대한본초학회지 (The Korea Journal of Herbology)

  • 제29권5호
  • /
  • Pages.45-53
  • /
  • 2014
  • /
  • 1229-1765(pISSN)
  • /
  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

DOI QR Code

비피(鼻皮) 증류액의 항균, 소염, 항산화 작용에 대한 연구

Studies on the Anti-bacterial, Anti-inflammatory and Anti-oxidant Effect of BPH

  • 김호선 (가천대학교 한의과대학 본초학교실) ;
  • 이영종 (가천대학교 한의과대학 본초학교실)
  • Kim, Ho-Seon (Dept. of Herbology, College of Korean Medicine, Gachon University) ;
  • Lee, Young-Jong (Dept. of Herbology, College of Korean Medicine, Gachon University)
  • 투고 : 2014.08.05
  • 심사 : 2014.09.18
  • 발행 : 2014.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : The purpose of the study is to test the antibacterial, anti-inflammatory and antioxidant effects of BPH, which is composed of Pini Densiflorae Nodi Lignum and Querci Acutissimae Fructus, Angelicae Gigantis Radix, Cnidii Rhizoma, Angelicae Dahuricae Radix, Angelicae Tenuissimae Radix. Method : Antibacterial and anti-inflammatory effects of BPH on Propionibacterium acnes, one of anaerobic bacteria species were evaluated by measuring the levels of 2,2-diphenyl-1-picrylhydrazyl (DPPH) elimination and lipid peroxidation. Result : When BPH was applied to CCD-986sk (Human normal fibroblast) to confirm the level of cytokine(tumor necrosis factor-alpha, interleukin-8), its level increased in proportion to that of BPH's concentration, which indicated dose-dependent relationship. Using the Disk diffusion to measure the bacterial growth inhibition zone varying BPH concentration, it was found that the antibacterial effect of BPH was less than that of erythromycin, the control group, but was higher than that of saline, and it increased with higher concentrations. In a liquid culture medium containing BPH, the growth rate of Propionibacterium acnes was decreased by more than 10% at 25% BPH. After adding P. acnes to THP-1 monocyte, and treated it with BPH, and measuring the concentration of TNF-a and IL-8, it was observed that the amount of TNF-alpha and IL-8 significantly decreased depending on the level of BPH concentration. The ability to eliminate DPPH increased with higher BPH concentration. The inhibition of lipid peroxidation was increased by BHT treatment in a dose-dependent manner. Conclusion : Using Propionibacterium acnes, an anaerobic bacteria, we confirmed that BPH has antibacterial, anti-inflammatory and antioxidant effects.

키워드

참고문헌

  1. Tao HJ. Mingyibielu. Beijing : Renminweishengchubanshe. 1986 : 18.
  2. State Administration of Traditional chiense medicine of the People's Republic of China. Zhonghuabencao. Vol. 4. Shanghai : Shanghai Scientific and Technical Publishers. 1999 : (2) 293-308, 431-6, (5) 883-8, 976-83, 986-990.
  3. Shang ZJ. Xinxiubencao. Anhui : Kexuejishuchubanshe. 1981 : 301-2, 360.
  4. Jiangsuxinyixueyuan. Zhongyaodacidian. Shanghai : Shanghaikexuejishuchubanshe. 1979 : 1252, 2591-2.
  5. Zhongguoyixuekexueyuan. Zhongyaozhi. Beijing : Renminweishengchubanshe. 1994 : 626-32.
  6. Nanjingyaoxueyuan. Yaocaixue. Beijing : Renminweishengchubanshe. 1960 : 307.
  7. Quanguozhongcaoyaohuibianbianxiezu. Quanguozhongcaoyaohuibian. Beijing : Renminweishengchubanshe. 1983 : 625-6.
  8. Evance CR, Halliwell B, Lunt GG. Free radicals and oxidative stress : environment, drugs and food additives. London : Portland Press. 1995 : 1-31.
  9. Sozmen EY, Tanyalcin T, Onat T, Kutay F, Erlacin S. Ethanol induced oxidative stress and membrane injury in rat erythrocytes. Eur J Clin Chem Clin Biochem. 1994 ; 32(10) : 741-4.
  10. Chen GT. Bencaogangmutongshi. Beijing : xueyuanchubanshe. 1992 : 1567-71.
  11. Lee WC. Hankuksikmulmyungko. Seoul : Academyseojeok. 1996 : 120-1.
  12. Wu Y. Rihuazibencao. Hefei : Anhuikexuejishuchubanshe. 2005 : 146.
  13. PMSLite. Phytonutrient Profile of Tribulus (Tribulus Terrestris), India Herbs - Ancient Remedies For Modern Times, Available from: http://www.pmslite.com/plant_phytochemistry.htm ?plantid=2053. Accessed 27 Dec. 2008.
  14. Korea Food & Drug Administration. The Korean Pharmacopoeia. 10th Edition. Chungwon : KFDA. 2014 : 1122, 1142, 1203.
  15. Kim IR, Kim HC, Kuk YB, Park SJ, Park YK, Park JH, Seo BI, Seo YB, Shin MK, Lee YJ, LeeYC, Lee JH, Leem KH, Cho SI, Chung JK, Joo YS, Choi HY. Boncho-Hak. Seoul : Young-Lim Press. 2007 : 629-31.
  16. Korea Food & Drug Administration. The Korean herbal pharmacopoeia 4th. Chungwon : KFDA. 2013 : 31.
  17. Webster GF, Leyden JJ, Norman ME, Nilsson UR. Complement activation in acne vulgaris: in vitro studies with Propionibacterium acnes and Propionibacterium granulosum. Infect Immun. 1978 ; 22(2) : 523-9.
  18. Kim J. Review of the innate immune response in acne vulgaris: activation of Toll-like receptor 2 in acne triggers inflammatory cytokine responses. Dermatology. 2005 ; 211(3) : 193-8. https://doi.org/10.1159/000087011
  19. Downing DT, Stewart ME, Wertz PW, Strauss JS. Essential fatty acids and acne. J Am Acad Dermatol. 1986 ; 14 : 221-5. https://doi.org/10.1016/S0190-9622(86)70025-X
  20. Webster GF, Leyden JJ, Tsai CC, Baehni P, McArthur WP. Polymorphonuclear leukocyte lysosomal release in response to Propionibacterium acnes in vitro and its enhancement by sera from inflammatory acne patients. J Invest Dermatol. 1980 ; 74(6) : 398-401. https://doi.org/10.1111/1523-1747.ep12544494
  21. Im DY. Yakrihak. Seoul : Shinilbukseu. 2006 : 373-4.
  22. Park J, Lee J, Jung E, Park Y, Kim K, Park B, Jung K, Park E, Kim J, Park D. In vitro antibacterial and anti-inflammatory effects of honokiol and magnolol against Propionibacterium sp. Eur J Pharmacol. 2004 ; 496 : 189-95. https://doi.org/10.1016/j.ejphar.2004.05.047
  23. Tracey KJ, Cerami A. Metabolic responses to cachectin/TNF. Ann N Y Acad Sci. 1990 ; 587 : 325-31. https://doi.org/10.1111/j.1749-6632.1990.tb00173.x
  24. Beutler B, Grau GE. Tumor necrosis factor in the pathogenesis of infectious diseases. Crit Care Med. 1993 ; 21 : S423-35. https://doi.org/10.1097/00003246-199310001-00002
  25. Vowels BR, Yang S, Leyden JJ. Induction of proinflammatory cytokines by a soluble factor of Propionibacterium acnes: implications for chronic inflammatory acne. Infect Immun. 1995 ; 63(8) : 3158-65.
  26. Hurtado-Nedelec M, Chollet-Martin S, Nicaise-Roland P, Grootenboer-Mignot S, Ruimy R, Meyer O, Hayem G. Characterization of the immune response in the synovitis, acne, pustulosis, hyperostosis, osteitis (SAPHO) syndrome. Rheumatology (Oxford). 2008 ; 47(8) : 1160-7. https://doi.org/10.1093/rheumatology/ken185
  27. Harman D. The free radical theory of aging : In free radicals in Biolog . Vol 5. New York : Academic Press. 1982 : 255-75.
  28. Feher J, Csomos G, Vereckei A. The free radical theory of aging, Free radicals reactions in medicine. Berlin: Springer-Verlag. 1987 : 57-9.
  29. Heicklen J, Brown E. Increase in life expectancy for mice fed diethylhydroxylamine(DEHA). J Gerontol. 1987 ; 42(6) : 674-80. https://doi.org/10.1093/geronj/42.6.674
  30. Oyanagui Y. SOD and active oxygen modulator. Tokyo : Nihon Jgakukan. 1989 : 17-36.
  31. Kellogg EW 3rd, Fridovich I. Liposome oxidation and erythrocyte lysis by enzymically generated superoxide and hydrogen peroxide. J Biol Chem. 1977 ; 252(19) : 6721-8.
  32. Halliwell B, Gutteridge JCM. Free Radicals in Biology and Medicine. Oxford : Clarendon Press. 1989 : 228-33.