4142/jvs.2008.9.4.359
  5. Corinti S, Albanesi C, la Sala A, Pastore S, Girolomoni G. Regulatory activity of autocrine IL-10 on dendritic cell functions. J Immunol 2001, 166, 4312-4318. https://doi.org/10.4049/jimmunol.166.7.4312
  6. Guan L, Cho KH, Lee JH. Analysis of the cultivable bacterial community in jeotgal, a Korean salted and fermented seafood, and identification of its dominant bacteria. Food Microbiol 2011, 28, 101-113.
  7. Heufler C, Koch F, Stanzl U, Topar G, Wysocka M, Trinchieri G, Enk A, Steinman RM, Romani N, Schuler G. Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-${\gamma}$ production by T helper 1 cells. Eur J Immunol 1996, 26, 659-668. https://doi.org/10.1002/eji.1830260323
  8. Ishikawa M, Nakajima K, Yanagi M, Yamamoto Y, Yamasato K. Marinilactibacillus psychrotolerans gen. nov., sp. nov., a halophilic and alkaliphilic marine lactic acid bacterium isolated from marine organisms in temperate and subtropical areas of Japan. Int J Syst Evol Microbiol 2003, 53, 711-720. https://doi.org/10.1099/ijs.0.02446-0
  9. Joo HG, Goedegebuure PS, Sadanaga N, Nagoshi M, von Bernstorff W, Eberlein TJ. Expression and function of galectin-3, a ${\beta}$-galactoside-binding protein in activated T lymphocytes. J Leukoc Biol 2001, 69, 555-564.
  10. Joo HG. Altered maturation of dendritic cells by taxol, an anticancer drug. J Vet Sci 2003, 4, 229-234.
  11. Kim HJ, Kim MH, Byon YY, Park JW, Jee Y, Joo HG. Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells. J Vet Sci 2007, 8, 39-44. https://doi.org/10.4142/jvs.2007.8.1.39
  12. Kim MS, Roh SW, Nam YD, Chang HW, Kim KH, Jung MJ, Choi JH, Park EJ, Bae JW. Alishewanella jeotgali sp. nov., isolated from traditional fermented food, and emended description of the genus Alishewanella. Int J Syst Evol Microbiol 2009, 59, 2313-2316. https://doi.org/10.1099/ijs.0.007260-0
  13. Kim MS, Roh SW, Nam YD, Yoon JH, Bae JW. Carnobacterium jeotgali sp. nov., isolated from a Korean traditional fermented food. Int J Syst Evol Microbiol 2009, 59, 3168-3171. https://doi.org/10.1099/ijs.0.010116-0
  14. Kim MS, Roh SW, Bae JW. Halomonas jeotgali sp. nov., a new moderate halophilic bacterium isolated from a traditional fermented seafood. J Microbiol 2010, 48, 404-410. https://doi.org/10.1007/s12275-010-0032-y
  15. Kim SY, Shin S, Koo HC, Youn JH, Paik HD, Park YH. In vitro antimicrobial effect and in vivo preventive and therapeutic effects of partially purified lantibiotic lacticin NK34 against infection by Staphylococcus species isolated from bovine mastitis. J Dairy Sci 2010, 93, 3610-3615. https://doi.org/10.3168/jds.2010-3129
  16. Larsen CP, Ritchie SC, Hendrix R, Linsley PS, Hathcock KS, Hodes RJ, Lowry RP, Pearson TC. Regulation of immunostimulatory function and costimulatory molecule (B7-1 and B7-2) expression on murine dendritic cells. J Immunol 1994, 152, 5208-5219.
  17. Masuda S, Yamaguchi H, Kurokawa T, Shirakami T, Tsuji RF, Nishimura I. Immunomodulatory effect of halophilic lactic acid bacterium Tetragenococcus halophilus Th221 from soy sauce moromi grown in high-salt medium. Int J Food Microbiol 2008, 121, 245-252. https://doi.org/10.1016/j.ijfoodmicro.2007.10.011
  18. Mosca PJ, Hobeika AC, Clay TM, Nair SK, Thomas EK, Morse MA, Lyerly HK. A subset of human monocytederived dendritic cells expresses high levels of interleukin-12 in response to combined CD40 ligand and interferon-ã treatment. Blood 2000, 96, 3499-3504.
  19. Roh SW, Kim KH, Nam YD, Chang HW, Park EJ, Bae JW. Investigation of archaeal and bacterial diversity in fermented seafood using barcoded pyrosequencing. ISME J. 2010, 4, 1-16. https://doi.org/10.1038/ismej.2009.83
  20. Steinman RM. Dendritic cells and the control of immunity: enhancing the efficiency of antigen presentation. Mt Sinai J Med 2001, 68, 160-166.