한국버섯학회지 (Journal of Mushroom)

  • 제13권4호
  • /
  • Pages.314-318
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  • 2015
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  • 1738-0294(pISSN)
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  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
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감과피를 이용한 큰느타리버섯 배지 개발

Development of medium for Pleurotus eryngii cultivation using Kojongsi persimmon peels

  • 김철환 (경남과학기술대학교 제약공학과) ;
  • 김혜수 (경남과학기술대학교 제약공학과) ;
  • 김홍출 (경남과학기술대학교 제약공학과) ;
  • 권현숙 (한국한방산업진흥원) ;
  • 정종천 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 조수정 (경남과학기술대학교 제약공학과)
  • Kim, Chul Hwan (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kim, Hye Soo (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kim, Hong Chul (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Kwon, Hyun Sook (Korea Promotion Institute for Traditional Medicine Industry) ;
  • Cheong, Jong-Chun (Mushroom Research Division, International Institute of Horticultural & Herhal Science, RDA) ;
  • Kong, Won-Sik (Mushroom Research Division, International Institute of Horticultural & Herhal Science, RDA) ;
  • Cho, Soo Jeong (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 투고 : 2015.11.12
  • 심사 : 2015.12.22
  • 발행 : 2015.12.31
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초록

본 연구는 지역 특산물인 감과피를 이용한 버섯배지 개발을 위한 기초자료로서 감과피의 이용 가능성을 검토하고자 수행되었으며 감과피는 경상남도 산청지역에서 곶감을 생산하고 남은 것을 수거하여 건조한 후 사용하였고 공시균주는 ASI 2312를 사용하였다. 대조구는 혼합배지:콘코브:톱밥(220:65:15, v/v)을 혼합한 기본배지를 사용하였고 기본배지 중 혼합배지를 5%, 10%, 15%, 20%, 30%, 40%, 50%의 건조된 감과피 처리구로 대체한 처리구를 시험구로 사용하였다. 대조구인 기본배지에 비해 시험구인 5%, 10%, 15%, 20%, 30%, 40%, 50% 감과피 처리구의 T-N 함량은 감과피 첨가량이 증가할수록 감소하는 경향을 나타내었으며 T-C 함량은 큰 차이가 없었고 C/N율은 감과피 첨가량이 증가할수록 증가하는 경향을 나타내었다. 감과피 첨가량이 균사생장에 미치는 영향을 조사한 결과 대조구보다 감과피가 첨가된 모든 시험구에서 균사생장이 느리게 나타났다. 감과피 첨가량에 따른 배양일수는 대조구인 혼합배지에 비해 감과피를 30%까지 첨가하여도 배양일수와 초발이 소요일수에는 뚜렷한 차이는 없었지만 감과피 첨가량이 증가할수록 배양일이 길어지는 경향을 보였으며 혼합배지에 비해 감과피 첨가비율이 증가할수록 갓의 직경은 감소하고 대의 길이는 길어지고 직경은 감소하는 경향을 나타내었다. 수확 후 동결 건조한 큰느타리버섯의 수분, 단백질, 조회분 함량은 대조구와 시험구에서 유의성 있는 차이를 나타내지 않았지만 조지방 함량은 감과피 첨가량이 증가함에 따라 증가하는 경향을 나타내었고 ${\beta}-glucan$ 함량은 대조구에 비해 10%와 15% 감과피 첨가구에서 높게 나타났다. 따라서 대조구와 비교했을 때 15% 감과피 첨가구는 자실체 수량도 높게 나타나고 ${\beta}-glucan$ 함량도 높게 나타났기때문에 감과피 첨가비율은 15%가 가장 적합한 것으로 사료된다.

This study was carried out to investigated the avaiability of dried Koojongsi persimmon peels (KPP) as a useful mushroom medium using Pleurotus eryngii ASI 2312. Mushroom cultivation medium used in this study was mixed with medium mixture, corn cob and sawdust (220:65:15, v/v). Dried KPP was replaced mushroom cultivation medium (control) with 5, 10, 15, 20, 30, 40, 50% dried KPP. The T-N content of dried KPP treatments decreased to increase replaced ratio of the dried KPP and C/N ratio was increased to increase replaced ratio of the dried KPP. But T-C content of dried KPP treatments was similar to untreated control. The average cultivating periods of mycelium on dried KPP treatments was delayed to increase replaced ratio of the dried KPP and cultivating periods was delayed over 30% dried KPP treatments. The length of stipe of dried KPP treatments was longer than that of the untreated control to increase replaced ratio of the dried KPP and thickness of stipes was tend to be thinner than that of the untreated control to increase replaced ratio of the dried KPP. The moisture, carbohydrate, crude protein and crude ash content of mycelial were similar to untreated control, but crude fatty acid was increased to increase replaced ratio of the dried KPP. The ${\beta}-glucan$ content of 10% and 15% treatments were higher than untreatment control. The results based on cultivation yield and ${\beta}-glucan$ content indicated that optimal mixture ratio dried KPP was 15%.

키워드

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