Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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Histopathological Effects of Tissue Adhesives on Experimental Peripheral Nerve Transection Model in Rats

  • Altun, Idiris (Department of Neurosurgery, Kahramanmaras Sutcu Imam University Medical Faculty) ;
  • Ciralik, Harun (Department of Pathology, Kahramanmaras Sutcu Imam University Medical Faculty)
  • Received : 2015.07.07
  • Accepted : 2015.09.14
  • Published : 2015.12.28
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Abstract

Objective : Our aim was to evaluate the histopathological effects of tissue adhesives on peripheral nerve regeneration after experimental sciatic nerve transection in rats and to search whether these tissue adhesives may possess a therapeutic potential in peripheral nerve injuries. Methods : This experimental study was performed using 42 female Wistar-Albino rats distributed in 6 groups subsequent to transection of right sciatic nerves. Group I underwent external circumferential neurolysis; Group II received suture repair; Group III had local polymeric hydrogel based tissue adhesive administration; Group IV received suture repair and polymeric hydrogel based tissue adhesive application together; Group V had gelatin based tissue adhesive application and Group VI had suture repair and gelatin based tissue adhesive together. After a 6-week follow-up period, biopsies were obtained from site of neural injury and groups were compared with respect to histopathological scoring based on inflammatory, degenerative, necrotic and fibrotic changes. Results : There were remarkable differences between control group and study groups with respect to inflammation (p=0.001), degeneration (p=0.002), necrosis (p=0.007), fibrosis (p<0.001) and vascularity (p=0.001). Histopathological scores were similar between study groups and the only noteworthy difference was that Group V displayed a lower score for necrosis and higher score in terms of vascularization. Conclusion : Our results imply that tissue adhesives can be useful in repair of peripheral nerve injuries by decreasing the surgical trauma and shortening the duration of intervention. Results with gelatin based tissue adhesive are especially promising since more intense vascularity was observed in tissue after application. However, trials on larger series with longer durations of follow-up are essential for reaching more reliable conclusions.

Keywords

References

  1. Barton MJ, Morley JW, Stoodley MA, Lauto A, Mahns DA : Nerve repair : toward a sutureless approach. Neurosurg Rev 37 : 585-595, 2014 https://doi.org/10.1007/s10143-014-0559-1
  2. Boogaarts JD, Grotenhuis JA, Bartels RH, Beems T : Use of a novel absorbable hydrogel for augmentation of dural repair : results of a preliminary clinical study. Neurosurgery 57 (1 Suppl) : 146-151; discussion 146-151, 2005
  3. Chao HH, Torchiana DF : BioGlue : albumin/glutaraldehyde sealant in cardiac surgery. J Card Surg 18 : 500-503, 2003 https://doi.org/10.1046/j.0886-0440.2003.00304.x
  4. Cruz NI, Debs N, Fiol RE : Evaluation of fibrin glue in rat sciatic nerve repairs. Plast Reconstr Surg 78 : 369-373, 1986 https://doi.org/10.1097/00006534-198609000-00015
  5. Felix SP, Pereira Lopes FR, Marques SA, Martinez AM : Comparison between suture and fibrin glue on repair by direct coaptation or tubulization of injured mouse sciatic nerve. Microsurgery 33 : 468-477, 2013 https://doi.org/10.1002/micr.22109
  6. Lee SH, Park CW, Lee SG, Kim WK : Postoperative cervical cord compression induced by hydrogel dural sealant (DuraSeal(R)). Korean J Spine 10 : 44-46, 2013 https://doi.org/10.14245/kjs.2013.10.1.44
  7. Lemaire SA, Ochoa LN, Conklin LD, Schmittling ZC, Undar A, Clubb FJ Jr, et al. : Nerve and conduction tissue injury caused by contact with Bio Glue. J Surg Res 143 : 286-293, 2007 https://doi.org/10.1016/j.jss.2006.10.014
  8. Maragh H, Meyer BS, Davenport D, Gould JD, Terzis JK : Morphofunctional evaluation of fibrin glue versus microsuture nerve repairs. J Reconstr Microsurg 6 : 331-337, 1990 https://doi.org/10.1055/s-2007-1006838
  9. Narakas A : The use of fibrin glue in repair of peripheral nerves. Orthop Clin North Am 19 : 187-199, 1988
  10. Orhan Z, Cam K, Alper M, Ozturan K : The effects of extracorporeal shock waves on the rat Achilles tendon : is there a critical dose for tissue injury? Arch Orthop Trauma Surg 124 : 631-635, 2004 https://doi.org/10.1007/s00402-003-0598-5
  11. P M, S H, M D, M S : Advances of peripheral nerve repair techniques to improve hand function : a systematic review of literature. Open Orthop J 6 : 60-68, 2012 https://doi.org/10.2174/1874325001206010060
  12. Renno WM, Al-Maghrebi M, Alshammari A, George P : (-)-Epigallocatechin-3-gallate (EGCG) attenuates peripheral nerve degeneration in rat sciatic nerve crush injury. Neurochem Int 62 : 221-231, 2013 https://doi.org/10.1016/j.neuint.2012.12.018
  13. Sener I, Bereket C, Arslan G, Ozkan N, Ozdemir M, Mercan U, et al. : The effect of hemostatic agents and tissue adhesive on injured peripheral nerve healing in rats - Part I. Electrophysiological study. Adv Clin Exp Med 24 : 23-29, 2015 https://doi.org/10.17219/acem/38157
  14. Smahel J, Meyer VE, Bachem U : Glueing of peripheral nerves with fibrin : experimental studies. J Reconstr Microsurg 3 : 211-220, 1987 https://doi.org/10.1055/s-2007-1006987
  15. Webber C, Zochodne D : The nerve regenerative microenvironment : early behavior and partnership of axons and Schwann cells. Exp Neurol 223 : 51-59, 2010 https://doi.org/10.1016/j.expneurol.2009.05.037
  16. Yeh DL, Bushley DM, Kim T : Treatment of traumatic LASIK flap dislocation and epithelial ingrowth with fibrin glue. Am J Ophthalmol 141 : 960-962, 2006 https://doi.org/10.1016/j.ajo.2005.12.001
  17. Yin ZS, Zhang H, Bo W, Gao W : Erythropoietin promotes functional recovery and enhances nerve regeneration after peripheral nerve injury in rats. AJNR Am J Neuroradiol 31 : 509-515, 2010 https://doi.org/10.3174/ajnr.A1820

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