Environmental Engineering Research

  • 제20권4호
  • /
  • Pages.370-376
  • /
  • 2015
  • /
  • 1226-1025(pISSN)
  • /
  • 2005-968X(eISSN)
대한환경공학회 (Korean Society of Environmental Engineers)
권호 표지
DOI QR코드

DOI QR Code

Effects of arsenite and variation of microbial community on continuous bio-hydrogen production from molasses using a sequence batch reactor (SBR)

  • William, Dennis Sambai (Department of Environmental Engineering, Seoul National University of Science and Technology) ;
  • Lee, Pul-eip (Department of Environmental Engineering, Seoul National University of Science and Technology) ;
  • Lee, Tae-jin (Department of Environmental Engineering, Seoul National University of Science and Technology)
  • 투고 : 2015.07.21
  • 심사 : 2015.10.01
  • 발행 : 2015.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study investigated the effects of various arsenite concentrations on bio-hydrogen production from molasses using a sequence batch reactor (SBR) operated in a series of three batch cycles. In the first batch cycle, hydrogen production was stimulated at arsenite concentrations lower than 2.0 mg/L, while inhibition occurred at arsenite concentration higher than 2.0 mg/L compared to the control. Hydrogen production decreased substantially during the second batch cycle, while no hydrogen was produced during the third batch cycle at all tested concentrations. The toxic density increased with respect to the increase in arsenite concentrations (6.0 > 1.6 > 1.0 > 0.5 mg/L) and operation cycles (third cycle > second cycle > first cycle). The presence of microorganisms such as Clostridium sp. MSTE9, Uncultured Dysgonomonas sp. clone MEC-4, Pseudomonas parafulva FS04, and Uncultured bacterium clone 584CL3e9 resulted in active stimulation of hydrogen production, however, it was unlikely that Enterobacter sp. sed221 was not related to hydrogen production. The tolerance of arsenite in hydrogen producing microorganisms decreased with the increase in induction time, which resulted in severing the inhibition of continuous hydrogen production.

키워드

참고문헌

  1. Das D, Veziroglu TN. Hydrogen production by biological processes: a survey of literature. Int. J. Hydrogen Energ. 2001;26:13-28. https://doi.org/10.1016/S0360-3199(00)00058-6
  2. Chong ML, Sabaratnam V, Shirai Y, Hassan MA. Biohydrogen production from biomass and industrial wastes by dark fermentation. Int. J. Hydrogen Energ. 2009;34:3277-3287. https://doi.org/10.1016/j.ijhydene.2009.02.010
  3. Momirlan M, Veziroglu TN. Current status of hydrogen energy. Renew Sust. Energ. Rev. 2002;6:141-179. https://doi.org/10.1016/S1364-0321(02)00004-7
  4. Hallenbeck PC, Ghosh D. Advances in fermentative biohydrogen production: the way forward? Trends Biotechnol. 2009;27:287-297. https://doi.org/10.1016/j.tibtech.2009.02.004
  5. Das D, Veziroglu TN. Advances in biological hydrogen production processes. Int. J. Hydrogen Energ. 2008;33:6046-6057. https://doi.org/10.1016/j.ijhydene.2008.07.098
  6. Teclu D, Tivchev G, Laing M, Wallis M. Determination of the elemental composition of molasses and its suitability as carbon source for growth of sulphate-reducing bacteria. J. Hazard. Mater. 2009;161:1157-1165. https://doi.org/10.1016/j.jhazmat.2008.04.120
  7. Chung J-Y, Yu S-D, Hong Y-S. Environmental source of arsenic exposure. J. Prev. Med. Public Health 2014;47:253. https://doi.org/10.3961/jpmph.14.036
  8. Cho Y, Lee T. Variations of hydrogen production and microbial community with heavy metals during fermentative hydrogen production. J. Industrial Eng. Chem. 2011;17:340-345. https://doi.org/10.1016/j.jiec.2011.02.036
  9. Pedro MS, Haruta S, Hazaka M, et al. Denaturing gradient gel electrophoresis analyses of microbial community from field-scale composter. J. Biosci. Bioeng. 2001;91:159-165. https://doi.org/10.1016/S1389-1723(01)80059-1
  10. Cocolin L, Aggio D, Manzano M, Cantoni C, Comi G. An application of PCR-DGGE analysis to profile the yeast populations in raw milk. Int. Dairy J. 2002;12:407-411. https://doi.org/10.1016/S0958-6946(02)00023-7
  11. Mizuno O, Dinsdale R, Hawkes FR, Hawkes DL, Noike T. Enhancement of hydrogen production from glucose by nitrogen gas sparging. Bioresour. Technol. 2000;73:59-65. https://doi.org/10.1016/S0960-8524(99)00130-3
  12. Jun Y-S, Yu S-H, Ryu K-G, Lee T-J. Kinetic study of pH effects on biological hydrogen production by a mixed culture. J. Microbiol. Biotechnol. 2008;18:1130-1135.
  13. APHA. Standard Methods for the Examination of Water and Wastewater. Washington: American public Health Association; 2002.
  14. Dubois M, Gilles KA, Hamilton JK, Rebers P, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1956;28:350-356. https://doi.org/10.1021/ac60111a017
  15. Lee YJ, Miyahara T, Noike T. Effect of pH on microbial hydrogen fermentation. J. Chem. Technol. Biotechnol. 2002;77:694-698. https://doi.org/10.1002/jctb.623
  16. Lay J-J, Li Y-Y, Noike T. Influences of pH and moisture content on the methane production in high-solids sludge digestion. Water. Res. 1997;31:1518-1524. https://doi.org/10.1016/S0043-1354(96)00413-7
  17. Mudhoo A, Kumar S. Effects of heavy metals as stress factors on anaerobic digestion processes and biogas production from biomass. Int. J. Environ. Sci. Technol. 2013;10:1383-1398. https://doi.org/10.1007/s13762-012-0167-y
  18. Li CL, Fang HHP. Inhibition of heavy metals on fermentative hydrogen production by granular sludge. Chemosphere 2007;67:668-673. https://doi.org/10.1016/j.chemosphere.2006.11.005
  19. Wei H, Bing W, Xiaoye L, Chunyu L, Liran Y, Yongfeng L. Fermentative hydrogen production from molasses in an activated sludge immobilized bioreactor. Int. J. Energy Eng. 2012.
  20. Li CL, Fang HHP. Fermentative hydrogen production from wastewater and solid wastes by mixed cultures. Crit. Rev. Env. Sci. Tec. 2007;37:1-39. https://doi.org/10.1080/10643380600729071
  21. Hallenbeck PC, Abo-Hashesh M, Ghosh D. Strategies for improving biological hydrogen production. Bioresour. Technol. 2012;110:1-9. https://doi.org/10.1016/j.biortech.2012.01.103
  22. Won SG, Baldwin SA, Lau AK, Rezadehbashi M. Optimal operational conditions for biohydrogen production from sugar refinery wastewater in an ASBR. Int. J. Hydrogen Energ. 2013;38:13895-13906. https://doi.org/10.1016/j.ijhydene.2013.08.071
  23. Wang JL, Wan W. Factors influencing fermentative hydrogen production: A review. Int. J. Hydrogen Energ. 2009;34:799-811. https://doi.org/10.1016/j.ijhydene.2008.11.015
  24. Das D. Advances in biohydrogen production processes: An approach towards commercialization. Int. J. Hydrogen Energ. 2009;34:7349-7357. https://doi.org/10.1016/j.ijhydene.2008.12.013
  25. Tsai SL, Singh S, Chen W. Arsenic metabolism by microbes in nature and the impact on arsenic remediation. Curr. Opin. Biotechnol. 2009;20:659-667. https://doi.org/10.1016/j.copbio.2009.09.013
  26. Aydin YA, Aksoy ND. Isolation and characterization of an efficient bacterial cellulose producer strain in agitated culture: Gluconacetobacter hansenii P2A. Appl. Microbiol. Biotechnol. 2014;98:1065-1075. https://doi.org/10.1007/s00253-013-5296-9
  27. Giller KE, Witter E, McGrath SP. Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils: A review. Soil Biol. Biochem. 1998;30:1389-1414. https://doi.org/10.1016/S0038-0717(97)00270-8

피인용 문헌

  1. Enhanced Bio-hydrogen Production from Pretreated Microalgal Waste vol.41, pp.9, 2015, https://doi.org/10.4491/ksee.2019.41.9.494