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흑마늘 발효물의 항염증 활성

Anti-inflammatory Activities of Fermented Black Garlic

  • 탁현민 ((재)남해마늘연구소) ;
  • 강민정 ((재)남해마늘연구소) ;
  • 김경민 ((재)남해마늘연구소) ;
  • 강다원 (경상대학교 의학전문대학원 생리학교실) ;
  • 한선규 (경상대학교 의학전문대학원 생리학교실) ;
  • 신정혜 ((재)남해마늘연구소)
  • Tak, Hyun-Min (Namhae Garilic Research Institute) ;
  • Kang, Min-Jung (Namhae Garilic Research Institute) ;
  • Kim, Kyoung Min (Namhae Garilic Research Institute) ;
  • Kang, Dawon (Department of Physiology and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Han, Sunkyu (Department of Physiology and Institute of Health Sciences, Gyeongsang National University School of Medicine) ;
  • Shin, Jung-Hye (Namhae Garilic Research Institute)
  • 투고 : 2014.06.13
  • 심사 : 2014.08.01
  • 발행 : 2014.10.31

초록

Lactobacillus rhamnosus로 발효시킨 흑마늘 발효물의 항염증 효능을 검증하기 위해 LPS로 염증 유도된 RAW 264.7 cells를 이용하여 관련 인자들을 분석하였다. 100, 200, 400 및 $800{\mu}g/mL$ 농도에서 세포독성은 유발되지 않았으며, 오히려 농도 의존적으로 세포 생존율은 증가하였다. LPS에 의해 염증 유도된 RAW 264.7 cells에서 흑마늘 발효물은 농도 의존적으로 NO와 $PGE_2$의 생성 감소와 염증성 사이토카인인 TNF-${\alpha}$, IL-$1{\beta}$ 및 IL-6의 단백질 생성을 감소시켰다. 또한 iNOS, COX-2, NF-${\kappa}B$$I{\kappa}B$ 단백질의 발현을 감소시키고 HO-1의 단백질의 발현을 증가시켰다. 이상의 연구 결과를 통해 흑마늘 발효물은 염증에 의한 NF-${\kappa}B$의 활성과 TNF-${\alpha}$, IL-$1{\beta}$와 IL-6의 생성을 억제시키고, iNOS 및 COX-2의 발현을 억제시키는 메커니즘을 통해 염증성 질환의 예방 및 개선 효능을 나타내는 것으로 판단된다.

In this study, we investigated the anti-inflammatory effects of Lactobacillus rhamnosus fermented black garlic (FBG) in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. FBG did not show cytotoxicity in RAW 264.7 cells at concentrations less than $800{\mu}g/mL$, and cell viability increased with FBG concentration. Nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production as well as tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$) and IL-6 formation decreased in an FBG concentration-dependent manner, in LPS-induced RAW 264.7 cells. Furthermore, activation of LPS-inducible nitric synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor kappa B (NF-${\kappa}B$), and inhibitory kappa B ($I{\kappa}B$) protein expression was effectively inhibited by FBG treatment in LPS-induced RAW 264.7 cells. In contrast, heme oxygenase-1 (HO-1) protein expression significantly increased. These results indicate that the anti-inflammatory activity of FBG was due to activation of NF-${\kappa}B$, inhibition of cytokine production, and expression of iNOS and COX-2. From these results, we expect that FBG could contribute to the prevention and improvement of inflammatory disease.

키워드

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