KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Analysis of Morphological Change of Polar Bacterium using Microfluidic Device with Temperature Gradient

온도 구배가 있는 미세유체 장치를 이용한 극지 미생물의 형태 변화 분석

  • Jeong, Seong-Geun (Department of Chemical Engineering, Chungnam National University) ;
  • Park, Aeri (Department of Chemical Engineering, Chungnam National University) ;
  • Jeong, Heon-Ho (Department of Chemical Engineering, Chungnam National University) ;
  • Hong, Soon Gyu (Division of Polar Life Sciences, Korea Polar Research Institute) ;
  • Lee, Chang-Soo (Department of Chemical Engineering, Chungnam National University)
  • 정성근 (충남대학교 공과대학 화학공학과) ;
  • 박애리 (충남대학교 공과대학 화학공학과) ;
  • 정헌호 (충남대학교 공과대학 화학공학과) ;
  • 홍순규 (극지연구소, 극지생명과학연구부) ;
  • 이창수 (충남대학교 공과대학 화학공학과)
  • Received : 2014.07.11
  • Accepted : 2014.08.25
  • Published : 2014.08.31
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Abstract

We present microfluidic method to rapidly analyze the effect of temperature on the change of morphologies of Antarctic bacteria (Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp.). The microfluidic device is able to generate stable temperature gradient from 7 to$40^{\circ}C$ and dramatically reduce the number of experiments, experimental cost and labor, and amount of sample. Based on this approach, we found that specific bacteria transforming morphology into filament or elongated body strongly depends on cultivation temperature. Interestingly, we found that the morphologies of Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp. are elongated at below $25^{\circ}C$, above $20^{\circ}C$, above $15^{\circ}C$ and above $35^{\circ}C$, respectively. We envision the microfluidic device is a useful approach to analyze biological events with a high throughput manner.

Keywords

References

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