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Matrix metalloproteinase-13 downregulation and potential cartilage protective action of the Korean Red Ginseng preparation

  • Lee, Je Hyeong (College of Pharmacy, Kangwon National University) ;
  • Shehzad, Omer (College of Pharmacy, Seoul National University) ;
  • Ko, Sung Kwon (Department of Oriental Medical Food and Nutrition, Semyung University) ;
  • Kim, Yeong Shik (College of Pharmacy, Seoul National University) ;
  • Kim, Hyun Pyo (College of Pharmacy, Kangwon National University)
  • Received : 2014.05.01
  • Accepted : 2014.06.25
  • Published : 2015.01.15

Abstract

Background: The present study was designed to prepare and find the optimum active preparation or fraction from Korea Red Ginseng inhibiting matrix metalloproteinase-13 (MMP-13) expression, because MMP-13 is a pivotal enzyme to degrade the collagen matrix of the joint cartilage. Methods: From total red ginseng ethanol extract, n-BuOH fraction (total ginsenoside-enriched fraction), ginsenoside diol-type-enriched fraction (GDF), and ginsenoside triol-type-enriched fraction (GTF) were prepared, and ginsenoside diol type-/F4-enriched fraction (GDF/F4) was obtained from Panax ginseng leaf extract. Results: The n-BuOH fraction, GDF, and GDF/F4 clearly inhibited MMP-13 expression compared to interleukin-$1{\beta}$-treated SW1353 cells (human chondrosarcoma), whereas the total extract and ginsenoside diol-type-enriched fraction did not. In particular, GDF/F4, the most effective inhibitor, blocked the activation of p38 mitogen-activated protein kinase (p38 MAPK), c-Jun-activated protein kinase (JNK), and signal transducer and activator of transcription-1/2 (STAT-1/2) among the signal transcription pathways involved. Further, GDF/F4 also inhibited the glycosaminoglycan release from interleukin-$1{\alpha}$-treated rabbit cartilage culture (30.6% inhibition at $30{\mu}g/mL$). Conclusion: Some preparations from Korean Red Ginseng and ginseng leaves, particularly GDF/F4, may possess the protective activity against cartilage degradation in joint disorders, and may have potential as new therapeutic agents.

Keywords

References

  1. Roughley PJ. The structure and function of cartilage proteoglycans. Eur Cell Mater 2006;12:92-101. https://doi.org/10.22203/eCM.v012a11
  2. Hadler-Olsen E, Fadnes B, Sylte I, Uhlin-Hansen L, Winberg JO. Regulation of matrix metalloproteinase activity in health and disease. FEBS J 2011;278: 28-45. https://doi.org/10.1111/j.1742-4658.2010.07920.x
  3. Mitchell PG, Magna HA, Reeves LM, Lopresti-Morrow LL, Yocum SA, Rosner PJ, Geoghegan KF, Hambor JE. Cloning, expression, and type II collagenolytic activity of matrix metalloproteinase-13 from human osteoarthritic cartilage. J Clin Invest 1996;97:761-8. https://doi.org/10.1172/JCI118475
  4. Takaishi H, Kimura T, Dalah S, Okada Y, D'Armiento J. Joint disease and matrix metalloproteinases: a role for MMP-13. Curr Pharmacol Biotechnol 2008;9: 47-54. https://doi.org/10.2174/138920108783497659
  5. Wu JY, Gardner BH, Murphy CI, Seals JR, Kensil CR, Recchia J, Beltz GA, Newman GW, Newman MJ. Saponin adjuvant enhancement of antigen-specific immune responses to an experimental HIV-1 vaccine. J Immunol 1992;148:1519-25.
  6. Mochizuki M, Yoo CY, Matsuzawa K, Sato K, Saiki I, Tono-oka S, Samukawa K, Azuma I. Inhibitory effect of tumor metastasis in mice by saponins, ginsenosides Rb2, 20(R)- and 20(S)-ginsenoside Rg3, of red ginseng. Biol Pharm Bull 1995;18:1197-202. https://doi.org/10.1248/bpb.18.1197
  7. Nag SA, Qin JJ, Wang W, Wang MH, Wang H, Zhang R. Ginsenosides as anticancer agents: in vitro and in vivo activities, structure-activity relationships, and molecular mechanisms of action. Front Pharmacol 2012;3:25.
  8. Shehzad O, Khan S, Ha IJ, Park Y, Kim YS. Rational development of selection model for solvent gradients in single step separation of ginsenosides from Panax ginseng using high-speed counter-current chromatography. J Sep Sci 2012;35:1462-9. https://doi.org/10.1002/jssc.201200135
  9. Shehzad O, Kim HP, Kim YS. State-of-the art separation of ginsenosides from Korean white and red ginseng by countercurrent chromatography. Anal Bioanal Chem 2013;405:4523-30. https://doi.org/10.1007/s00216-012-6609-z
  10. So MW, Lee EJ, Lee HS, Koo BS, Kim YG, Lee CK, Yoo B. Protective effects of ginsenoside Rg3 on human osteoarthritic chondrocytes. Mod Rheumatol 2013;23:104-11. https://doi.org/10.3109/s10165-012-0635-8
  11. Lee JH, Lim H, Shehzad O, Kim YS, Kim HP. Ginsenosides from Korean red ginseng inhibit matrix metalloproteinase-13 expression in articular chondrocytes and prevent cartilage degradation. Eur J Pharmacol 2014;724:145-51. https://doi.org/10.1016/j.ejphar.2013.12.035
  12. Lim H, Kim HP. Matrix metalloproteinase-13 expression in IL-1${\beta}$-treated chondrocytes by activation of the p38 MAPK/c-Fos/AP-1 and JAK/STAT pathways. Arch Pharm Res 2011;34:109-17. https://doi.org/10.1007/s12272-011-0113-4
  13. Mossman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays. J Immunol Methods 1983;65:55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  14. Mengshol JA, Vincenti MP, Coon CI, Barchowsky A, Brincherhoff CE. Interleukin- 1 induction of collagenase 3 (matrix metalloproteinase 13) gene expression in chondrocytes requires p38, c-Jun N-terminal kinase, and nuclear factor ${\kappa}B$: differential regulation of collagenase 1 and collagenase 3. Arthritis Rheum 2000;43:801-11. https://doi.org/10.1002/1529-0131(200004)43:4<801::AID-ANR10>3.0.CO;2-4
  15. Wang CZ, Aung HH, Ni M, Wu JA, Tong R, Wicks S, He TC, Yuan CS. Red American ginseng: ginsenosides constituents and antiproliferative activities of heat-processed Panax quinquefolius roots. Planta Med 2007;73:669-74. https://doi.org/10.1055/s-2007-981524

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