Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지
DOI QR코드

DOI QR Code

Efficiency of In Vivo Embryo Production following Superovulation with Sex-soted Semen in Hanwoo (Korean Native Cattle)

과배란 처리에 있어 성감별 정액을 이용한 한우 체내 수정란의 생산 효율

  • Jeon, Hyang-A (Animal Genetic Resoures Station, National Institue of Animal Science, RDA) ;
  • Yeom, Gyu-Tae (Animal Genetic Resoures Station, National Institue of Animal Science, RDA) ;
  • Park, Hae-Geum (Animal Genetic Resoures Station, National Institue of Animal Science, RDA) ;
  • Kim, Sung-Woo (Animal Genetic Resoures Station, National Institue of Animal Science, RDA) ;
  • Kim, Hyun (Animal Genetic Resoures Station, National Institue of Animal Science, RDA) ;
  • Kim, Young Sin (Animal Genetic Resoures Station, National Institue of Animal Science, RDA) ;
  • Seong, Hwan-Hoo (Animal Genetic Resoures Station, National Institue of Animal Science, RDA) ;
  • Cho, Young Moo (Animal Genetic Resoures Station, National Institue of Animal Science, RDA) ;
  • Cho, Jae-Hyeon (Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University) ;
  • Ko, Yeoung-Gyu (Animal Genetic Resoures Station, National Institue of Animal Science, RDA)
  • 전향아 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 염규태 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 박해금 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김성우 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김현 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 김영신 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 성환후 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 조영무 (농촌진흥청 국립축산과학원 가축유전자원시험장) ;
  • 조재현 (경상대학교 수의과대학) ;
  • 고응규 (농촌진흥청 국립축산과학원 가축유전자원시험장)
  • Received : 2014.09.15
  • Accepted : 2014.09.18
  • Published : 2014.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Sexed semen is commonly used for the production of calves of the desired gender. Gender selection is important in animal production industries. For example, female cattle are required for the dairy industry while males are preferred in the beef cattle industry. The present study was to assess the in vivo embryo production efficiency using the semen separated according to sex during superovulation in Hanwoo. Seventy Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 days after the third insemination by flushing the uterus with embryo collection medium. KPN semen straws used artificial insemination contained 20 million sperm (total number 60 million per donor). Sex-sorted semen straws contained 4 million sperm (total number 12 million per donor). The results obtained were as follows: No differences were observed in the efficiency of superovulation rates on KPN semen 87%, and sexed semen 100%, respectively. The mean numbers of total embryos are each $12.58{\pm}8.31$ and $13.25{\pm}7.86$. The mean numbers of transferable embryos, sexed semen were significantly lower than KPN semen ($3.75{\pm}1.98$ vs. $8.23{\pm}6.07$, P<0.05). The rates of unfertilized embryos from superovulation using sexed semen were significantly higher than KPN semen (50% vs. 15%, P<0.05). The rate of degenerated 2-cell embryos from sexed and KPN semen was 60.87% and 11.11%, respectively (p<0.05). In conclusion, these results indicate that superovulation using sexed semen was useful, but efficient embryo production was important to reducing the damage caused by the Flowcytometer-based sperm sorting procedure.

Keywords

References

  1. An L, Wu ZH, Wu YF, Zhang XL, Liu X and Zhu YB. 2010. Fertility in single-ovulating and superovulated dairy heifers after insemination with low dose sex-sorted sperm. Reprod. Dom. Anim. 45:344-350. https://doi.org/10.1111/j.1439-0531.2009.01574.x
  2. Chrenek P, Boulanger L, Heyman Y, Uhrin P and Laurincik J. 2001. Sexing and multiple genotype analysis from a single cell of bovine embryo. Theriogenology 55:1071-1081. https://doi.org/10.1016/S0093-691X(01)00467-8
  3. Hayakawa H, Hirai T, Takimoto A, Ideta A and Aoyagi Y. 2009. Superovulation and embryo transfer in Holstein cattle using sexed sperm. Theriogenology 71:68-73. https://doi.org/10.1016/j.theriogenology.2008.09.016
  4. Johnson LA, Flook JP and Hawk HW. 1989. Sex preselection in ravvits:live births from X and Y sperm separated by DNA and cell sorting. Biol. Reprod. 41:199-203. https://doi.org/10.1095/biolreprod41.2.199
  5. Johnson LA. 2000. Sexing mammalian sperm for production of offsring:the state of the art. Anim. Reprod. Sci. 60:93-107.
  6. Kaimio I, Mikkola M, Lindeberg H, Heikkinen J, Hasler JF and Taponen J. 2013. Embryo production with sex-sorted semen in superovulated dairy heifers and cows. Theriogenology 80:950-954. https://doi.org/10.1016/j.theriogenology.2013.07.025
  7. Larson JE, Lamb GC, Funnell BJ, Bird S, Martins A and Rodgers JC. 2010. Embryo production in superovulated Angus cows inseminated four times with sexed-sorted or conventional, frozen-thawed semen. Theriogenology 73:698-703. https://doi.org/10.1016/j.theriogenology.2009.11.009
  8. Mori Y, Nagamine K, Tomita N and Notomi T. 2001. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 289:150-154. https://doi.org/10.1006/bbrc.2001.5921
  9. Morrell JM and Dresser DW. 1989. Offspring from inseminations with mammalian sperm cytometry. Mutat. Res. 224: 177-183. https://doi.org/10.1016/0165-1218(89)90154-7
  10. Morrell JM, Keller KD, Noakes De and Mackenzi NM. 1988. Sexing of sperm by flowcytometry. Vet. Rec. 122:322-324. https://doi.org/10.1136/vr.122.14.322
  11. Pinkel D, Lake S, Gledhill BL, Vandilla MA, Stephenson D and Watchmaker G. 1982. High resolution DNA content measurement of mammalian sperm. Cytometry 3:1-9. https://doi.org/10.1002/cyto.990030103
  12. Peippo J, Vartia K, Kananen-Anttila K, Räty M, Korhonen K and Hurme T. 2009. Embryo production from superovulated Holstein-Friesian dairy heifers and cows after insemination with frozen-thawed sex-sorted X spermatozoa or unsorted semen. Anim. Reprod. Sci. 111:80-92.
  13. Rutledge JJ and Sidel EJ. 1983. Genetic engineering and animal production. J. Anim. Sci. 57:265-272.
  14. Sartori R, Bastos MR and Wiltbank MC. 2010. Factors affecting fertilization and early embryo quality in single- and superovulated dairy cattle. Reprod. Fertil. Dev. 22:151-158. https://doi.org/10.1071/RD09221
  15. Schenk JL, Suh TK and Seidel Jr GE. 2006. Embryo production from superovulated cattle following insemination of sexed sperm. Theriogenology 65:299-307. https://doi.org/10.1016/j.theriogenology.2005.04.026
  16. Stringfellow DA and Seidel SM. 1998. Manual of the International Embryo Transfer Society. 3rd International Embryo Transfer Society Inc Illinois. pp 165-170.
  17. Tubman LM, Brink Z, Suh TK and Seidel GE. Jr. 2004. Characteristics of calves produced with sperm sexed by flow cytometry/cell sorting. J. Anim. Sci. 82:1029-1036. https://doi.org/10.2527/2004.8241029x
  18. 김덕임, 서상원, 정재경, 이규승, 서길웅, 박창식, 정영채, 박병권. 2002. 한우에 있어서 체내 수정란의 생산과 이식에 관한 연구 : 한우 수정란 생산에 영향을 미치는 요인. 한국수정란이식학회지 17:23-32.
  19. 농림수산식품부. 2012. 정자 성 분리 기법에 의한 젖소 암 송아지 생산에 관한 연구. 국회전자도서관. 과천.
  20. 박해금, 김남태, 김성우, 김현, 도윤정, 염규태, 박수봉, 김재환, 김동훈, 조재현, 고응규. 2012. 과배란 처리에 따른 한우와 칡소 체내 수정란 생산 효율과 수정란 이식 수태율. 한국동물번식학회지 36:231-235.
  21. 손동수, 한만희, 최창용, 최선호, 조상래, 김현종, 류일선, 최성복, 이승수, 김영근, 김삼기, 김상희, 신권희, 김일화. 2006. 우수 한우의 수정란 생산 및 이식. 한국수정란이식학회지 21:147-156.
  22. 염규태, 박해금, 김남태, 김성우, 김현, 도윤정, 김영신, 박수봉, 김재환, 조상래, 조재현, 고응규. 2013. 한우에서 신체충실 지수(BCS)가 다배란 처리 시 체내 수정란 생산과 수태율에 미치는 영향. 한국수정란이식학회지 28:7-12.
  23. 조상래, 최선호, 김현종, 한만희, 최창용, 정연길, 손동수. 2005. LAMP 방법에 의한 소 수정란의 성 판별과 Biopsy에 따른 수정란의 체외발달. 한국수정란이식학회지 20:169-176.