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Opuntia humifusa (Raf.) Raf. f. jeollaensis E. J. Kim & S. S. Whang, a new forma based on three DNA markers

DNA 염기서열에 근거한 선인장과 신품종 왕가시천년초[Opuntia humifusa (Raf.) Raf. f. jeollaensis E. J. Kim & S. S. Whang]

  • Kim, Eun Jeong (Division of Science Education, Chonbuk National University) ;
  • Srikanth, Krishnamoorthy (Division of Science Education, Chonbuk National University) ;
  • Lee, Eunae (Division of Science Education, Chonbuk National University) ;
  • Whang, Sung Soo (Division of Science Education, Chonbuk National University)
  • Received : 2014.07.31
  • Accepted : 2014.09.15
  • Published : 2014.09.26

Abstract

The taxonomic status of a new forma, Opuntia humifusa (Raf.) Raf. f. jeollaensis E. J. Kim & S. S. Whang, and the taxonomic relationships of three Korean cladodes were studied based on DNA sequences of three genes. The new forma O. humifusa f. jeollaensis (Wanggasi-Chunnyuncho) is simlilar to O. humifusa (Chunnyuncho), but differ in having both flower with the reddish inner center, and strong and many 3 cm long spines. Molecular studies showed that the new forma grouped into Macrocentra series instead of Humifusa series which included Korean O. humifusa. We designated the new taxa firstly studied here as a new forma, because the taxa have been mainly cultivated in farmlands in Jeolla-do area rather than distributed in nature. The taxonomic relationships of three Korean cladodes are discussed in detail.

세 개 유전자의 DNA 분자서열을 이용해서 전라도 지역에서 재배되고 있는 신품종 왕가시천년초(O. humifusa (Raf.) Raf. f. jeollaensis E. J. Kim & S. S. Whang)의 분류학적 지위와 한국산 손바닥 선인장들의 분류학적 유연관계가 조사되었다. 신품종 왕가시천년초는 형태학적으로나 생육 지역이 천년초와 비슷하지만, 노란색 꽃의 안쪽 중심에 붉은 색이 있고, 3 cm 길이의 강모가 많이 발달되어서 차이가 있다. 분자적인 연구결과는 왕가시천년초가 O. humifusa complex에서 Macrocentra 계열에 포함되어 Humifusa 계열에 포함되는 한국산 O. humifusa와 다르게 나타났다. 왕가시천년초는 천년초와 마찬가지로 주로 농장에서 재배되고 있어서 품종으로 지위를 주었다. 한국산 손바닥선인장 3종에 대한 분류학적 유연관계가 자세히 논의되었다.

Keywords

References

  1. Anderson, E. F. 2001. The cactus family. Porland, Oregon, USA, Timber Press.
  2. Barcenas, R. T., T. Yasson, and J. A. Hawkins. 2011. Molecular systematics of the Cataceae. Cladistics 27: 1-20. https://doi.org/10.1111/j.1096-0031.2010.00316.x
  3. Benson, L. 1982. The cacti of the United States and Canada. Stanford University Press.
  4. Benson, L. and D. L. Walkington. 1965. The southern Californian prickly pears: invasion, adulteration, and trial-by-fire. Annals of Molecular Botanical Garden 52: 252-273.
  5. Britton, N. L. and J. N. Rose. 1919. The Cactaceae. Carnegi Institute of Washington, Washington DC, USA
  6. Casas, A. and G. Barbera. 2002. Mesoamerican domestication and diffusion. In Carti: Biology and Uses. Nobel, P. S. (ed.), University of California Press, Berkeley, CA. Pp. 143-162.
  7. Cho, E. J., M. J. Kim and W. S. Choi. 2006. Quality properties of Jeung-pyun with added withprickly pear (Cheonnyuncho) Powder. Journal of East Asian Society of Dietary Life 17: 903-910. (in Korean)
  8. Choi, S. H. 2014. Antioxidant effects and melanin synthesis inhibition from Opuntia humifusa extract. Master thesis, Hoseo University, Korea,
  9. Choi, J. H. and K. S. Shin. 2011. Structural analysis of anti-metastatic polysaccharides isolated from Opuntia humifusa. Journal of Korean Society of Food Science Nutrition 40: 214-222. https://doi.org/10.3746/jkfn.2011.40.2.214
  10. Choi, H. J., S. C. Park and T. H. Hong. 2005. Anti-tumor activity of fermented liquid Opunita humifusa in cervical cancer cells and its chemical composition. Journal of Korean Society of Food Science Nutrition 34: 1525-1530. (in Korean) https://doi.org/10.3746/jkfn.2005.34.10.1525
  11. Crozier, B. S. 2004. Subfamilies of Cactaceae Juss., including Blossfeldioideae subfam. nov. Phytologia 86: 52-65.
  12. Doyle, J. J. and J. L. Dolyle. Isolation of plant DNA from fresh tissue. Focus 12: 13-15
  13. Edwards, E. J., R. Nyffeler and M. J. Donoghue. 2005. Basal cactus phylogeny: implications of Pereskia (Cactaceae) paraphyly for the transition to the cactus life form. The American Naturalist 92: 1177-1188.
  14. Griffith, M. P. 2004. The origins of an important cactus crop. Opuntia ficus-indica (Cactaceae): New molecular evidence. Americal Journal of Botany 91: 1915-1921. https://doi.org/10.3732/ajb.91.11.1915
  15. Griffith, M. P. and M. Porter. 2009. Phylogeny of Opuntioideae (Cactaceae). International Journal of Plant Sciences 170: 107-116. https://doi.org/10.1086/593048
  16. Hall, T. A. 1999. Bioedit: a user-friendly biological sequence alignment editor and analysis program for Window 95/98/ NT. Nucleic Acids Symposium Series 41: 95-98.
  17. Hernndez-Hernndez, J., H. Hernndez, J. A. de-Nova, R. Puente, L. E. Eguiarte, and S. Magalln. 2011. Phylogenetic relationships and evolution of growth form in Cactaceae (Caryophyllales, Eudicotyledoneae). American Journal of Botany 98: 44-61. https://doi.org/10.3732/ajb.1000129
  18. Huang, X., Q. Li, Y. Zhang, Q. Lu, L. Guo, L. Huang and Z. He. 2008. Neuroprotective effects of cactus polysaccharide on exygen and glucose deprivation induced damage in rat brain slies. Cell and Molecular Neurobiology 28: 559-568. https://doi.org/10.1007/s10571-007-9184-7
  19. Inglese, P., F. Basile and M. Schirra. 2002. Cactus pear fruit production. In Cacti: Biology and Uses. Nobel, P. S. (ed.), University of California Press, Berkeley, CA. Pp. 163-183.
  20. Jun, H. I., M. N. Cha, E. I. Yang, D. G. Choi and Y. S. Kim. 2013. Physicochemical properties and antioxidant activity of Korean cactus (Opuntia humifusa) cladodes. Horticulture, Environmental Biotechnology 54: 288-295. https://doi.org/10.1007/s13580-013-0145-4
  21. Jung, B. M., M. O. Shin, and H. R. Kim. 2012. The effects of animicrobial, antioxidant, and anticancer properties of Opuntia humifusa stems. Journal of Korean Society of Food Science Nutrition 41: 20-25. https://doi.org/10.3746/jkfn.2012.41.1.020
  22. Kim, J. H. 2013. Increase of extraction efficiency and radical scavenging activity of cactus cladodes by biological treatment and its application. Ph.D dissertation, Korea University, Korea.
  23. Kwon, D. K. and Y. J. Song. 2005. Effect of Opuntia humifusa supplementation on endurance exercise performance in rats fed a high-fat diet. The Korean journal of Exercise Nutrition 9: 183-188. (in Korean)
  24. Laurenz, J. C., C. C. Collier and J. O. Kuti. 2003. Hypoglycaemic effect of Opuntia l indheimeri Engelm. in a diabetic pig model. Phytotherapy Research 17: 26-29. https://doi.org/10.1002/ptr.1052
  25. Lee, K. S., M. G. Kim and K. Y. Lee. 2004. Antimicrobial effect of the extracts of cactus Chounnyouncho (Opuntia humifusa) against food borne pathogens. Journal of Korean Society of Food Science Nutrition 29: 847-853. (in Korean)
  26. Leuenberger, B. E. 2001. The South American Opuntia ser. Armatae (= O. ser. Elatae) (Cactaceae). Botanische Jahrbucherfur Systematick, Pfanzengeschichte und Pflanzengeographie 123: 413-439.
  27. Majdoub, H., L. Piction, D. Cerf and S. Roudesli. 2010. Water retention capacity of polysaccharides from prickly pear nopals of Opuntia ficus indica and Opuntia Litoralis: Physical-chemical approach. Journal of Polysaccarides and Environment 18: 451-458. https://doi.org/10.1007/s10924-010-0159-2
  28. Majure, L. C., R. Puente, M. P. Griffith, W. S. Judd, P. S. Soltis and D. E. Soltis. 2012a. Phylogeny of Opuntia s.s. (Cactaceae): reticulate evolution, geographic origins, and clade delineation. American Journal of Botany 99: 847-864. https://doi.org/10.3732/ajb.1100375
  29. Majure, L. C., D. E. Soltis, P. S. Soltis, and W. S. Judd. 2012b. Cytogeography of the Humifusa clade of Opuntia s.s. (Cataceae: Opuntioideae): Correlations with geographic distributions and morphological differentiation of a polyploid complex. Comparative Cytogenetics 6: 53-77. https://doi.org/10.3897/compcytogen.v6i1.2523
  30. Mauseth, J. D. 2006. Structure-function relationships in highly modified shoots of Cactaceae. Annals of Botany 98: 901-926. https://doi.org/10.1093/aob/mcl133
  31. Ncibi, S., M. B. Othman, A. Akacha, M. N. Krifi and L. Zourgui. 2008. Opuntia ficus-indica extract protects against chlorphrifos- induced damage on mice liver. Food and Chemical Toxicology 46: 797-802. https://doi.org/10.1016/j.fct.2007.08.047
  32. Nefzaoui, A. and H. B. Salem. 2002. Forage, fodder, and animal nutrition. In Cacti: Biology and Uses. Nobel, P. S. (ed.) University of California Press, Berkeley, CA. Pp. 199-210.
  33. Nyffeler, R. and U. Eggli. 2010. A farewell to dated ideas and con cepts: molecular phylogenetics and a revised suprageneric classification of the family Cactaceae. Schumannia 6: 109-149.
  34. Parfitt, B. D. and D. J. Pinkava. 1988. Nomenclatural and Systematic reassessment of Opuntia engelmannii and O. lindheimeri (Cactaceae) Modrono 35: 342-349.
  35. Reyes-Aguero, J. A., F. Carlin-Castelan, J. R. Aguirre, H. M. Heranndez. 2007. Preparation of Opuntia herbarium speciemens. Haseltonia 13: 76-82. https://doi.org/10.2985/1070-0048(2007)13[76:POOHS]2.0.CO;2
  36. Stintzing, F. C. and R. Carle. 2005. Cactus stems (Opuntia spp.): A review on their chemistry, technology, and uses. Molecular Nutrition and Food Research 49: 175-194. https://doi.org/10.1002/mnfr.200400071
  37. Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28: 2731-2739. https://doi.org/10.1093/molbev/msr121
  38. Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin and D. G. Higgins. 1997. The CLUSTAL-X windows interface: Flexible strategies for multiple sequence alignment adied by quality analysis tools. Nucleic Acids Research 25: 4876-4882. https://doi.org/10.1093/nar/25.24.4876
  39. Wallace, R. S. and S. L. Dickie, 2002. Systematic implications of chloroplast DNA sequences variation in the Opuntioideae. In Studies in the Opuntioideae (Cactaceae). Hunt Taylor, H. and D. Hunt, (eds.), The manse, Chapel Lane, Milborne Port Sherborne, England. Pp. 9-24.
  40. Yang, E. I. 2013. Physicochemical properties and biological activities of alcohol-insoluble polysaccharide from Opuntia humifusa. Ph.D dissertation, Chonbuk National University, Korea.