Journal of Korean society of Dental Hygiene (한국치위생학회지)

Korean Society of Dental Hygiene (한국치위생학회)
권호 표지
DOI QR코드

DOI QR Code

The protective effect of coenzyme Q10 on cytotoxicity of regin monomer of odontoblast caused by TEGDMA

코엔자임 Q10 처리에 따른 TEGDMA에 의해 유발된 치아 세포 사멸 억제 효과

  • Lee, Ahreum (Department of Dental Hygiene, Dongseo University) ;
  • Park, Soyeong (Department of Dental Hygiene, Dongseo University) ;
  • Lee, Kyung Hee (Department of Dental Hygiene, Dongseo University)
  • 이아름 (동서대학교 치위생학과) ;
  • 박소영 (동서대학교 치위생학과) ;
  • 이경희 (동서대학교 치위생학과)
  • Received : 2014.07.12
  • Accepted : 2014.09.25
  • Published : 2014.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : The purpose of the study is to investigate the protective effect of coenzyme $Q_{10}$ on cytotoxicity effect of dental monomers in odontoblast(MDPC-23). Methods : MDPC-23 was incubated with the(co)monomers triethylene glycol dimethacrylate (TEGDMA) with and without addition of coenzyme $Q_{10}$. The cell proliferation and survival was determined using WST-1 assay. The level of reactive oxygen species(ROS) was measured by immunofluorescent staining for DCF-DA. Results : TEGDMA treatment decreased the cell proliferation by dose dependently(0.1, 1, 2.5, 5, 10 mM) on the growth of MDPC-23 cells. Coenzyme $Q_{10}$ showed cell proliferation from 5 to $500{\mu}M$ by WST-1 assay. Pre-treatment coenzyme $Q_{10}$ showed the antioxidant effect on proliferation and viability of MDPC-23 after 48h(p<0.05). The positive cells were observed in non-coenyme $Q_{10}$ treatment group(group 2) in comparison with coenyme $Q_{10}$ pre-treatment group(group 1) by DCF-DA. The fluorescence positive cells showed 14.715(group 1) and 19.788(group 2) using image J system. Conclusions : TEGDMA induced cytotoxicity. The MDPC-23 cell death was associated with the increasing ROS. Coenyme $Q_{10}$ showed the antioxidant effects by decreasing ROS. This effects may contribute to the treatment of periodontal disease induced by TEGDMA after operation.

Keywords

References

  1. Lee YK, Park JR. The relationship of obesity and periodontal disease by age. J Korean Soc Dent Hyg 2013; 13(6): 1015-21. http://dx.doi.org/10.13065/jksdh.2013.13.06.1015.
  2. Choi BBR. Effect of autophagy in human tongue squamous cell carcinoma SCC 25 cells from Scutellariae Radix by ethanol extract. J Korean Soc Dent Hyg 2014; 14(2): 287-92. http://dx.doi.org/10.13065/jksdh.2014.14.02.287.
  3. Ferracane JL. Current trends in dental composites. Crit Rev Oral Biol Med 1995; 6(4): 302-18. http://dx.doi.org/10.1177/10454411950060040301.
  4. Issa Y, Watts DC, Brunton PA, Waters CM, Duxbury AJ. Resin composite monomers alter MTT and LDH activity of human gingival fibroblasts in vitro. Dent Mater 2004; 20(1): 12-20. http://dx.doi.org/10.1016/S0109-5641(03)00053-8.
  5. Bakopoulou A, Papadopoulo T, Garefis P. Molecular toxicology of substances released from resin-based dental restorative materials. Int J Mol Sci 2009; 10(9): 3961-99. http://dx.doi.org/10.3390/ijms10093861.
  6. Engelmann J, Leyhausen G, Leibfritz D, Geurtsen W. Metabolic effects of dental resin components in vitro detected by NMR spectroscopy. J Dent Res 2001; 80(3): 869-75. http://dx.doi.org/10.1177/00220345010800030501.
  7. Stanislawski L, Lefeuvre M, Bourd K, Soheili-Majd E, Goldberg M, Perianin A. TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species. J Biomed Mater Res A 2003; 66(3): 476-82. http://dx.doi.org/10.1002/jbm.a.10600.
  8. Jang JH, Kim YI, Lee H. Antimicrobial activity of Prunus mume extract to oral microbes. J Korean Soc Dent Hyg 2014; 14(1): 109-15. http://dx.doi.org/10.13065/jksdh.2014.14.01.109.
  9. Schweikl H, Schmalz G, Rackebrandt K. The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V79 cells. Mutat Res 1998; 415(1-2): 119-30. http://dx.doi.org/10.1016/S1383-5718(98)00067-9.
  10. Kleinsasser NH, Wallner BC, Harreus UA, Kleinjung T, Folwaczny M, Hickel R, et al. Genotoxicity and cytotoxicity of dental materials in human lymphocytes as assessed by the single cell microgel electrophoresis (comet) assay. J Dent 2004; 32(3): 229-34. http://dx.doi.org/10.1016/j.jdent.2003.11.002.
  11. Lefeuvre M, Amjaad W, Goldberg M, Stanislawski L. TEGDMA induces mitochondrial damage and oxidative stress in human gingival fibroblasts. Biomaterials 2005; 26: 5130-7. http://dx.doi.org/10.1016/j.biomaterials.2005.01.014.
  12. Rajendran P, Nandakumar N, Rengarajan T, Palaniswami R, Gnanadhas EN, Lakshminarasaiah U, et al. Antioxidants and human diseases. Clin Chim Acta 2014; 436(25): 332-47. http://dx.doi.org/10.1016/j.cca.2014.06.004.
  13. Chen SJ, Allam JP, Duan YG, Haidl G. Influence of reactive oxygen species on human sperm functions and fertilizing capacity including therapeutical approaches. Arch Gynecol Obstet 2013; 288(1): 191-9. http://dx.doi.org/10.1007/s00404-013-2801-4.
  14. Wajda R, Zirkel J, Schaffer T. Increase of bioavailability of coenzyme Q(10) and vitamin E. J Med Food 2007; 10: 731-4. http://dx.doi.org/10.1089/jmf.2006.254.
  15. Young AJ, Johnson S, Steffens DC, Doraiswamy PM. Coenzyme Q10: a review of its promise as a neuroprotectant. CNS Spectr 2007; 12(1): 62-8.
  16. Engel RH1, Evens AM. Oxidative stress and apoptosis: a new treatment paradigm in cancer. Front Biosci 2006; 11: 300-12. https://doi.org/10.2741/1798
  17. Brock GR, Butterworth CJ, Matthews JB, Chapple IL. Local and systemic total antioxidant capacity in periodontitis and health. J Clin Periodontol 2004; 31(7): 515-21. http://dx.doi.org/10.1111/j.1600-051X.2004.00509.
  18. Kuzmanova D, Jansen ID, Schoenmaker T, Nazmi K, Teeuw WJ, Bizzarro S, et al. Vitamin C in plasma and leucocytes in relation to periodontitis. J Clin Periodontol 2012; 39(10): 905-12. http://dx.doi.org/10.1111/j.1600-051X.2012.01927.
  19. Wilkinson EG, Arnold RM, Folkers K. Bioenergetics in clinical medicine. VI. adjunctive treatment of periodontal disease with coenzyme Q10. Res Commun Chem Pathol Pharmacol 1976; 14(4): 715-9.
  20. Chang HH, Chang MC, Huang GF, Wang YL, Chan CP, Wang TM, et al. Effect of triethylene glycol dimethacrylate on the cytotoxicity, cyclooxygenase-2 expression and prostanoids production in human dental pulp cells. Int Endod J 2012; 45(9): 848-58. http://dx.doi.org/10.1111/j.1365-2591.2012.02042.
  21. Schweikl H, Altmannberger I, Hanser N, Hiller KA, Bolay C, Brockhoff G, et al. The effect of triethylene glycol dimethacrylate on the cell cycle of mammalian cells. Biomaterials 2005; 26(19): 4111-8. http://dx.doi.org/10.1016/j.biomaterials.2004.10.026.
  22. Janke V, von Neuhoff N, Schlegelberger B, Leyhausen G, Geurtsen W. TEGDMA causes apoptosis in primary human gingival fibroblasts. J Dent Res 2003; 82(10): 814-8. http://dx.doi.org /10.1177/154405910308201010.
  23. Turunen M, Olsson J, Dallner G. Metabolism and function of coenzyme Q. Biochim Biophys Acta 2004; 1660(1-2): 171-99. http://dx.doi.org/10.1016/j.bbamem.2003.11.012.
  24. Hanioka T, Tanaka M, Ojima M, Shizukuishi S, Folkers K. Effect of topical application of coenzyme Q10 on adult periodontitis. Mol Aspects Med 1994; 15: s241-8. https://doi.org/10.1016/0098-2997(94)90034-5
  25. Wang S, Zhang J, Jiang T, Zheng L, Wang Z, Zhang J, et al. Protective effect of Coenzyme Q(10) against oxidative damage in human lens epithelial cells by novel ocular drug carriers. Int J Pharm 2011; 403(1-2): 219-29. http://dx.doi.org/10.1016/j.ijpharm.2010.10.020.
  26. Noh YH, Kim KY, Shim MS, Choi SH, Choi S, Ellisman MH, et al. Inhibition of oxidative stress by coenzyme Q10 increases mitochondrial mass and improves bioenergetic function in optic nerve head astrocytes. Cell Death Dis 2013; 4: e820. http://dx.doi.org/10.1038/cddis.2013.341.
  27. Hans M, Prakash S, Gupta S. Clinical evaluation of topical application of perio-Q gel (Coenzyme Q(10)) in chronic periodontitis patients. J Indian Soc Periodontol 2012; 16(2): 193-9. http://dx.doi.org/10.4103/0972-124X.99261.
  28. Lottner S, Shehata M, Hickel R, Reichl FX, Durner J. Effects of antioxidants on DNA-double strand breaks in human gingival fibroblasts exposed to methacrylate based monomers. Dent Mater 2013; 29(9): 991-8. http://dx.doi.org/10.1016/j.dental.2013.07.005.

Cited by

  1. 사람구강편평상피암세포에서 유지놀에 의한 세포자멸사 유도 효과 vol.15, pp.3, 2014, https://doi.org/10.13065/jksdh.2015.15.03.523
  2. NACA 처리에 따른 치주줄기세포 사멸 억제 효과 vol.8, pp.3, 2020, https://doi.org/10.15268/ksim.2020.8.3.053