Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Cytotoxicity of Extracts from Korean Pepper (Capsicum annuum L.) by Extraction Solvents and Plant Parts

추출용매와 부위에 따른 고추 추출물의 세포독성

  • Choi, So Ra (Jeollabukdo Agricultural Research & Extension Services) ;
  • Kim, Myung Jun (Institute of Agricultural Science and Technology, Chonbuk National Univ.) ;
  • Ahn, Min Sil (Jeollabukdo Agricultural Research & Extension Services) ;
  • Song, Eun Ju (Jeollabukdo Agricultural Research & Extension Services) ;
  • Seo, Sang Young (Jeollabukdo Agricultural Research & Extension Services) ;
  • Choi, Min Kyung (Jeollabukdo Agricultural Research & Extension Services) ;
  • Kim, Young Sun (Jeollabukdo Agricultural Research & Extension Services) ;
  • Choi, Dong Geun (College of Agriculture and Life Science, Chonbuk National Univ.) ;
  • Song, Young Ju (Jeollabukdo Agricultural Research & Extension Services)
  • Received : 2014.07.07
  • Accepted : 2014.09.01
  • Published : 2014.10.30
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Abstract

In order to find out anticancer activity of Korean pepper (Capsicum annuum L.), the cytotoxicity against 8 cell lines including 293 (normal kidney cells) and A-431 (epidermoid carcinoma cells) of extracts by extraction solvents and plant parts were investigated using MTT assay. Also the correlation between content of capsaicin known as anticancer ingredient and cytotoxicity of extracts from pepper were analyzed. The distilled water extracts from seed and germinated seed showed very high cytotoxicity against 6 cancer cell lines including A549 (lung carcinoma cells), AGS (stomach adenocarcinoma cells), HeLa (cervix adenocarcinoma cells), HepG2 (hepatoblastoma cells), HT-29 (colon adenocarcinoma cells), and MCF-7 (breast adenocarcinoma cells). But 80% ethanol and methanol extracts showed cytotoxicity against 293 and AGS. The $RC_{50}$, that was, the concentration of sample required for 50% reduction of cell viability, of seed and germinated seed extracts against AGS were $33.4{\sim}389.1{\mu}g/m{\ell}$ and $63.9{\sim}1,316.7{\mu}g/m{\ell}$, respectively, so anticancer activity was higher in seed than in germinated seed. In capsaicin contents, seed with high cytotoxicity and pericarp with a little cytotoxicity contained $47.4{\sim}1,260.0{\mu}g/g$ and $58.3{\sim}1,498.0{\mu}g/g$, respectively. As these results, the correlation was not between cytotoxicity and capsaicin content.

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References

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