Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-Obesity Effect of Ethyl Acetate Fraction from 50% Ethanol Extract of Fermented Curcuma longa L. in 3T3-L1 Cells

발효울금 주정추출물부터 분리된 에틸아세테이트 분획물에 대한 3T3-L1 세포에서의 지방 형성 억제 효과

  • Kim, Jihye (Division of Food and Nutrition, Chonnam National University) ;
  • Park, Jeongjin (Division of Food and Nutrition, Chonnam National University) ;
  • Jun, Woojin (Division of Food and Nutrition, Chonnam National University)
  • 김지혜 (전남대학교 식품영양과학부) ;
  • 박정진 (전남대학교 식품영양과학부) ;
  • 전우진 (전남대학교 식품영양과학부)
  • Received : 2014.10.04
  • Accepted : 2014.10.16
  • Published : 2014.11.30
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Abstract

In the present study, we investigated the effect of ethyl acetate fraction from 50% ethanol extract of fermented Curcuma longa L. (FCEE) on lipid metabolism in 3T3-L1 cells. The safety range of FCEE was up to $300{\mu}g/mL$. Effects of FCEE on lipid accumulation and intracellular triglyceride (TG) content in 3T3-L1 cells were examined by Oil Red O staining and AdipoRed assay. Compared to adipocytes, lipid accumulation and intracellular TG content were significantly reduced by 10.2% and 13.7%, respectively, upon FCEE treatment at a concentration of $200{\mu}g/mL$. Glucose uptake by 3T3-L1 cells was significantly reduced by 36.6% compared to adipocytes at a concentration of $200{\mu}g/mL$. On day 8, free glycerol release into the culture medium was significantly reduced compared to adipocytes at concentrations of 50, 100, and $200{\mu}g/mL$ of FCEE. FCEE significantly stimulated RNA expression of AMP-activated protein kinase (AMPK) and suppressed mRNA expressions of sterol regulatory element-binding protein-1c (SREBP-1c), CCAAT/enhancer binding proteins ${\alpha}$ ($C/EBP{\alpha}$), and peroxisome proliferator- activated receptor ${\gamma}$ ($PPAR{\gamma}$) in 3T3-L1 cells. These results suggest that FCEE inhibits adipogenesis through activation of AMPK mRNA expressions and inhibition of SREBP-1c, $C/EBP{\alpha}$, and $PPAR{\gamma}$ mRNA expressions.

FCEE를 3T3-L1 전지방세포의 분화과정 중에 처리한 후, FCEE가 지방의 축적에 미치는 영향을 확인하였다. FCEE($200{\mu}g/mL$)를 세포에 처리한 후 Oil Red O 염색법과 AdipoRed 형광염색법을 이용한 지방 축적과 세포내 중성지방 함량을 측정한 결과, 무처리한 지방세포와 비교하여 각각 10.2%, 13.7% 유의하게 감소하는 것을 확인하였다. 이러한 결과에 대한 기작을 확인하고자 포도당 유입량과 유리 글리세롤 방출량을 측정하였다. FCEE($200{\mu}g/mL$) 처리 시 지방세포와 비교하여 포도당 유입량이 36.6% 유의적으로 감소하였으며, 유리 글리세롤 방출량도 8일째에 유의적으로 감소하였다. 이는 지방세포의 분화과정 중에 FCEE의 처리로 분화가 억제되어 지방구의 형성이 억제되고 배양액으로 배출되는 유리 글리세롤의 총량이 감소한 것으로 판단된다. 이러한 결과가 지방세포 분화의 억제에 의한 것인지 판단하기 위해 지방 형성 관련인자의 mRNA 발현량을 측정하였다. FCEE($200{\mu}g/mL$)의 처리로 AMPK mRNA 발현량은 지방세포와 비교하여 3배 증가하였으며, SREBP-1c, $C/EBP{\alpha}$와 $PPAR{\gamma}$ mRNA 발현량은 각각 0.6, 0.6 및 0.8배 감소하는 것을 확인하였다. 이상의 결과들로부터 FCEE는 지방 합성을 억제하는 활성을 보유하고 있는 바, 향후 항비만 기능성 식품소재로 활용될 수 있을 것으로 사료된다.

Keywords

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