한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제37권3호
  • /
  • Pages.151-156
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  • 2014
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
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다양한 PRRSV 감염상태에 있는 돼지 혈청을 이용한 PRRS 항체 ELISA 키트들의 비교 평가

Comparative evaluation of two commercial ELISA kits for detection of PRRS antibodies using sera collected from pigs in various stages of PRRSV infection

  • 투고 : 2014.09.13
  • 심사 : 2014.09.26
  • 발행 : 2014.09.30
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초록

Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses to the Korean pig industry. ELISA tests using recombinant nucleocapsid protein of PRRSV have been most commonly used for PRRS diagnostics. In the current study, two commercial PRRSV ELISA kits (Bionote PRRSV Antibody ELISA and IDEXX 3XR PRRS Antibody ELISA) have been compared using sera collected from 19 swine farms in various stages of PRRSV infection confirmed by professional diagnostic centers. Thus 130 sera collected from 5 different farms with active PRRSV infection, 130 sera from 6 different farms with PRRS-stabilized status, and 140 sera from 8 different farms with PRRS-free status were evaluated to determine the correlation of test results between those ELISA kits. Both ELISA kits showed a good correlation [PRRSV-positive farms ($R^2$=0.6375) and stabilized farms ($R^2$=0.8928)] in sample-to-positive (S/P) ratio va lues. Among the 140 sera from negative farms, one sample was falsely positive by either of the ELISA kits. In conclusion, both of the ELISA kits showed a good correlation when applied on field samples collected from farms at various stages of PRRSV infection. Bionote ELISA or IDEXX ELISA gave a false positive result on 1 out of 140 negative samples so their specificity was calculated as 99.3%. Therefore, Bionote ELISA would be a good complementary and alternative method for IDEXX ELISA kit, and vice versa.

키워드

참고문헌

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피인용 문헌

  1. Seroprevalence of porcine reproductive and respiratory syndrome (PRRS) in Gyeongbuk province vol.38, pp.1, 2015, https://doi.org/10.7853/kjvs.2015.38.1.9
  2. Seroprevalence of porcine reproductive and respiratory syndrome (PRRS) and porcine circovirus-2 (PCV-2) in pig farms in Gyeongbuk province vol.38, pp.3, 2015, https://doi.org/10.7853/kjvs.2015.38.3.163