Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Confirmation of Two Undescribed Fungal Species from Dokdo of Korea Based on Current Classification System Using Multi Loci

  • Lee, Hye Won (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Nguyen, Thi Thuong Thuong (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Yeon, Hye (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Lee, Haengsub (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University) ;
  • Kim, Changmu (National Institute of Biological Resources) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology & Agrochemistry, College of Agriculture & Life Sciences, Chonnam National University)
  • Received : 2015.08.20
  • Accepted : 2015.10.26
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Using dilution plating method, 47 fungal isolates were obtained from a soil sample collected from Dokdo in the East Sea of Korea in 2013. In this study, two fungal isolates, EML-MFS30-1 and EML-DDSF4, were confirmed as undescribed species, Metarhizium guizhouense and Mortierella oligospora in Korea based on current classification system using multi loci including rDNA internal transcribed spacer, large subunit, small subunit, and ${\beta}$-tubulin (BTUB) genes. Herein, detailed morphological descriptions on characters of the undescribed fungal species as well as their molecular phylogenetic status are provided with comparisons to related species.

Keywords

References

  1. Ryu SH, Jang KH, Choi EH, Kim SK, Song SJ, Cho HJ, Ryu JS, Kim YM, Sagong J, Lee JH, et al. Biodiversity of marine invertebrates on rocky shores of Dokdo, Korea. Zool Stud 2012;51:710-26.
  2. Elmer WH, Marra RE. New species of Fusarium associated with dieback of Spartina alterniflora in Atlantic salt marshes. Mycologia 2011;103:806-19. https://doi.org/10.3852/10-155
  3. Mandeel QA. Biodiversity of the genus Fusarium in saline soil habitats. J Basic Microbiol 2006;46:480-94. https://doi.org/10.1002/jobm.200510128
  4. Pang KL, Jheng JS. Pileomyces formosanus gen. et sp. nov. (Halosphaeriaceae, Ascomycota) from a rocky shore of Taiwan. Bot Stud 2012;53:535-9.
  5. Nayak SS, Gonsalves V, Nazareth SW. Isolation and salt tolerance of halophilic fungi from mangroves and solar salterns in Goa-India. Indian J Geomarine Sci 2012;41:164-72.
  6. Rogers DP. Fungi of the Marshall islands, central Pacific Ocean. Pac Sci 1947;1:92-107.
  7. Humber RA, Rombach MC. Torrubiella ratticaudata sp. nov. (Pyrenomycetes: Clavicipitales) and other fungi from spiders on the Solomon Islands. Mycologia 1987;79:375-82. https://doi.org/10.2307/3807459
  8. Alias SA, Jones EB, Torres J. Intertidal fungi from the Philippines, with a description of Acrocordiopsis sphaerica sp. nov. (Ascomycota). Fungal Divers 1999;2:35-41.
  9. Sorokin N. Rastitelnye parazity cheloveka i zhivotnykn' kak' prichina zaraznykn' boleznei (Plant parasites causing infectious diseases of man and animals). Vyp. II.Pervoe prilozenie k Voenno-Meditsinskomu Zhurnalu za 1883 g (First supplement to J Military Med 1883). St. Petersburg: Izdanie glavnogo Voenno-Meditsinskago Upraveleneia; 1883. p. 168-98.
  10. Tulloch M. The genus Metarhizium. Trans Br Mycol Soc 1976;66:407-11. https://doi.org/10.1016/S0007-1536(76)80209-4
  11. Benny GL. Zygomycetes [Internet]. Gerald L. Benny; 2009 [cited 2015 Oct 1]. Available from: http://www.zygomycetes.org.
  12. You YH, Yoon H, Lee GS, Woo JR, Shin JH, Lee IJ, Rim SO, Choo YS, Kim JG. Diversity and plant growth-promotion of endophytic fungi isolated from the roots of plants in Dokdo islands. J Life Sci 2011;21:992-6. https://doi.org/10.5352/JLS.2011.21.7.992
  13. You YH, Yoon H, Kim H, Lim SH, Shin JH, Lee IJ, Choo YS, Kim JG. Plant growth-promoting activity and genetic diversity of endophytic fungi isolated from native plants in Dokdo islands for restoration of a coastal ecosystem. J Life Sci 2013;23:95-101. https://doi.org/10.5352/JLS.2013.23.1.95
  14. Yoon JH, Kang SJ, Lee SY, Lee MH, Oh TK. Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korea. Int J Syst Evol Microbiol 2005;55(Pt 5):1833-7. https://doi.org/10.1099/ijs.0.63613-0
  15. Lee CM, Weon HY, Yoon SH, Kim SJ, Koo BS, Kwon SW. Burkholderia denitrificans sp. nov., isolated from the soil of Dokdo Island, Korea. J Microbiol 2012;50:855-9. https://doi.org/10.1007/s12275-012-1554-2
  16. Wagner L, Stielow B, Hoffmann K, Petkovits T, Papp T, Vagvolgyi C, de Hoog GS, Verkley G, Voigt K. A comprehensive molecular phylogeny of the Mortierellales (Mortierellomycotina) based on nuclear ribosomal DNA. Persoonia 2013;30:77-93. https://doi.org/10.3767/003158513X666268
  17. Bischoff JF, Rehner SA, Humber RA. A multilocus phylogeny of the Metarhizium anisopliae lineage. Mycologia 2009;101: 512-30. https://doi.org/10.3852/07-202
  18. Petkovits T, Nagy LG, Hoffmann K, Wagner L, Nyilasi I, Griebel T, Schnabelrauch D, Vogel H, Voigt K, Vagvolgyi C, et al. Data partitions, Bayesian analysis and phylogeny of the zygomycetous fungal family Mortierellaceae, inferred from nuclear ribosomal DNA sequences. PLoS One 2011;6: e27507. https://doi.org/10.1371/journal.pone.0027507
  19. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95-8.
  20. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876-82. https://doi.org/10.1093/nar/25.24.4876
  21. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007;24:1596-9. https://doi.org/10.1093/molbev/msm092
  22. Yadav DR, Kim SW, Babu AG, Adhikari M, Kim C, Lee HB, Lee YS. First report of Mortierella alpina (Mortierellaceae, Zygomycota) isolated from crop field soil in Korea. Mycobiology 2014;42:401-4. https://doi.org/10.5941/MYCO.2014.42.4.401
  23. Mehrotra BS, Mehrotra BR, Baijal U. Species of Mortierella from India: V. Mortierella oligospora Bjorling and M. oligospora var. indica var. nov. Sydowia 1963;17:186-9.
  24. Walter M, Ford CI, Guthrie JV, Boyd-Wilson KS, Di HJ, Cameron KC, Parker E. Bioremediation of aged pentachlorophenol residues by white-rot fungi. In: Rai M, editor. Mycotechnology: present status and future prospects. New Delhi: I. K. International Publishing House Pvt. Ltd.; 2007. p. 18-54.
  25. Kataoka R, Takagi K, Sakakibara F. A new endosulfandegrading fungus, Mortierella species, isolated from a soil contaminated with organochlorine pesticides. J Pestic Sci 2010; 35:326-32. https://doi.org/10.1584/jpestics.G10-10
  26. Sung GH, Hywel-Jones NL, Sung JM, Luangsa-ard JJ, Shrestha B, Spatafora JW. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud Mycol 2007;57:5-59. https://doi.org/10.3114/sim.2007.57.01
  27. Maniania NK, Sithanantham S, Ekesi S, Ampong-Nyarko K, Baumgartner J, Lohr B, Matoka CM. A field trial of the entomogenous fungus Metarhizium anisopliae for control of onion thrips, Thrips tabaci. Crop Prot 2003;22:553-9. https://doi.org/10.1016/S0261-2194(02)00221-1
  28. Yang L, Zhang T, Li XH, Yu R. Purification of a novel chitosanase from Metarhizium guizhouense in solid-state fermentation with affinity chromatography. Sichuan Da Xue Xue Bao Yi Xue Ban 2007;38:713-6.
  29. Ouedraogo A, Fargues J, Goettel MS, Lomer CJ. Effect of temperature on vegetative growth among isolates of Metarhizium anisopliae and M. flavoviride. Mycopathologia 1997;137:37-43. https://doi.org/10.1023/A:1006882621776
  30. Wang C, Skrobek A, Butt TM. Investigations on the destruxin production of the entomopathogenic fungus Metarhizium anisopliae. J Invertebr Pathol 2003;85:168-74.
  31. Lomer CJ, Prior C, Kooyman C. Development of Metarhizium spp. for the control of grasshopper and locusts. Mem Entomol Soc Can 1997;129(Suppl S171):265-86. https://doi.org/10.4039/entm129171265-1
  32. Filippovich SY, Rybakov YA, Afanasieva TP, Bachurina GP, Lukina GP, Ezhova IE, Nosova AV, Artjushkina TV, Sineokii SP, Kritskii MS. Characterization of lipoxygenase from fungi of the genus Mortierella. Appl Biochem Microbiol 2001;37: 473-9. https://doi.org/10.1023/A:1010289806262
  33. Nagao K, Yanagita T. Bioactive lipids in metabolic syndrome. Prog Lipid Res 2008;47:127-46. https://doi.org/10.1016/j.plipres.2007.12.002
  34. Botha A, Paul I, Roux C, Kock JL, Coetzee DJ, Strauss T, Maree C. An isolation procedure for arachidonic acid producing Mortierella species. Antonie Van Leeuwenhoek 1999;75;253-6. https://doi.org/10.1023/A:1001848709005
  35. Davies JL, Ngeleka M, Wobeser GA. Systemic infection with Mortierella wolfii following abortion in a cow. Can Vet J 2010;51:1391-3.
  36. Dokdo [Internet]. Daegu: Gyeongsangbuk-do Province; 2015 [cited 2015 Oct 1]. Available from: http://en.dokdo.go.kr/pages/s03/page.html?mc=7180.