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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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XS-E is Induced Atopic Dermatitis NC/Nga Mice the Impact of Skin Conditions

XS-E가 아토피피부염이 유발된 NC/Nga Mice의 피부상태에 미치는 영향

  • Kim, Kum-Lan (Department of Beauty and Art, Suwon Women's University)
  • 김금란 (수원여자대학교 미용예술학과)
  • Received : 2014.07.17
  • Accepted : 2014.08.11
  • Published : 2014.08.31
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Abstract

This study reports significant improvement of atopic dermatitis condition as a result of experiment using Xanthium strumarium L. extract (XS-E) at the dorsal skin of induced atopic dermatitis Nc/Nga mice. Skin clinical score has decreased ($2.75{\pm}0.85$, *p<0.05), showing visible change of skin condition. IgE (***p<0.001) and IgG1 ($2522.00{\pm}32.80$, ***p<0.001) in plasma also decreased significantly. mRNA (gene expression) level increased ($RQ=2.75{\pm}0.10$, ***p<0.001) within skin tissue of CD4+CD25+Foxp3+ Treg cell that's activated by XS-E dosage, thereby discovering that there is an effect of suppressing proliferation and viability of Th2 cell, eosinophils, mast cell and inflammatory cell. Upon examining cells permeated with H&E and toluidine blue staining technique, thickness of epidermis and mast cell's permeation decreased, and the result of examining the distribution of CCR 3+ eosinophils within ALN showed that it's level fell down to that of wild type (normal group, NC/Nga-WT). By such results, it is suggested that XS-E is highly effective on atopic dermatitis, and it is considered that continued quantitative research and case study of clinical research such as effect of cell number in individual tissues or change of total cell number are necessary.

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References

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