Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Protective Effects of Akebia quinata Fruit Extract on Acute Alcohol-induced Hepatotoxicity in Mice

급성 알코올 간독성을 유발한 생쥐에 있어서 으름 열매 추출물의 간 기능 보호효과

  • Lee, Sang Hoon (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University) ;
  • Song, Young Sun (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University) ;
  • Lee, Seo Yeon (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University) ;
  • Kim, So Young (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University) ;
  • Ko, Kwang Suk (Department of Nutritional Science and Food Management, College of Health Science, Ewha Womans University)
  • 이상훈 (이화여자대학교 건강과학대학 식품영양학과) ;
  • 송영선 (이화여자대학교 건강과학대학 식품영양학과) ;
  • 이서연 (이화여자대학교 건강과학대학 식품영양학과) ;
  • 김소영 (이화여자대학교 건강과학대학 식품영양학과) ;
  • 고광석 (이화여자대학교 건강과학대학 식품영양학과)
  • Received : 2014.04.07
  • Accepted : 2014.08.29
  • Published : 2014.10.31
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Abstract

We studied the effects of Akebia quinata fruit extract (AQ) on acute alcohol-induced hepatotoxicity in mice. AQ (30-1,000 mg/kg body weight (BW) per day) was orally administered to the study group, once daily for 1 week. On the last day of AQ treatment, ethanol (6 mg/kg BW) was orally administered to induce acute liver injury. The AQ-treated group showed significantly lower levels of alanine aminotransferase and aspartate aminotransferase, compared to the only ethanol-treated group (ETG). The glutathione level in the AQ-treated group elevated up to 20.6%, compared to that observed in the ETG. The mRNA expression of glutathione synthetic enzymes was also higher in the AQ-treated group, compared to the ETG. The AQ-treated group also exhibited lower levels of expression of NADPH oxidase 4 and tumor necrosis factor alpha mRNA. Thus, these results show that AQ treatment can be a potential method to reduce oxidative stress and inflammation in ethanol-treated mouse liver and also that AQ can be a useful therapeutic agent for acute alcohol-induced hepatotoxicity.

생쥐에서의 으름의 열매 추출물의 경구 투여가 알코올로 인한 급성 간독성 상태에서 간보호 효과에 대한 영향을 조사 하였다. 즉, 생쥐 (C57BL/6)에게 1주간 으름 열매 추출물을 투여 하고 희생 전 알코올의 경구 투여를 통해 급성 알코올 간독성을 유발한 후 간 조직 형상, 간 기능 지표(ALT, AST), 간 세포내 GSH 합성 효소(GCLM, GCLC, GSS)의 mRNA 발현량, GSH 농도의 측정, 산화 스트레스 지표인 NOX4의 mRNA 발현량과 염증 반응 지표인 TNF-${\alpha}$의 mRNA 발현량을 조사 하였다. 그 결과, 으름 열매 추출물의 경구 투여는 알코올로 유발된 급성 간독성 상태에서 간 조직내 지방의 축적을 완화 하였고, 혈청 AST, ALT 수치를 개선하였으며, 간조직 내 항산화 물질인 GSH의 농도를 증가시켰다. 더불어 활성산소기를 생성하는 NOX4의 mRNA 발현을 억제 하는 것으로 분석되었으며 염증 반응 지표인 TNF-의 mRNA 발현도 억제 하는 것으로 분석되었다. 따라서 으름의 열매 추출물은 알코올로 유발된 산화 스트레스, 염증 반응에 대한 간보호 효과 가능성을 나타내는 것으로 판단된다.

Keywords

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