Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Anti-thrombotic activity of fermented rice bran extract with several oriental plants in vitro and in vivo

쌀겨발효추출물의 항혈전효과

  • Jeon, Bo-Ra (Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University) ;
  • Ji, Hyun Dong (Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Su Jung (Department of Clinical Pathology, Daegu Health College) ;
  • Lee, Chun-Hee (Enzyme LAPA Co.) ;
  • Kim, Tae-Wan (Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University) ;
  • Rhee, Man-Hee (Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University)
  • Received : 2015.09.14
  • Accepted : 2015.11.25
  • Published : 2015.12.31
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Abstract

Although the effects of the rice bran have recently been investigated, there is no information regarding platelet physiology available. However, it is well known that fermented natural plants have a beneficial effect on cardiovascular diseases. Therefore, this study was conducted to investigate whether fermented rice bran extract (FRBE) with several plants (Artemisia princeps, Angelica Gigantis Radix, Cnidium officinale, and Camellia sinensis) affected agonist-induced platelet aggregation, and if so, what the underlying mechanism of its activity was. We performed several experiments, including in vitro platelet aggregation, intracellular calcium concentration and adenosine triphosphate release. In addition, the activation of integrin ${\alpha}_{II}b{\beta}3$ was determined using fibrinogen binding. Thrombus formation was also evaluated in vivo using an arterio-venous shunt model. The FRBE inhibited collagen-induced platelet aggregation in a concentration-dependent manner. FRBE significantly and dose dependently attenuated thrombus formation using rat arterio-venous shunt. FRBE suppressed the intracellular calcium mobilization in collagen-stimulated platelets. We also found that FRBE inhibited extracellular stimuli-responsive kinase 1/2, p38-mitogen-activated protein kinases and c-Jun N-terminal kinase phosphorylation. These results suggested that FRBE inhibited collagen-induced platelet aggregation, which was mediated by modulation of downstream signaling molecules. In conclusion, FRBE could be developed as a functional food against aberrant platelet activation-related cardiovascular diseases.

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References

  1. Adam F, Kauskot A, Rosa JP, Bryckaert M. Mitogenactivated protein kinases in hemostasis and thrombosis. J Thromb Haemost 2008, 6, 2007-2016. https://doi.org/10.1111/j.1538-7836.2008.03169.x
  2. Berridge MJ, Irvine RF. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature 1984, 312, 315-321. https://doi.org/10.1038/312315a0
  3. Bhatt DL, Topol EJ. Scientific and therapeutic advances in antiplatelet therapy. Nat Rev Drug Discov 2003, 2, 15-28. https://doi.org/10.1038/nrd985
  4. Cho JI, Jung HJ, Ha SD, Kim KS. Growth patterns of lactic acid bacteria during fermentation of radish with rice water and rice bran. Korean J Food Sci Technol 2004, 36, 837-841.
  5. Cho SK, Kwon OI, Kim CJ. Anti-inflammatory and analgesic activities of the extracts and fractions of Cnidii rhizoma. Korean J Pharmacogn 1996, 27, 282-288.
  6. Cho SY, Park JY, Oh YJ, Jang JY, Park EM, Kim MJ, Kim KS. Effect of dandelion leaf extracts on lipid metabolism in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 2000, 29, 676-682.
  7. Choi HI, Lee BK, Kim SJ. Study on the nutritional components of non-fermented rice bran and fermented rice bran. Korean J Food Nutr 2010, 23, 1-7.
  8. Choi HI, Ye EJ, Kim SJ, Bae MJ, Yee ST, Park EJ, Park EM. Anticancer (in vitro) and antiallergy effects of rice bran extracts. J Korean Soc Food Sci Nutr 2006, 35, 1297-1303. https://doi.org/10.3746/jkfn.2006.35.10.1297
  9. Gum PA, Kottke-Marchant K, Welsh PA, White J, Topol EJ. A prospective, blinded determination of the natural history of aspirin resistance among stable patients with cardiovascular disease. J Am Coll Cardiol. 2003, 41, 961-965. https://doi.org/10.1016/S0735-1097(02)03014-0
  10. Hu FB. Plant-based foods and prevention of cardiovascular disease: an overview. Am J Clin Nutr 2003, 78 (Suppl), 544S-551S. https://doi.org/10.1093/ajcn/78.3.544S
  11. Ishikawa T, Suzukawa M, Ito T, Yoshida H, Ayaori M, Nishiwaki M, Yonemura A, Hara Y, Nakamura H. Effect of tea flavonoid supplementation on the susceptibility of lowdensity lipoprotein to oxidative modification. Am J Clin Nutr 1997, 66, 261-266. https://doi.org/10.1093/ajcn/66.2.261
  12. Israels SJ, Gerrard JM, Jacques YV, McNicol A, Cham B, Nishibori M, Bainton DF. Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140). Blood 1992, 80, 143-152.
  13. Jennings LK. Role of platelets in atherothrombosis. Am J Cardiol 2009, 103 (Suppl), 4A-10A. https://doi.org/10.1016/S0002-9149(08)02058-4
  14. Kang S, Han J, Jang K. DPPH radical scavenger activity and antioxidant effects of Cham-Dang-Gui (Angelica gigas). J Korean Soc Food Sci Nutr 2004, 33, 1112-1118. https://doi.org/10.3746/jkfn.2004.33.7.1112
  15. Kim SD, Lee IK, Lee WM, Cho JY, Park HJ, Oh JW, Park SC, Kim SK, Kwak YS, Yun BS, Rhee MH. The mechanism of anti-platelet activity of davallialactone: involvement of intracellular calcium ions, extracellular signalregulated kinase 2 and p38 mitogen-activated protein kinase. Eur J Pharmacol 2008, 584, 361-367. https://doi.org/10.1016/j.ejphar.2008.02.008
  16. Kuk YI, Shin JS, Kwon OD, Guh JO. Effect of aqueous extracts of rice bran on inhibition of germination and early growth of weeds. Korean J Environ Agric 2001, 20, 108-111.
  17. Lee JH, Choi HS, Chung MS, Lee MS. Volatile flavor components and free radical scavenging activity of Cnidium officinale. Korean J Food Sci Technol 2002, 34, 330-338.
  18. Lee SS, Lee MJ, Kim BJ, Hong SB. Growth, yield and grain quality of rice affected by application of crab shell, sericite ore, and charcoal powders. Korean J Environ Agric 2005, 24, 185-190. https://doi.org/10.5338/KJEA.2005.24.2.185
  19. Lee YY, Lee S, Jin JL, Yun-Choi HS. Platelet antiaggregatory effects of coumarins from the roots of Angelica genuflexa and A. gigas. Arch Pharm Res 2003, 26, 723-726. https://doi.org/10.1007/BF02976681
  20. Lim SS, Lee JH. Biological activity of the soluble extracts from Artemisia princeps var orientalis acted on cardiovascular system. Korean J Nutr 1997, 30, 634-638.
  21. Mohan KVP, Gunasekaran P, Varalakshmi E, Hara Y, Nagini S. In vitro evaluation of the anticancer effect of lactoferrin and tea polyphenol combination on oral carcinoma cells. Cell Biol Int 2007, 31, 599-608. https://doi.org/10.1016/j.cellbi.2006.11.034
  22. Most MM, Tulley R, Morales S, Lefevre M. Rice bran oil, not fiber, lowers cholesterol in humans. Am J Clin Nutr 2005, 81, 64-68. https://doi.org/10.1093/ajcn/81.1.64
  23. Nishikawa M, Tanaka T, Hidaka H. $Ca^{2+}$-calmodulindependent phosphorylation and platelet secretion. Nature 1980, 287, 863-865. https://doi.org/10.1038/287863a0
  24. Oh WJ, Endale M, Park SC, Cho JY, Rhee MH. Dual roles of quercetin in platelets: phosphoinositide-3-kinase and MAP kinases inhibition, and cAMP-dependent vasodilator-stimulated phosphoprotein stimulation. Evid Based Complement Alternat Med 2012, 2012, 485262.
  25. Oury C, Toth-Zsamboki E, Vermylen J, Hoylaerts MF. P2X1-mediated activation of extracellular signal-regulated kinase 2 contributes to platelet secretion and aggregation induced by collagen. Blood 2002, 100, 2499-2505. https://doi.org/10.1182/blood-2002-03-0812
  26. Park JH, Ha AW, Cho JS. Effects of green tea-soybean paste on weights and serum lipid profiles in rats fed high fat diet. Korean J Food Sci Technol 2005, 37, 806-811.
  27. Park KW, Choi SR, Shon MY, Jeong IY, Kang KS, Lee ST, Shim KH, Seo KI. Cytotoxic effects of decursin from Angelica gigas Nakai in human cancer cells. J Korean Soc Food Sci Nutr 2007, 36, 1385-1390. https://doi.org/10.3746/jkfn.2007.36.11.1385
  28. Pasqui AL, Capecchi PL, Ceccatelli L, Mazza S, Gistri A, Laghi Pasini F, Di Perri T. Nitroprusside in vitro inhibits platelet aggregation and intracellular calcium translocation. Effect of haemoglobin. Thromb Res 1991, 61, 113-122.
  29. Patrono C, Coller B, FitzGerald GA, Hirsh J, Roth G. Platelet-active drugs: the relationships among dose, effectiveness, and side effects: the Seventh ACCP Conference on Antithrombotic and Thrombolytic Therapy. Chest 2004, 126 (3 Suppl), 234S-264S. https://doi.org/10.1378/chest.126.3_suppl.234S
  30. Schaeffer J, Blaustein MP. Platelet free calcium concentrations measured with fura-2 are influenced by the transmembrane sodium gradient. Cell Calcium 1989, 10, 101-113. https://doi.org/10.1016/0143-4160(89)90050-X
  31. Schwartz SM, Heimark RL, Majesky MW. Developmental mechanisms underlying pathology of arteries. Physiol Rev 1990, 70, 1177-1209. https://doi.org/10.1152/physrev.1990.70.4.1177
  32. Shin S, Jeon JH, Park D, Jang JY, Joo SS, Hwang BY, Choe SY, Kim YB. Anti-inflammatory effects of an ethanol extract of Angelica gigas in a Carrageenan-air pouch inflammation model. Exp Anim 2009, 58, 431-436. https://doi.org/10.1538/expanim.58.431
  33. Sohn HY, Kwon CS, Son KH, Kwon GS, Kwon YS, Ryu HY, Kum EJ. Antithrombosis and antioxidant activity of methanol extract from different brands of rice. J Korean Soc Food Sci Nutr 2005, 34, 593-598. https://doi.org/10.3746/jkfn.2005.34.5.593
  34. Umetsu T, Sanai K. Effect of 1-methyl-2-mercapto-5-(3-pyridyl)-imidazole (KC-6141), an anti-aggregating compound, on experimental thrombosis in rats. Thromb Haemost 1978, 39, 74-83.
  35. Unsworth AJ, Smith H, Gissen P, Watson SP, Pears CJ. Submaximal inhibition of protein kinase C restores ADPinduced dense granule secretion in platelets in the presence of $Ca^{2+}$. J Biol Chem 2011, 286, 21073-21082. https://doi.org/10.1074/jbc.M110.187138
  36. Wang Y, Li Q, Wang Q, Li Y, Ling J, Liu L, Chen X, Bi K. Simultaneous determination of seven bioactive components in Oolong tea Camellia sinensis: quality control by chemical composition and HPLC fingerprints. J Agric Food Chem 2012, 60, 256-260. https://doi.org/10.1021/jf204312w
  37. Yang KS, Jeon CM. Effect of Taraxacum coreanum Nakai on low density lipoprotein oxidation. Korean J Pharmacogn 1996, 27, 267-273.
  38. Zhang L, Du JR, Wang J, Yu DK, Chen YS, He Y, Wang CY. Z-ligustilide extracted from Radix Angelica Sinensis decreased platelet aggregation induced by ADP ex vivo and arterio-venous shunt thrombosis in vivo in rats. Yakugaku Zasshi 2009, 129, 855-859. https://doi.org/10.1248/yakushi.129.855