Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Molecular cloning and characterization of Izumo1 gene from bovine testis

  • Kim, Ekyune (College of Pharmacy, Catholic University of Daegu)
  • Received : 2014.12.03
  • Accepted : 2015.03.19
  • Published : 2015.04.30
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Abstract

A well-characterized sperm specific protein of the Member of immunoglobulin superfamily, IZUMO1, has crucial role in fertilization by mediating sperm binding to the egg plasma membrane in the mouse. However little is known about IZUMO1 in bovine. Here, we describe the molecular cloning and expression analysis of bovine IZUMO1 (bIZUMO1). RT-PCR and Western blot analysis of the bovine tissues indicated that bIZUMO1 was specifically expressed in the testis and sperm, Furthermore, the result of our biotinylation assay from ejaculated bovine sperm strongly suggest the assumption that bIZUMO1 is localized on the cell surface. These data imply the potential role of bovine IZUMO1 in mammalian fertilization.

Keywords

Acknowledgement

Supported by : Catholic University of Daegu

References

  1. Cherr GN, Yudin AI, Overstreet JW. The dual functions of GPI-anchored PH-20: hyaluronidase and intracellular signaling. Matrix Biol. 2001;20:515-25. https://doi.org/10.1016/S0945-053X(01)00171-8
  2. Knobil E, Neill JD. The Physiology of reproduction. 2nd ed. New York: Raven; 1994.
  3. Kim E, Yamashita M, Kimura M, Honda A, Kashiwabara S, Baba T. Sperm penetration through cumulus mass and zona pellucida. Int J Dev Biol. 2008;52:677-82. https://doi.org/10.1387/ijdb.072528ek
  4. Clark S, Naz RK. Presence and incidence of izumo antibodies in sera of immunoinfertile women and men. Am J Reprod Immunol. 2013;69:256-63. https://doi.org/10.1111/aji.12060
  5. Okabe M, Adachi T, Takada K, Oda H, Yagasaki M, Kohama Y, et al. Capacitation-related changes in antigen distribution on mouse sperm heads and its relation to fertilization rate in vitro. J Reprod Immunol. 1987;11:91-100. https://doi.org/10.1016/0165-0378(87)90014-3
  6. Okabe M, Yagasaki M, Oda H, Matzno S, Kohama Y, Mimura T. Effect of a monoclonal anti-mouse sperm antibody (OBF13) on the interaction of mouse sperm with zona-free mouse and hamster eggs. J Reprod Immunol. 1988;13:211-9. https://doi.org/10.1016/0165-0378(88)90002-2
  7. Yamashita M, Yamagata K, Tsumura K, Nakanishi T, Baba T. Acrosome reaction of mouse epididymal sperm on oocyte zona pellucida. J Reprod Dev. 2007;53:255-62. https://doi.org/10.1262/jrd.18145
  8. Inoue N, Ikawa M, Isotani A, Okabe M. The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature. 2005;434:234-8. https://doi.org/10.1038/nature03362
  9. Kim E, Nishimura H, Baba T. Differential localization of ADAM1a and ADAM1b in the endoplasmic reticulum of testicular germ cells and on the surface of epididymal sperm. Biochem Biophys Res Commun. 2003;304:313-9. https://doi.org/10.1016/S0006-291X(03)00588-6
  10. Kim E, Baba D, Kimura M, Yamashita M, Kashiwabara S, Baba T. Identification of a hyaluronidase, Hyal5, involved in penetration of mouse sperm through cumulus mass. Proc Natl Acad Sci U S A. 2005;102:18028-33. https://doi.org/10.1073/pnas.0506825102
  11. Huh JW, Kim YH, Kim DS, Park SJ, Lee SR, Kim SH, et al. Alu-derived old world monkeys exonization event and experimental validation of the LEPR gene. Mol Cells. 2010;30:201-7. https://doi.org/10.1007/s10059-010-0108-x
  12. Kim E, Lee JW, Baek DC, Lee SR, Kim MS, Kim SH, et al. Processing and subcellular localization of ADAM2 in the Macaca fascicularis testis and sperm. Anim Reprod Sci. 2010;117:155-9. https://doi.org/10.1016/j.anireprosci.2009.04.002
  13. Ellerman DA, Pei J, Gupta S, Snell WJ, Myles D, Primakoff P. Izumo is part of a multiprotein family whose members form large complexes on mammalian sperm. Mol Reprod Dev. 2009;76:1188-99. https://doi.org/10.1002/mrd.21092
  14. Kim E, Kim JS, Lee Y, Song BS, Sim BW, Kim SU, et al. Molecular cloning, characterization of porcine IZUMO1, an IgSF family member. Reprod Domest Anim. 2013;48:90-7. https://doi.org/10.1111/j.1439-0531.2012.02037.x
  15. Bazzoni G. The JAM family of junctional adhesion molecules. Curr Opin Cell Biol. 2003;15:525-30. https://doi.org/10.1016/S0955-0674(03)00104-2
  16. Wakayama T, Iseki S. Role of the spermatogenic-Sertoli cell interaction through cell adhesion molecule-1 (CADM1) in spermatogenesis. Anat Sci Int. 2009;84:112-21. https://doi.org/10.1007/s12565-009-0034-1

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