The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Pseudomonas azotoformans HC5 Effective in Antagonistic of Mushrooms Brown Blotch Disease Caused by Pseudomonas tolaasii

버섯 세균갈색무늬병균(Pseudomonas tolaasii)에 항균활성을 가지는 미생물 Pseudomonas azotoformans HC5

  • 이찬중 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영미 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 한주연 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 전창성 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 정종천 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 문지원 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 서장선 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 한혜수 (흙살림) ;
  • 차재순 (충북대학교 식물의학과)
  • Received : 2014.05.07
  • Accepted : 2014.06.23
  • Published : 2014.09.30
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Abstract

A gram-negative bacterium was isolated from spent substrate of Agaricus bisporus and showed marked antagonistic activity against Pseudomonas tolaasii. The bacterium was identified as Pseudomonas azotoformans by based on the cultural, biochemical and physiological characteristics, and 16S rRNA gene sequence. The isolated bacterium was saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell was not sufficient for inhibition in vitro. Control efficacy of Pseudomonas azotoformans HC5 to brown blotch of P. tolaasii was 73, 78, and 71% on A. bisporus, Flammulina velutipes, and Pleurotus ostreatus, respectively. In the future, the suppressive bacterium may be useful for development of a biocontrol system.

P. tolaasii에 의해 발생하는 세균갈색무늬병은 버섯재배에서 문제가 되는 대표적인 병해이다. 본 연구에서는 세균갈색무늬병의 생물학적 방제법에 이용할 수 있는 길항미생물의 항균활성과 선발된 길항미생물에 대해 폿트 수준의 생물검정 실험을 실시하였다. 재배중인 느타리 폐면배지와 양송이 퇴비에서 세균갈색무늬병원균을 강하게 억제하는 길항세균 HC5를 선발하였으며, 생리 생화학적 실험과 유전적 실험결과 HC5균주는 P. azotoformans로 동정되었다. P. azotoformans HC5를 양송이, 팽이버섯, 느타리에 처리한 결과 각각 78%, 73%, 71%의 방제효과를 보였다. 따라서 P. azotoformans HC5가 버섯 세균갈색무늬병 방제를 위해 합성농약을 대체할 수 있는 친환경 방제제가 될 수 있을 것으로 생각된다.

Keywords

References

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