The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of a PCR Diagnostic System for Iris yellow spot tospovirus in Quarantine

  • Shin, Yong-Gil (Incheon International Airport Regional Office, Animal and Plant Quarantine Agency) ;
  • Rho, Jae-Young (Department of Microbiology, College of Natural Sciences, Dankook University)
  • Received : 2014.06.08
  • Accepted : 2014.08.12
  • Published : 2014.12.01
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Abstract

Iris yellow spot virus (IYSV) is a plant pathogenic virus which has been reported to continuously occur in onion bulbs, allium field crops, seed crops, lisianthus, and irises. In South Korea, IYSV is a "controlled" virus that has not been reported, and inspection is performed when crops of the genus Iris are imported into South Korea. In this study, reverse-transcription polymerase chain reaction (RT-PCR) and nested PCR inspection methods, which can detect IYSV, from imported crops of the genus Iris at quarantine sites, were developed. In addition, a modified positive plasmid, which can be used as a positive control during inspection, was developed. This modified plasmid can facilitate a more accurate inspection by enabling the examination of a laboratory contamination in an inspection system. The inspection methods that were developed in this study are expected to contribute, through the prompt and accurate inspection of IYSV at quarantine sites to the plant quarantine in South Korea.

Keywords

References

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