Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of Three Dimensional Culture of Porcine Endometrial Cells on Their Plasminogen Activity and Pre-implantation Embryo Development after Co-culture

돼지 자궁 세포의 3차원 배양이 Plasminogen 활성과 수정란 발육에 미치는 영향

  • Lee, Sang-Hee (College of Animal Life Sciences, Division of Applied Animal Science, Kangwon National University) ;
  • HwangBo, Yong (College of Animal Life Sciences, Division of Applied Animal Science, Kangwon National University) ;
  • Cha, Hye-Jin (College of Animal Life Sciences, Division of Applied Animal Science, Kangwon National University) ;
  • Kim, Su-Ji (College of Animal Life Sciences, Division of Applied Animal Science, Kangwon National University) ;
  • Kim, Min-Gyeong (College of Animal Life Sciences, Division of Applied Animal Science, Kangwon National University) ;
  • Cheong, Hee-Tae (College of Veterinary Medicine, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Sciences, Division of Applied Animal Science, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Division of Applied Animal Science, Kangwon National University)
  • 이상희 (강원대학교 동물생명과학대학) ;
  • 황보용 (강원대학교 동물생명과학대학) ;
  • 차혜진 (강원대학교 동물생명과학대학) ;
  • 김수지 (강원대학교 동물생명과학대학) ;
  • 김민경 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의과대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 박춘근 (강원대학교 동물생명과학대학)
  • Received : 2014.08.14
  • Accepted : 2014.09.17
  • Published : 2014.09.30
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Abstract

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in $2.5{\times}10^5cells/ml$ and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of $IL-1{\beta}$ (0.1, 1, 10 and 100 ng/ml) were higher than without $IL-1{\beta}$, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

Keywords

References

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