Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Development of Two-Component Nanorod Complex for Dual-Fluorescence Imaging and siRNA Delivery

  • Choi, Jin-Ha (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Oh, Byung-Keun (Department of Chemical and Biomolecular Engineering, Sogang University)
  • Received : 2014.06.18
  • Accepted : 2014.06.27
  • Published : 2014.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

Recently, multifunctional nanomaterials have been developed as nanotherapeutic agents for cellular imaging and targeted cancer treatment because of their ease of synthesis and low cytotoxicity. In this study, we developed a multifunctional, two-component nanorod consisting of gold (Au) and nickel (Ni) blocks that enables dual-fluorescence imaging and the targeted delivery of small interfering RNA (siRNA) to improve cancer treatment. Fluorescein isothiocyanate-labeled luteinizing hormone-releasing hormone (LHRH) peptides were attached to the surface of a Ni block via a histidine-tagged LHRH interaction to specifically bind to a breast cancer cell line, MCF-7. The Au block was modified with TAMRA-labeled thiolated siRNA in order to knock down the vascular endothelial growth factor protein to inhibit cancer growth. These two-component nanorods actively targeted and internalized into MCF-7 cells to induce apoptosis through RNA interference. This study demonstrates the feasibility of using two-component nanorods as a potential theranostic in breast cancer treatment, with capabilities in dual imaging and targeted gene delivery.

Keywords

References

  1. Agarwal G, Naik RR, Stone MO. 2003. Immobilization of histidine-tagged proteins on nickel by electrochemical dip pen nanolithography. J. Am. Chem. Soc. 125: 7408-7412. https://doi.org/10.1021/ja029856p
  2. Bhattacharyya S, Kudgus RA, Bhattacharya R, Mukherjee P. 2011. Inorganic nanoparticles in cancer therapy. Pharm. Res. 28: 237-259. https://doi.org/10.1007/s11095-010-0318-0
  3. Byrne JD, Betancourt T, Brannon-Peppas L. 2008. Active targeting schemes for nanoparticle systems in cancer therapeutics. Adv. Drug Deliv. Rev. 60: 1615-1626. https://doi.org/10.1016/j.addr.2008.08.005
  4. Chakravarty P, Marches R, Zimmerman NS, Swafford ADE, Bajaj P, Musselman IH, et al. 2008. Thermal ablation of tumor cells with antibody-functionalized single-walled carbon nanotubes. Proc. Natl. Acad. Sci. USA 105: 8697-8702. https://doi.org/10.1073/pnas.0803557105
  5. Chen Y, Zhu X, Zhang X, Liu B, Huang L. 2010. Nanoparticles modified with tumor-targeting scFv deliver siRNA and miRNA for cancer therapy. Mol. Ther. 18: 1650-1656. https://doi.org/10.1038/mt.2010.136
  6. Cho K, Wang XU, Nie S, Shin DM. 2008. Therapeutic nanoparticles for drug delivery in cancer. Clin. Cancer Res. 14: 1310-1316. https://doi.org/10.1158/1078-0432.CCR-07-1441
  7. Choi Y, Choi JH, Liu L, Oh BK, Park S. 2013. Optical sensitivity comparison of multiblock gold-silver nanorods toward biomolecule detection: quadrupole surface plasmonic detection of dopamine. Chem. Mater. 25: 919-926. https://doi.org/10.1021/cm304030r
  8. Devi GR. 2006. siRNA-based approaches in cancer therapy. Cancer Gene Ther. 13: 819-829. https://doi.org/10.1038/sj.cgt.7700931
  9. Dharap SS, Wang Y, Chandna P, Khandare JJ, Qiu B, Gunaseelan S, et al. 2005. Tumor-specific targeting of an anticancer drug delivery system by LHRH peptide. Proc. Natl. Acad. Sci. USA 102: 12962-12967. https://doi.org/10.1073/pnas.0504274102
  10. Filion MC, Phillips NC. 1997. Toxicity and immunomodulatory activity of liposomal vectors formulated with cationic lipids toward immune effector cells. BBA Biomembranes 1329: 345-356 https://doi.org/10.1016/S0005-2736(97)00126-0
  11. Gao X, Cui Y, Levenson RM, Chung LW, Nie S. 2004. In vivo cancer targeting and imaging with semiconductor quantum dots. Nat. Biotechnol. 22: 969-976. https://doi.org/10.1038/nbt994
  12. Howard KA, Rahbek UL, Liu X, Damgaard CK, Glud SZ, Andersen MO, et al. 2006. RNA interference in vitro and in vivo using a chitosan/siRNA nanoparticle system. Mol. Ther. 14: 476-484. https://doi.org/10.1016/j.ymthe.2006.04.010
  13. Huang HC, Barua S, Sharma G, Dey SK, Rege K. 2011. Inorganic nanoparticles for cancer imaging and therapy. J. Control. Release 1553: 344-357.
  14. Huang X, Jain PK, El-Sayed IH, El-Sayed MA. 2007. Gold nanoparticles: interesting optical properties and recent applications in cancer diagnostics and therapy. Nanomedicine 2: 681-693. https://doi.org/10.2217/17435889.2.5.681
  15. Kam NWS, Liu Z, Dai H. 2005. Functionalization of carbon nanotubes via cleavable disulfide bonds for efficient intracellular delivery of siRNA and potent gene silencing. J. Am. Chem. Soc. 127: 12492-12493. https://doi.org/10.1021/ja053962k
  16. Kang Y, Taton TA. 2005. Core/shell gold nanoparticles by self-assembly and crosslinking of micellar, block-copolymer shells. Angew. Chem. Int. Ed. 117: 413-416. https://doi.org/10.1002/ange.200461119
  17. Kim SH, Jeong JH, Lee SH, Kim SW, Park TG. 2008. Local and systemic delivery of VEGF siRNA using polyelectrolyte complex micelles for effective treatment of cancer. J. Control. Release 129: 107-116. https://doi.org/10.1016/j.jconrel.2008.03.008
  18. Liong M, Lu J, Kovochich M, Xia T, Ruehm SG, Nel AE, et al. 2008. Multifunctional inorganic nanoparticles for imaging, targeting, and drug delivery. ACS Nano 2: 889-896. https://doi.org/10.1021/nn800072t
  19. Lofas S, Johnsson BA. 1990. Novel hydrogel matrix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilization of ligands. J. Chem. Soc. Chem. Commun. 21: 1526-1528.
  20. Martiny-Baron G, Marme D. 1995. VEGF-mediated tumour angiogenesis: a new target for cancer therapy. Curr. Opin. Biotechnol. 6: 675-680. https://doi.org/10.1016/0958-1669(95)80111-1
  21. Murphy CJ, Jana NR. 2002. Controlling the aspect ratio of inorganic nanorods and nanowires. Adv. Mater. 14: 80-82. https://doi.org/10.1002/1521-4095(20020104)14:1<80::AID-ADMA80>3.0.CO;2-#
  22. Murphy CJ, Sau TK, Gole AM, Orendorff CJ, Gao J, Gou L, et al. 2005. Anisotropic metal nanoparticles: synthesis, assembly, and optical applications. J. Phys. Chem. B 109: 13857-13870. https://doi.org/10.1021/jp0516846
  23. Park H, Yang J, Lee J, Haam S, Choi IH, Yoo KH. 2009. Multifunctional nanoparticles for combined doxorubicin and photothermal treatments. ACS Nano 3: 2919-2926. https://doi.org/10.1021/nn900215k
  24. Park K, Lee S, Kang E, Kim K, Choi K, Kwon IC. 2009. New generation of multifunctional nanoparticles for cancer imaging and therapy. Adv. Funct. Mater. 19: 1553-1566. https://doi.org/10.1002/adfm.200801655
  25. Peer D, Karp JM, Hong S, Farokhzad OC, Margalit R, Langer R. 2007. Nanocarriers as an emerging platform for cancer therapy. Nat. Nanotechnol. 2: 751-760. https://doi.org/10.1038/nnano.2007.387
  26. Salem AK, Searson PC, Leong KW. 2003. Multifunctional nanorods for gene delivery. Nat. Mater. 2: 668-671. https://doi.org/10.1038/nmat974
  27. Santra S, Kaittanis C, Grimm J, Perez JM. 2009. Drug/dyeloaded, multifunctional iron oxide nanoparticles for combined targeted cancer therapy and dual optical/magnetic resonance imaging. Small 5: 1862-1868. https://doi.org/10.1002/smll.200900389
  28. Schonenberger C, Van der Zande BMI, Fokkink LGJ, Henny M, Schmid C, Krüger M, et al. 1997. Template synthesis of nanowires in porous polycarbonate membranes: electrochemistry and morphology. J. Phys. Chem. B 101: 5497-5505. https://doi.org/10.1021/jp963938g
  29. Sperling RA, Parak WJ. 2010. Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles. Philos. Trans. A Math. Phys. Eng. Sci. 368: 1333-1383. https://doi.org/10.1098/rsta.2009.0273
  30. Thomas CE, Ehrhardt A, Kay MA. 2003. Progress and problems with the use of viral vectors for gene therapy. Nat. Rev. Genet. 4: 346-358. https://doi.org/10.1038/nrg1066
  31. Tsuchida K, Murakami T. 2008. Recent advances in inorganic nanoparticle-based drug delivery systems. Mini-Rev. Med. Chem. 8: 175-183. https://doi.org/10.2174/138955708783498078
  32. Wagner V, Dullaart A, Bock AK, Zweck A. 2006. The emerging nanomedicine landscape. Nat. Biotechnol. 24: 1211-1218. https://doi.org/10.1038/nbt1006-1211
  33. Xu ZP, Zeng QH, Lu GQ, Yu AB. 2006. Inorganic nanoparticles as carriers for efficient cellular delivery. Chem. Eng. Sci. 61: 1027-1040. https://doi.org/10.1016/j.ces.2005.06.019
  34. Yokoyama M. 2005. Drug targeting with nano-sized carrier systems. J. Artif. Organs 8: 77-84. https://doi.org/10.1007/s10047-005-0285-0
  35. Yousefpour P, Atyabi F, Vasheghani-Farahani E, Movahedi AA, Dinarvand R. 2011. Targeted delivery of doxorubicinutilizing chitosan nanoparticles surface-functionalized with anti-Her2 trastuzumab. Int. J. Nanomed. 6: 1977-1990.

Cited by

  1. An overview of synthetic strategies and current applications of gold nanorods in cancer treatment vol.26, pp.43, 2014, https://doi.org/10.1088/0957-4484/26/43/432001
  2. Nanomaterial-based in vitro analytical system for diagnosis and therapy in microfluidic device vol.10, pp.4, 2014, https://doi.org/10.1007/s13206-016-0409-z
  3. Applications, Surface Modification and Functionalization of Nickel Nanorods vol.11, pp.1, 2014, https://doi.org/10.3390/ma11010045
  4. How can nanotechnology help the fight against breast cancer? vol.10, pp.25, 2018, https://doi.org/10.1039/c8nr02796j
  5. Current status and future perspectives of gold nanoparticle vectors for siRNA delivery vol.7, pp.6, 2014, https://doi.org/10.1039/c8tb02484g
  6. Triggered RNAi Therapy Using Metal Inorganic Nanovectors vol.16, pp.8, 2019, https://doi.org/10.1021/acs.molpharmaceut.9b00021
  7. Study on the damage of sperm induced by nickel nanoparticle exposure vol.42, pp.6, 2014, https://doi.org/10.1007/s10653-019-00364-w